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          Clicking the images or links will redirect you to a website hosted by BenchSci that provides third-party scientific content. Neither the content nor the BenchSci technology and processes for selection have been evaluated by us; we are providing them as-is and without warranty of any kind, including for use or application of the Thermo Fisher Scientific products presented.

          • Primary Antibodies ›
          • Nestin Antibodies

          Invitrogen

          Nestin Monoclonal Antibody (10C2)

          Advanced Verification
          14 Published Figures
          41 References
          View all (55) Nestin antibodies

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          Datasheet
          Protocols
          Questions & Answers
          Datasheet
          Protocols
          Questions & Answers

          Cite Nestin Monoclonal Antibody (10C2)

          • Antibody Testing Data (19)
          • Published Figures (14)
          • Advanced Verification (3)
          Nestin Antibody in Immunocytochemistry (ICC/IF)
          Group 53 Created with Sketch.
          Nestin Antibody in Immunocytochemistry (ICC/IF)
          Group 53 Created with Sketch.

          FIGURE: 1 / 36

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          Nestin Antibody (MA1-110) in ICC/IF

          Immunofluorescent analysis of Nestin (green) in human neural stem cells derived from PD-3 iPSCs using Gibco® PSC Neural Induction Medium (Product # A1647801). The cells were fixed and permeabilized using Image-IT® Fixation/Permeabilization kit (Product # R37602), and blocked with the including blocking buffer for one hour at room temperature. Cells were stained with a Nestin monoclonal antibody (Product # MA1-110) at a dilution of 1:100 in blocking buffer for 3 hours at room temperature, and then incubated with a DyLight488-conjugated goat... View More {{ $ctrl.currentElement.advancedVerification.fullName }} validation info. View more
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          View Product

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          Nestin Antibody in Immunocytochemistry (ICC/IF)
          Nestin Antibody in Immunohistochemistry (IHC)
          Nestin Antibody in Western Blot (WB)
          Nestin Antibody in Flow Cytometry (Flow)
          Nestin Antibody in Immunocytochemistry (ICC/IF)
          Nestin Antibody in Flow Cytometry (Flow)
          Nestin Antibody in Immunocytochemistry (ICC/IF)
          Nestin Antibody in Immunocytochemistry (ICC/IF)
          Nestin Antibody in Immunocytochemistry (ICC/IF)
          Nestin Antibody in Immunocytochemistry (ICC/IF)
          Nestin Antibody in Immunocytochemistry (ICC/IF)
          Nestin Antibody in Immunohistochemistry (Frozen) (IHC (F))
          Nestin Antibody in Immunohistochemistry (IHC)
          Nestin Antibody in Immunohistochemistry (IHC)
          Nestin Antibody in Western Blot (WB)
          Nestin Antibody in Western Blot (WB)
          Nestin Antibody in Western Blot (WB)
          Nestin Antibody in Flow Cytometry (Flow)
          Nestin Antibody in Flow Cytometry (Flow)
          Nestin Antibody in Immunocytochemistry (ICC/IF)
          Nestin Antibody in Immunocytochemistry (ICC/IF)
          Nestin Antibody in Western Blot, Immunocytochemistry (WB, ICC/IF)
          Nestin Antibody in Immunocytochemistry (ICC/IF)
          Nestin Antibody in Immunocytochemistry (ICC/IF)
          Nestin Antibody in Immunocytochemistry (ICC/IF)
          Nestin Antibody in Immunocytochemistry (ICC/IF)
          Nestin Antibody in Immunocytochemistry (ICC/IF)
          Nestin Antibody in Immunocytochemistry (ICC/IF)
          Nestin Antibody in Immunocytochemistry (ICC/IF)
          Nestin Antibody in Immunocytochemistry (ICC/IF)
          Nestin Antibody in Immunohistochemistry (IHC)
          Nestin Antibody in Western Blot (WB)
          Nestin Antibody in Western Blot (WB)
          Nestin Antibody
          Nestin Antibody
          Nestin Antibody

          Product Details

          MA1-110

          Applications
          Tested Dilution
          Publications

          Western Blot (WB)

          1:500-1:1,500
          View 6 publications 6 publications

          Immunohistochemistry (IHC)

          -
          View 11 publications 11 publications

          Immunohistochemistry (Paraffin) (IHC (P))

          1:20-1:100
          View 2 publications 2 publications

          Immunohistochemistry (Frozen) (IHC (F))

          Assay-dependent
          -

          Immunocytochemistry (ICC/IF)

          1:100
          View 21 publications 21 publications

          Flow Cytometry (Flow)

          1 µg/test
          View 1 publication 1 publication
          Product Specifications

          Species Reactivity

          Human, Mouse

          Published species

          Human, Mouse

          Host/Isotype

          Mouse / IgG1, kappa

          Class

          Monoclonal

          Type

          Antibody

          Clone

          10C2

          Immunogen

          Human nestin protein corresponding to residues 1464-1614
          View immunogen

          Conjugate

          Unconjugated Unconjugated Unconjugated
          • Alexa Fluor 488 
          • Alexa Fluor 555 
          • Alexa Fluor 647 
          • Request custom conjugation

          Form

          Liquid

          Concentration

          1 mg/mL

          Purification

          Protein A

          Storage buffer

          PBS with 1mg/mL BSA, 30% glycerol

          Contains

          0.05% sodium azide

          Storage conditions

          -20°C

          Shipping conditions

          Ambient (domestic); Wet ice (international)

          RRID

          AB_2536821

          Product Specific Information

          MA1-110 has been successfully utilized in Western Blot, immunocytochemistry, flow cytometry, and immunofluorescence applications in human samples. MA1-110 also acts a neuronal stem cell marker and detects a band in Western blot at ~250 kDa.

          Target Information

          Nestin is a Class VI intermediate filament abundantly produced in the developing central nervous system (CNS) and in early embryonic neuroepithelial stem cells. Nestin has been widely used as a predominant marker for stem/progenitor cells, glioma cells, and tumor endothelial cells. Also, Nestin is a superior angiogenic marker to evaluate neovascularity of endothelial cells in tumors. Highly specific antibodies to human Nestin can aid in characterizing progenitor cells differentiating into distinct lineages, in enhancing the therapeutic potential of human neural stem/progenitor cells in the treatment of CNS diseases or injury, and in identifying neuroepithelial tumor cells. Nestin expression has been observed in pancreatic hematopoietic stem cell populations, and is considered a novel angiogenesis marker of proliferating endothelial cells in colorectal cancer tissue.

          For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

          Bioinformatics

          Protein Aliases: FLJ21841; intermediate filament protein; Nestin

          View more View less

          Gene Aliases: AA166324; C78523; ESTM46; Nbla00170; NES

          View more View less

          UniProt ID: (Human) P48681, (Mouse) Q6P5H2

          View more View less

          Entrez Gene ID: (Human) 10763, (Mouse) 18008

          View more View less

          Function(s)
          molecular_function structural molecule activity intermediate filament binding protein binding CCR5 chemokine receptor binding
          Process(es)
          multicellular organism development nervous system development negative regulation of neuron apoptotic process cell projection morphogenesis G2/M transition of mitotic cell cycle central nervous system development brain development positive regulation of intermediate filament depolymerization embryonic camera-type eye development negative regulation of protein binding negative regulation of catalytic activity stem cell proliferation positive regulation of neural precursor cell proliferation
          It has to be done as per old AB suggested Products section.
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