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Na+/H+ Exchanger 2 (NHE-2) Antibody (ANX-002-200UL) in IHC (P)
Expression ofNa+/H+exchanger 2 in rat colon - Immunohistochemical staining of rat colon sections (paraffin-embedded) using Anti-Na+/H+Exchanger 2 (NHE-2) Antibody (#ANX-002), (1:50). A. NHE-2 staining is shown in red. B. Cell nuclei were labeled with DAPI (blue). C. Merge of the two images.
Product Details
ANX-002-200UL
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse,
Rat
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
(C)RASEPGNRKGRLGNEK, corresponding to amino acid residues 797-812 of rat NHE-2, Intracellular, C-terminus
Conjugate
Form
Lyophilized
Concentration
0.8 mg/mL
Purification
Antigen affinity chromatography
Storage buffer
PBS, pH 7.4, with 1% BSA
Contains
0.05% sodium azide
Storage conditions
-20°C, Avoid Freeze/Thaw Cycles
Shipping conditions
Ambient (domestic); Wet ice (international)
Product Specific Information
Reconstitution: 25 µL, 50 µL or 0.2 mL double distilled water (DDW), depending on the sample size. The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20C. The reconstituted solution can be stored at 4C, protected from the light, for up to 1 week. For longer periods, small aliquots should be stored at -20C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 X g 5 min).
Target Information
Involved in pH regulation to eliminate acids generated by active metabolism or to counter adverse environmental conditions. Major proton extruding system driven by the inward sodium ion chemical gradient. Seems to play an important role in colonic sodium absorption.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: H7; Na(+)/H(+) exchanger 2; Na/H ion exchanger; NHE-2; Sodium/hydrogen exchanger; Sodium/hydrogen exchanger 2; Solute carrier family 9 (sodium/hydrogen exchanger 2), antiporter 2, Na+/H+ (Na+/H+ exchanger 2); solute carrier family 9 (sodium/hydrogen exchanger), member 2; Solute carrier family 9 member 2; solute carrier family 9, member 2
Gene Aliases: 2210416H12Rik; 4932415O19Rik; AV333884; NHE2; SLC9A2
UniProt ID: (Human) Q9UBY0, (Mouse) Q3ZAS0, (Rat) P48763
Entrez Gene ID: (Human) 6549, (Mouse) 226999, (Rat) 24783
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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