Invitrogen

Nestin Monoclonal Antibody (10C2), eBioscience™

Nestin Antibody in Immunocytochemistry (ICC/IF)

FIGURE: 1 / 16

Nestin Antibody (14-9843-82) in ICC/IF

Immunofluorescence analysis of Nestin was performed using 70% confluent log phase NSC cells. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 15 minutes, and blocked with 2% BSA for 45 minutes at room temperature. The cells were labeled with Nestin Monoclonal Antibody (10C2), eBioscience™ (Product # 14-9843-82) at 5 µg/mL in 0.1% BSA, incubated at 4 degree celsius overnight and then labeled with Donkey anti-Mouse IgG (H+L) Highly Cross-Adsorbed Secondary Antibody, Alexa Fluor Plus 488 ... View More

Nestin Antibody in Western Blot, Immunocytochemistry (WB, ICC/IF)

Nestin Antibody in Immunocytochemistry, Immunohistochemistry (Frozen) (ICC/IF, IHC (F))

Nestin Antibody in Immunohistochemistry (IHC)

Nestin Antibody in Flow Cytometry (Flow)

Product Image Gallery

Product Details

14-9843-82

Applications

Tested Dilution

Publications

Western Blot (WB)

1-5 µg/mL

View 2 publications 2 publications

Immunohistochemistry (IHC)

View 3 publications 3 publications

Immunohistochemistry (Paraffin) (IHC (P))

Assay-Dependent

Immunohistochemistry (Frozen) (IHC (F))

Assay-Dependent

View 1 publication 1 publication

Immunocytochemistry (ICC/IF)

5 µg/mL

View 6 publications 6 publications

Flow Cytometry (Flow)

Assay-Dependent

View 1 publication 1 publication

Product Specifications

Species Reactivity

Human

Published species

Human, Rat

Host/Isotype

Mouse / IgG1

Class

Monoclonal

Type

Antibody

Clone

10C2

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

0.5 mg/mL

Purification

Affinity chromatography

Storage buffer

PBS, pH 7.2

Contains

0.09% sodium azide

Storage conditions

4°C

Shipping conditions

Ambient (domestic); Wet ice (international)

AB_1548837

Product Specific Information

Description: The monoclonal antibody 10C2 recognizes human nestin (residues 1464-1614). Nestin is a 220-240 kDa Class VI intermediate filament protein that is expressed in stem cells of the developing nervous system. Expression has also been found in pancreatic islet and mesenchymal precursors in addition to cells with angiogenic potential. Upon differentiation, nestin-containing filaments are replaced by cell type-specific intermediate filament proteins, such as GFAP. Expression has been identified in a variety of cancerous cells including glioblastomas and pancreatic tumors. No cross-reactivity to rodent nestins has been observed using the 10C2 antibody.

Applications Reported: This 10C2 antibody has been reported for use in immunocytochemistry, microscopy, western blotting, immunohistochemical staining of frozen tissue sections, immunohistochemical staining of formalin-fixed paraffin embedded tissue sections, and flow cytometric analysis.

Applications Tested: This 10C2 antibody has been tested by western blot on SDS-reduced and non-reduced lysates from U251 cells and can be used at less than or equal to 5 µg/mL. The 10C2 antibody has also been tested by immunocytochemistry on formaldehyde-fixed and permeabilized SKNSH cells and can be used at less than or equal to 5 µg/mL. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.

Purity: Greater than 90%, as determined by SDS-PAGE.

Aggregation: Less than 10%, as determined by HPLC.

Filtration: 0.2 µm post-manufacturing filtered.

Target Information

Nestin is a Class VI intermediate filament abundantly produced in the developing central nervous system (CNS) and in early embryonic neuroepithelial stem cells. Nestin has been widely used as a predominant marker for stem/progenitor cells, glioma cells, and tumor endothelial cells. Also, Nestin is a superior angiogenic marker to evaluate neovascularity of endothelial cells in tumors. Highly specific antibodies to human Nestin can aid in characterizing progenitor cells differentiating into distinct lineages, in enhancing the therapeutic potential of human neural stem/progenitor cells in the treatment of CNS diseases or injury, and in identifying neuroepithelial tumor cells. Nestin expression has been observed in pancreatic hematopoietic stem cell populations, and is considered a novel angiogenesis marker of proliferating endothelial cells in colorectal cancer tissue.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

Pannexin 1 Influences Lineage Specification of Human iPSCs.

