Invitrogen

Nestin Monoclonal Antibody (10C2), Alexa Fluor™ 488, eBioscience™

10 Published Figures

11 References

View all (58) Nestin antibodies

Datasheet

Protocols

Questions & Answers

Datasheet

Protocols

Questions & Answers

Antibody Testing Data (1)
Published Figures (10)

Nestin Antibody in Immunocytochemistry (ICC/IF)

FIGURE: 1 / 11

Nestin Antibody (53-9843-82) in ICC/IF

Immunocytochemistry of fixed and permeabilized SKNSH cells using 10 µg/mL of Mouse IgG1 kappa Isotype Control Alexa Fluor® 488 (Product # 53-4714-42) (left) or 10 µg/mL Anti-Human Nestin Alexa Fluor® 488 (right). Nuclei are stained with DAPI.

Nestin Antibody in Western Blot, Immunocytochemistry (WB, ICC/IF)

Nestin Antibody in Immunocytochemistry, Immunohistochemistry (Frozen) (ICC/IF, IHC (F))

Nestin Antibody in Immunohistochemistry (IHC)

Nestin Antibody in Flow Cytometry (Flow)

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Product Details

53-9843-82

Applications

Tested Dilution

Publications

Western Blot (WB)

View 2 publications 2 publications

Immunohistochemistry (IHC)

Assay-Dependent

View 2 publications 2 publications

Immunohistochemistry (Frozen) (IHC (F))

View 1 publication 1 publication

Immunocytochemistry (ICC/IF)

10 µg/mL

View 3 publications 3 publications

Flow Cytometry (Flow)

Assay-Dependent

View 3 publications 3 publications

Product Specifications

Species Reactivity

Human

Published species

Human

Host/Isotype

Mouse / IgG1

Recommended Isotype Control

Mouse IgG1 kappa Isotype Control (P3.6.2.8.1), Alexa Fluor™ 488, eBioscience™

Class

Monoclonal

Type

Antibody

Clone

10C2

Conjugate

Alexa Fluor™ 488 Alexa Fluor™ 488 Alexa Fluor™ 488

Excitation/Emission Max

499/520 nm View spectra

Form

Liquid

Concentration

0.5 mg/mL

Purification

Affinity chromatography

Storage buffer

PBS, pH 7.2

Contains

0.09% sodium azide

Storage conditions

4°C, store in dark, DO NOT FREEZE!

Shipping conditions

Ambient (domestic); Wet ice (international)

AB_1834346

Product Specific Information

Description: The monoclonal antibody 10C2 recognizes human nestin (residues 1464-1614). Nestin is a 220-240 kDa Class VI intermediate filament protein that is expressed in stem cells of the developing nervous system. Expression has also been found in pancreatic islet and mesenchymal precursors in addition to cells with angiogenic potential. Upon differentiation, nestin-containing filaments are replaced by cell type-specific intermediate filament proteins, such as GFAP. Expression has been identified in a variety of cancerous cells including glioblastomas and pancreatic tumors. No cross-reactivity to rodent nestins has been observed using the 10C2 antibody.

Applications Reported: This 10C2 antibody has been reported for use in microscopy, immunocytochemistry, immunohistochemical staining, and flow cytometric analysis.

Applications Tested: This 10C2 antibody has been tested by immunocytochemistry of formaldehyde-fixed and permeabilized cells. This can be used at less than or equal to 10 µg/mL. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.

Excitation: 488 nm; Emission: 519 nm; Laser: Blue Laser.

Filtration: 0.2 µm post-manufacturing filtered.

Target Information

Nestin is a Class VI intermediate filament abundantly produced in the developing central nervous system (CNS) and in early embryonic neuroepithelial stem cells. Nestin has been widely used as a predominant marker for stem/progenitor cells, glioma cells, and tumor endothelial cells. Also, Nestin is a superior angiogenic marker to evaluate neovascularity of endothelial cells in tumors. Highly specific antibodies to human Nestin can aid in characterizing progenitor cells differentiating into distinct lineages, in enhancing the therapeutic potential of human neural stem/progenitor cells in the treatment of CNS diseases or injury, and in identifying neuroepithelial tumor cells. Nestin expression has been observed in pancreatic hematopoietic stem cell populations, and is considered a novel angiogenesis marker of proliferating endothelial cells in colorectal cancer tissue.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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Product References

Pannexin 1 Influences Lineage Specification of Human iPSCs.

Frontiers in cell and developmental biology

Noort RJ,Christopher GA,Esseltine JL

Published figure using Nestin monoclonal antibody (Product # 53-9843-82) in Immunocytochemistry

Tue May 04 00:00:00 UTC 2021

Pannexin 1 Influences Lineage Specification of Human iPSCs.

Frontiers in cell and developmental biology

Noort RJ,Christopher GA,Esseltine JL

Published figure using Nestin monoclonal antibody (Product # 53-9843-82) in Immunocytochemistry

Tue May 04 00:00:00 UTC 2021

Initiation of Pancreatic Cancer: The Interplay of Hyperglycemia and Macrophages Promotes the Acquisition of Malignancy-Associated Properties in Pancreatic Ductal Epithelial Cells.

