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Invitrogen
Nestin Monoclonal Antibody (2Q178)
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FIGURE: 1 / 5
Nestin Antibody (MA1-91657) in ICC/IF
Immunofluorescence analysis of Nestin was performed using 70% confluent log phase PC-12 cells differentiated to neurons. The cells were fixed with 4% paraformaldehyde for 10 Minutes, permeabilized with 0.1% Triton™ X-100 for 10 Minutes, and blocked with 2% BSA for 45 minutes at room temperature. The cells were labeled with Nestin Monoclonal Antibody (2Q178) (Product # MA1-91657) at 10 µg/mL in 0.1% BSA, incubated at 4 degree celsius overnight and then labeled with Donkey anti-Mouse IgG (H+L) Highly Cross-Adsorbed Secondary Antibody, Alexa ... View More
Product Details
MA1-91657
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Dog,
Chicken,
Hamster,
Human,
Mouse,
Rat
Published species
Mouse
Host/Isotype
Mouse
/ IgG1
Class
Monoclonal
Type
Antibody
Clone
2Q178
Immunogen
Nestin purified from embryonic rat spinal cord.
Conjugate
Form
Liquid
Concentration
1 mg/mL
Purification
Protein A, IgG fraction
Storage buffer
TBS
Contains
0.05% sodium azide
Storage conditions
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Shipping conditions
Wet ice
RRID
AB_2151007
Product Specific Information
For use in ICC applications, cells can be fixed with 4% paraformaldehyde buffered with 50mM sodium borate at p.H. 9.5. for IHC-P and IHC-Fr tissue can be fixed with 4% paraformaldehyde at pH 7.4 for light microscopy.
The predicted molecular weight of MA1-91657 is ~200kDa.
Target Information
Nestin is a Class VI intermediate filament abundantly produced in the developing central nervous system (CNS) and in early embryonic neuroepithelial stem cells. Nestin has been widely used as a predominant marker for stem/progenitor cells, glioma cells, and tumor endothelial cells. Also, Nestin is a superior angiogenic marker to evaluate neovascularity of endothelial cells in tumors. Highly specific antibodies to human Nestin can aid in characterizing progenitor cells differentiating into distinct lineages, in enhancing the therapeutic potential of human neural stem/progenitor cells in the treatment of CNS diseases or injury, and in identifying neuroepithelial tumor cells. