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          Clicking the images or links will redirect you to a website hosted by BenchSci that provides third-party scientific content. Neither the content nor the BenchSci technology and processes for selection have been evaluated by us; we are providing them as-is and without warranty of any kind, including for use or application of the Thermo Fisher Scientific products presented.

          • Primary Antibodies ›
          • Nestin Antibodies

          Invitrogen

          Nestin Monoclonal Antibody (10C2), Alexa Fluor™ 488, eBioscience™

          10 Published Figures
          11 References
          View all (58) Nestin antibodies

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          Datasheet
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          Cite Nestin Monoclonal Antibody (10C2), Alexa Fluor™ 488, eBioscience™

          • Antibody Testing Data (1)
          • Published Figures (10)
          Nestin Antibody in Immunocytochemistry (ICC/IF)
          Group 53 Created with Sketch.
          Nestin Antibody in Immunocytochemistry (ICC/IF)
          Group 53 Created with Sketch.

          FIGURE: 1 / 11

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          Nestin Antibody (53-9843-82) in ICC/IF

          Immunocytochemistry of fixed and permeabilized SKNSH cells using 10 µg/mL of Mouse IgG1 kappa Isotype Control Alexa Fluor® 488 (Product # 53-4714-42) (left) or 10 µg/mL Anti-Human Nestin Alexa Fluor® 488 (right). Nuclei are stained with DAPI. {{ $ctrl.currentElement.advancedVerification.fullName }} validation info. View more
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          View Product

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          Nestin Antibody in Immunocytochemistry (ICC/IF)
          Nestin Antibody in Western Blot, Immunocytochemistry (WB, ICC/IF)
          Nestin Antibody in Immunocytochemistry (ICC/IF)
          Nestin Antibody in Immunocytochemistry, Immunohistochemistry (Frozen) (ICC/IF, IHC (F))
          Nestin Antibody in Immunocytochemistry (ICC/IF)
          Nestin Antibody in Immunocytochemistry (ICC/IF)
          Nestin Antibody in Immunohistochemistry (IHC)
          Nestin Antibody in Immunohistochemistry (IHC)
          Nestin Antibody in Western Blot (WB)
          Nestin Antibody in Flow Cytometry (Flow)
          Nestin Antibody in Flow Cytometry (Flow)

          Product Details

          53-9843-82

          Applications
          Tested Dilution
          Publications

          Western Blot (WB)

          -
          View 2 publications 2 publications

          Immunohistochemistry (IHC)

          Assay-Dependent
          View 2 publications 2 publications

          Immunohistochemistry (Frozen) (IHC (F))

          -
          View 1 publication 1 publication

          Immunocytochemistry (ICC/IF)

          10 µg/mL
          View 3 publications 3 publications

          Flow Cytometry (Flow)

          Assay-Dependent
          View 3 publications 3 publications
          Product Specifications

          Species Reactivity

          Human

          Published species

          Human

          Host/Isotype

          Mouse / IgG1

          Recommended Isotype Control

          Mouse IgG1 kappa Isotype Control (P3.6.2.8.1), Alexa Fluor™ 488, eBioscience™

          Class

          Monoclonal

          Type

          Antibody

          Clone

          10C2

          Conjugate

          Alexa Fluor™ 488 Alexa Fluor™ 488 Alexa Fluor™ 488
          • Unconjugated
          • eFluor 570
          • Request custom conjugation

          Excitation/Emission Max

          499/520 nm View spectra spectra

          Form

          Liquid

          Concentration

          0.5 mg/mL

          Purification

          Affinity chromatography

          Storage buffer

          PBS, pH 7.2

          Contains

          0.09% sodium azide

          Storage conditions

          4°C, store in dark, DO NOT FREEZE!

          Shipping conditions

          Ambient (domestic); Wet ice (international)

          RRID

          AB_1834346

          Product Specific Information

          Description: The monoclonal antibody 10C2 recognizes human nestin (residues 1464-1614). Nestin is a 220-240 kDa Class VI intermediate filament protein that is expressed in stem cells of the developing nervous system. Expression has also been found in pancreatic islet and mesenchymal precursors in addition to cells with angiogenic potential. Upon differentiation, nestin-containing filaments are replaced by cell type-specific intermediate filament proteins, such as GFAP. Expression has been identified in a variety of cancerous cells including glioblastomas and pancreatic tumors. No cross-reactivity to rodent nestins has been observed using the 10C2 antibody.

          Applications Reported: This 10C2 antibody has been reported for use in microscopy, immunocytochemistry, immunohistochemical staining, and flow cytometric analysis.

          Applications Tested: This 10C2 antibody has been tested by immunocytochemistry of formaldehyde-fixed and permeabilized cells. This can be used at less than or equal to 10 µg/mL. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.

          Excitation: 488 nm; Emission: 519 nm; Laser: Blue Laser.

          Filtration: 0.2 µm post-manufacturing filtered.

          Target Information

          Nestin is a Class VI intermediate filament abundantly produced in the developing central nervous system (CNS) and in early embryonic neuroepithelial stem cells. Nestin has been widely used as a predominant marker for stem/progenitor cells, glioma cells, and tumor endothelial cells. Also, Nestin is a superior angiogenic marker to evaluate neovascularity of endothelial cells in tumors. Highly specific antibodies to human Nestin can aid in characterizing progenitor cells differentiating into distinct lineages, in enhancing the therapeutic potential of human neural stem/progenitor cells in the treatment of CNS diseases or injury, and in identifying neuroepithelial tumor cells. Nestin expression has been observed in pancreatic hematopoietic stem cell populations, and is considered a novel angiogenesis marker of proliferating endothelial cells in colorectal cancer tissue.

          For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

          How to use the Panel Builder

          Watch the video to learn how to use the Invitrogen Flow Cytometry Panel Builder to build your next flow cytometry panel in 5 easy steps.

          Bioinformatics

          Protein Aliases: FLJ21841; Nestin

          View more View less

          Gene Aliases: Nbla00170; NES

          View more View less

          UniProt ID: (Human) P48681

          View more View less

          Entrez Gene ID: (Human) 10763

          View more View less

          Function(s)
          molecular_function structural molecule activity intermediate filament binding
          Process(es)
          G2/M transition of mitotic cell cycle central nervous system development brain development positive regulation of intermediate filament depolymerization embryonic camera-type eye development negative regulation of protein binding negative regulation of catalytic activity negative regulation of neuron apoptotic process cell projection morphogenesis stem cell proliferation positive regulation of neural precursor cell proliferation
          It has to be done as per old AB suggested Products section.
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          Performance Guarantee

          If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

          Learn more
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          We're here to help

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