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Rockland
Nestin Polyclonal Antibody
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Nestin Antibody (600-401-417) in IHC (P)
Immunohistochemistry with anti-nestin antibody showing nestin staining in cytoplasm of ductal epithelium of human salivary gland (B) and in nucleus and cytoplasm of human breast tissue (C). Formalin fixed/paraffin embedded sections were subjected to heat induced epitope retrieval (HIER) at pH 6.2 and then incubated with rabbit anti-nestin antibody at 4.0 µg/ml for 60 minutes. The reaction was developed usi... View More
Product Details
600-401-417
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
Anti-Nestin affinity purified antibody was prepared from whole rabbit serum produced by repeated immunizations with a recombinant protein corresponding to amino acids 1484-1500 of human Nestin protein.
Conjugate
Form
Liquid
Concentration
1 mg/mL
Purification
Affinity chromatography
Storage buffer
0.02M potassium phosphate, pH 7.2, with 0.15M NaCl
Contains
0.01% sodium azide
Storage conditions
-20°C, Avoid Freeze/Thaw Cycles
Shipping conditions
Wet ice
Product Specific Information
Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
Nestin antibody is directed against human Nestin protein. A BLAST analysis was used to suggest cross reactivity with Nestin protein from human and macaque (100% homology). Based on protein sequence homology also expect partial reactivity against Nestin homologues from rabbit (94%), mouse (reactivity shown above), rat and hamster (88%), and bovine and dog (82%). Reactivity against homologues from other sources is not known.
Target Information
Nestin is a Class VI intermediate filament abundantly produced in the developing central nervous system (CNS) and in early embryonic neuroepithelial stem cells. Nestin has been widely used as a predominant marker for stem/progenitor cells, glioma cells, and tumor endothelial cells. Also, Nestin is a superior angiogenic marker to evaluate neovascularity of endothelial cells in tumors. Highly specific antibodies to human Nestin can aid in characterizing progenitor cells differentiating into distinct lineages, in enhancing the therapeutic potential of human neural stem/progenitor cells in the treatment of CNS diseases or injury, and in identifying neuroepithelial tumor cells. Nestin expression has been observed in pancreatic hematopoietic stem cell populations, and is considered a novel angiogenesis marker of proliferating endothelial cells in colorectal cancer tissue.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: FLJ21841; intermediate filament protein; Nestin
Gene Aliases: AA166324; C78523; ESTM46; Nbla00170; NES
UniProt ID: (Human) P48681, (Mouse) Q6P5H2
Entrez Gene ID: (Human) 10763, (Mouse) 18008
multicellular organism development
nervous system development
negative regulation of neuron apoptotic process
cell projection morphogenesis
G2/M transition of mitotic cell cycle
central nervous system development
brain development
positive regulation of intermediate filament depolymerization
embryonic camera-type eye development
negative regulation of protein binding
negative regulation of catalytic activity
stem cell proliferation
positive regulation of neural precursor cell proliferation
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