International journal of molecular sciences

Otto L,Rahn S,Daunke T,Walter F,Winter E,Möller JL,Rose-John S,Wesch D,Schäfer H,Sebens S

Published figure using Nestin monoclonal antibody (Product # 53-9843-82) in Western Blot

Tue May 11 00:00:00 UTC 2021

Connexin 43 Gene Ablation Does Not Alter Human Pluripotent Stem Cell Germ Lineage Specification.

Biomolecules

Christopher GA,Noort RJ,Esseltine JL

Published figure using Nestin monoclonal antibody (Product # 53-9843-82) in Immunocytochemistry

Wed Dec 22 00:00:00 UTC 2021

Connexin 43 Gene Ablation Does Not Alter Human Pluripotent Stem Cell Germ Lineage Specification.

Biomolecules

Christopher GA,Noort RJ,Esseltine JL

Published figure using Nestin monoclonal antibody (Product # 53-9843-82) in Immunocytochemistry

Wed Dec 22 00:00:00 UTC 2021

Herpes simplex virus type 1 infection leads to neurodevelopmental disorder-associated neuropathological changes.

PLoS pathogens

Qiao H,Guo M,Shang J,Zhao W,Wang Z,Liu N,Li B,Zhou Y,Wu Y,Chen P

Published figure using Nestin monoclonal antibody (Product # 53-9843-82) in Immunocytochemistry

Thu Oct 01 00:00:00 UTC 2020

Herpes simplex virus type 1 infection leads to neurodevelopmental disorder-associated neuropathological changes.

PLoS pathogens

Qiao H,Guo M,Shang J,Zhao W,Wang Z,Liu N,Li B,Zhou Y,Wu Y,Chen P

Published figure using Nestin monoclonal antibody (Product # 53-9843-82) in Immunocytochemistry

Thu Oct 01 00:00:00 UTC 2020

Metalloproteinases ADAM10 and ADAM17 Mediate Migration and Differentiation in Glioblastoma Sphere-Forming Cells.

Molecular neurobiology

Siney EJ,Holden A,Casselden E,Bulstrode H,Thomas GJ,Willaime-Morawek S

53-9843 was used in Flow cytometry/Cell sorting to explore the role of the metalloproteinases, ADAM10 and ADAM17, on the adhesive and migratory properties of human glioma sphere-forming cells.

Sat Jul 01 00:00:00 UTC 2017

Sphere-Derived Multipotent Progenitor Cells Obtained From Human Oral Mucosa Are Enriched in Neural Crest Cells.

Stem cells translational medicine

Abe S,Yamaguchi S,Sato Y,Harada K

53-9843 was used in Immunohistochemistry-immunofluorescence to isolate neural crest stem cells from oral mucosa using the neurosphere technique and establish in vivo bone tissue regeneration methods.

Fri Jan 01 00:00:00 UTC 2016

Histone modification profiling reveals differential signatures associated with human embryonic stem cell self-renewal and differentiation.

Proteomics

Bhanu NV,Sidoli S,Garcia BA

53-9843 was used in Flow cytometry/Cell sorting to highlight subtle epigenetic signatures that allow the prediction of divergent lineage progression even in differentiating cells with similar phenotype and gene expression.

Mon Feb 01 00:00:00 UTC 2016

Histone modification profiling reveals differential signatures associated with human embryonic stem cell self-renewal and differentiation.

Proteomics

Bhanu NV,Sidoli S,Garcia BA

Mon Feb 01 00:00:00 UTC 2016

Establishment and characterization of primary glioblastoma cell lines from fresh and frozen material: a detailed comparison.

PloS one

Mullins CS,Schneider B,Stockhammer F,Krohn M,Classen CF,Linnebacher M

53-9843 was used in Flow cytometry/Cell sorting to evaluate a very simple cryopreservation procedure for GBM tissue prior to model establishment that will considerably reduce the logistic complexity.

Thu Aug 21 00:00:00 UTC 2014

Bioinformatics

Protein Aliases: FLJ21841; Nestin

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Gene Aliases: Nbla00170; NES

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UniProt ID: (Human) P48681

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Entrez Gene ID: (Human) 10763

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Function(s)

molecular_function structural molecule activity intermediate filament binding

Process(es)

G2/M transition of mitotic cell cycle central nervous system development brain development positive regulation of intermediate filament depolymerization embryonic camera-type eye development negative regulation of protein binding negative regulation of catalytic activity negative regulation of neuron apoptotic process cell projection morphogenesis stem cell proliferation positive regulation of neural precursor cell proliferation

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If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

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Nestin Monoclonal Antibody (10C2), Alexa Fluor™ 488, eBioscience™

Nestin Antibody (53-9843-82) in ICC/IF

Product Details

53-9843-82

Western Blot (WB)

Immunohistochemistry (IHC)

Immunohistochemistry (Frozen) (IHC (F))

Immunocytochemistry (ICC/IF)

Flow Cytometry (Flow)

Species Reactivity

Published species

Host/Isotype

Recommended Isotype Control

Class

Type

Clone

Conjugate

Excitation/Emission Max

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

Shipping conditions

RRID

Product Specific Information

Target Information

How to use the Panel Builder

Bioinformatics

Performance Guarantee

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