Frontiers in cell and developmental biology

Noort RJ,Christopher GA,Esseltine JL

14-9843 was used in Immunocytochemistry-immunoflourescence to show that PANX1 knockout iPSCs are fully capable of differentiating toward each specific lineage when exposed to the appropriate external signaling pressures, suggesting that although PANX1 influences germ lineage specification, it is not essential to this process.

Tue May 04 00:00:00 UTC 2021

Pannexin 1 Influences Lineage Specification of Human iPSCs.

Frontiers in cell and developmental biology

Noort RJ,Christopher GA,Esseltine JL

Tue May 04 00:00:00 UTC 2021

Pannexin 1 Influences Lineage Specification of Human iPSCs.

Frontiers in cell and developmental biology

Noort RJ,Christopher GA,Esseltine JL

Tue May 04 00:00:00 UTC 2021

Initiation of Pancreatic Cancer: The Interplay of Hyperglycemia and Macrophages Promotes the Acquisition of Malignancy-Associated Properties in Pancreatic Ductal Epithelial Cells.

International journal of molecular sciences

Otto L,Rahn S,Daunke T,Walter F,Winter E,Möller JL,Rose-John S,Wesch D,Schäfer H,Sebens S

14-9843-82 was used in Western Blot to investigate whether concomitant exposure to hyperglycemia and macrophages aggravates epithelial-mesenchymal transition-associated alterations in pancreatic ductal adenocarcinoma.

Tue May 11 00:00:00 UTC 2021

Initiation of Pancreatic Cancer: The Interplay of Hyperglycemia and Macrophages Promotes the Acquisition of Malignancy-Associated Properties in Pancreatic Ductal Epithelial Cells.

International journal of molecular sciences

Otto L,Rahn S,Daunke T,Walter F,Winter E,Möller JL,Rose-John S,Wesch D,Schäfer H,Sebens S

Tue May 11 00:00:00 UTC 2021

Connexin 43 Gene Ablation Does Not Alter Human Pluripotent Stem Cell Germ Lineage Specification.

Biomolecules

Christopher GA,Noort RJ,Esseltine JL

14-9843-82 was used in Immunocytochemistry to present the differential transcript expression for 11 of the 21 Cx isoforms in iPSCs and the three germ layers.

Wed Dec 22 00:00:00 UTC 2021

Connexin 43 Gene Ablation Does Not Alter Human Pluripotent Stem Cell Germ Lineage Specification.

Biomolecules

Christopher GA,Noort RJ,Esseltine JL

14-9843-82 was used in Immunocytochemistry to present the differential transcript expression for 11 of the 21 Cx isoforms in iPSCs and the three germ layers.

Wed Dec 22 00:00:00 UTC 2021

Connexin 43 Gene Ablation Does Not Alter Human Pluripotent Stem Cell Germ Lineage Specification.

Biomolecules

Christopher GA,Noort RJ,Esseltine JL

14-9843-82 was used in Immunocytochemistry to present the differential transcript expression for 11 of the 21 Cx isoforms in iPSCs and the three germ layers.

Wed Dec 22 00:00:00 UTC 2021

Anti-oncogenic activities exhibited by paracrine factors of MSCs can be mediated by modulation of KITLG and DKK1 genes in glioma SCs in vitro.

Molecular therapy oncolytics

Aslam N,Abusharieh E,Abuarqoub D,Ali D,Al-Hattab D,Wehaibi S,Al-Kurdi B,Jamali F,Alshaer W,Jafar H,Awidi AS

14-9843-82 was used in Flow Cytometry, Immunocytochemistry, Immunohistochemistry (Frozen), Immunohistochemistry (Paraffin), Western Blot to conclude that different types of mesenchymal stem cells possess antitumor properties and their paracrine factors, in combination with anti-immune modalities, can provide practical therapeutic targets for glioblastoma treatment.

Fri Mar 26 00:00:00 UTC 2021

Herpes simplex virus type 1 infection leads to neurodevelopmental disorder-associated neuropathological changes.

PLoS pathogens

Qiao H,Guo M,Shang J,Zhao W,Wang Z,Liu N,Li B,Zhou Y,Wu Y,Chen P

Published figure using Nestin monoclonal antibody (Product # 14-9843-82) in Immunohistochemistry

Thu Oct 01 00:00:00 UTC 2020

Herpes simplex virus type 1 infection leads to neurodevelopmental disorder-associated neuropathological changes.

PLoS pathogens

Qiao H,Guo M,Shang J,Zhao W,Wang Z,Liu N,Li B,Zhou Y,Wu Y,Chen P

Published figure using Nestin monoclonal antibody (Product # 14-9843-82) in Immunohistochemistry

Thu Oct 01 00:00:00 UTC 2020

Molecular analyses of glioblastoma stem-like cells and glioblastoma tissue.

PloS one

Wallenborn M,Xu LX,Kirsten H,Rohani L,Rudolf D,Ahnert P,Schmidt C,Schulz RM,Richter M,Krupp W,Mueller W,Johnson AA,Meixensberger J,Holland H

14-9843-82 was used in Immunocytochemistry to understand glioblastoma stem-like cells and their relation to malignant disease.

Tue Sep 15 00:00:00 UTC 2020

Emulating the early phases of human tooth development in vitro.

Scientific reports

Rosowski J,Bräunig J,Amler AK,Strietzel FP,Lauster R,Rosowski M

14-9843-82 was used in Immunohistochemistry to reproduce fundamental steps of human tooth organogenesis in vitro using human dental pulp cells.

Tue May 07 00:00:00 UTC 2019

Protective effects of primary neural stem cell treatment in ischemic stroke models.

Experimental and therapeutic medicine

Yu X,Wang X,Zeng S,Tuo X

14-9843-82 was used in Immunocytochemistry to examine the protective role of neural stem cell (NSC) administration in oxygen-glucose deprivation (OGD) injury and ischemic stroke animal models.

Sat Sep 01 00:00:00 UTC 2018

Sphere-Derived Multipotent Progenitor Cells Obtained From Human Oral Mucosa Are Enriched in Neural Crest Cells.

Stem cells translational medicine

Abe S,Yamaguchi S,Sato Y,Harada K

14-9843 was used in Immunohistochemistry-immunofluorescence to isolate neural crest stem cells from oral mucosa using the neurosphere technique and establish in vivo bone tissue regeneration methods.

Fri Jan 01 00:00:00 UTC 2016

Histone modification profiling reveals differential signatures associated with human embryonic stem cell self-renewal and differentiation.

Proteomics

Bhanu NV,Sidoli S,Garcia BA

Published figure using Nestin monoclonal antibody (Product # 14-9843-82) in Flow Cytometry

Mon Feb 01 00:00:00 UTC 2016

Bioinformatics

Protein Aliases: FLJ21841; Nestin

View more View less

Gene Aliases: Nbla00170; NES

View more View less

UniProt ID: (Human) P48681

View more View less

Entrez Gene ID: (Human) 10763

View more View less

Function(s)

molecular_function structural molecule activity intermediate filament binding

Process(es)

G2/M transition of mitotic cell cycle central nervous system development brain development positive regulation of intermediate filament depolymerization embryonic camera-type eye development negative regulation of protein binding negative regulation of catalytic activity negative regulation of neuron apoptotic process cell projection morphogenesis stem cell proliferation positive regulation of neural precursor cell proliferation

Performance Guarantee

If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

Learn more

Nestin Monoclonal Antibody (10C2), eBioscience™

Nestin Antibody (14-9843-82) in ICC/IF

Product Details

14-9843-82

Western Blot (WB)

Immunohistochemistry (IHC)

Immunohistochemistry (Paraffin) (IHC (P))

Immunohistochemistry (Frozen) (IHC (F))

Immunocytochemistry (ICC/IF)

Flow Cytometry (Flow)

Species Reactivity

Published species

Host/Isotype

Class

Type

Clone

Conjugate

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

Shipping conditions

RRID

Product Specific Information

Target Information

Bioinformatics

Performance Guarantee

We're here to help

Your items have has been added!