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Nesprin 1 Antibody (MA1-13082) in WB
Western blot analysis of CPG2 is shown using adult rat brain fractions and whole cell lysates from mock or CPG2 transfected HEK293T cells (kindly provided by Elly Nedivi's lab, MIT, Cambridge, MA) onto a 4-20% Tris-HCl polyacrylamide gel. Proteins were transferred to a PVDF membrane and blocked with 5% Milk/TBST for at least 1 hour. Membranes were probed with a mouse monoclonal antibody recognizing CPG2 (Product # MA1-13082) at a dilution of 1:1000 overnight at 4°C on a rocking platform. Membranes were washed in TBS-0.1%Tween 20 and probed... View More
Product Details
MA1-13082
Product Specifications
Species Reactivity
Mouse,
Rat
Host/Isotype
Mouse
/ IgG1
Class
Monoclonal
Type
Antibody
Clone
6A
Immunogen
Recombinant protein containing residues RKLESTLTGLEQSRERQERR
Conjugate
Form
Liquid
Concentration
1 mg/mL
Purification
Protein G
Storage buffer
PBS with 1mg/mL BSA
Contains
0.05% sodium azide
Storage conditions
-20°C
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
AB_2536838
Target Information
There are two genes encoding members of a new family of type II integral membrane proteins. Both are ubiquitously expressed, and tissue-specific alternative mRNA initiation and splicing generate at least two major isoforms of each protein, with the smaller isoforms being truncated at the N-terminus. These proteins are called nesprin-l and -2 for nuclear _envelope _spectrin repeat, as they are characterized by the presence of multiple, clustered spectrin repeats, bipartite nuclear localization sequences and a conserved C-terminal, single transmembrane domain. Transient transfection of EGFP-fusion expression constructs demonstrated their localization to the nuclear membrane with a novel C-terminal, TM-domain-containing sequence essential for perinuclear localization. Nesprin-l is developmentally regulated in both smooth and skeletal muscle and is relocalized from the nuclear envelope to the nucleus and cytoplasm during C2Cl2 myoblast differentiation. Nesprins may function as 'dystrophins of the nucleus' to maintain nuclear organization and structura integrity.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: DKFZp781J13156; Enaptin; enaptin165; FLJ30878; FLJ41140; KASH domain-containing protein 1; KASH1; Myne-1; Myocyte nuclear envelope protein 1; nesprin 1; Nesprin-1; Nuclear envelope spectrin repeat protein 1; RP1-130E4.2; synaptic nuclear envelope 1; Synaptic nuclear envelope protein 1; SYNE 1; Syne-1; syne-1 fragment
Gene Aliases: 8B; A330049M09Rik; BE692247; C130039F11Rik; CPG2; mKIAA1756; Myne-1; Myne1; Syne1
UniProt ID: (Mouse) Q6ZWR6
Entrez Gene ID: (Mouse) 64009, (Rat) 499010
Golgi organization
establishment of nucleus localization
muscle cell differentiation
positive regulation of receptor-mediated endocytosis
regulation of dendrite morphogenesis
cytoskeletal anchoring at nuclear membrane
nuclear matrix anchoring at nuclear membrane
brain development
response to light stimulus
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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