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Invitrogen
Nebulin Monoclonal Antibody (NB2)
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Nebulin Antibody (MA5-49308) in WB
Western blot analysis of Nebulin in various lysates. Samples were incubated with Nebulin monoclonal antibody (Product # MA5-49308) at 0. 5 µg/mL. Lanes: Mouse Skeletal Muscle Tissue Lysate at 50 µg. Predicted bind size: 773KD; Observed bind size: 773KD.
Product Details
MA5-49308
Product Specifications
Species Reactivity
Chicken
Host/Isotype
Mouse
/ IgG1
Class
Monoclonal
Type
Antibody
Clone
NB2
Immunogen
Chicken breast nebulin.
Conjugate
Form
Lyophilized
Concentration
Conc. Not Determined
Storage buffer
ascites/1.2% sodium acetate with 2mg BSA
Contains
0.01mg sodium azide
Storage conditions
-20°C
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
AB_3074966
Product Specific Information
Adding 1 mL of PBS buffer will yield a concentration of 100 µg/mL.
Positive Control - WB: Mouse Skeletal Muscle Tissue.|Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.
Target Information
This gene encodes nebulin, a giant protein component of the cytoskeletal matrix that coexists with the thick and thin filaments within the sarcomeres of skeletal muscle. In most vertebrates, nebulin accounts for 3 to 4% of the total myofibrillar protein. The encoded protein contains approximately 30-amino acid long modules that can be classified into 7 types and other repeated modules. Protein isoform sizes vary from 600 to 800 kDa due to alternative splicing that is tissue-, species-, and developmental stage-specific. Of the 183 exons in the nebulin gene, at least 43 are alternatively spliced, although exons 143 and 144 are not found in the same transcript. Mutations in this gene are associated with recessive nemaline myopathy.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: DKFZp686C1456; FLJ11505; FLJ36536; FLJ39568; FLJ39584
Entrez Gene ID: (Chicken) 374027
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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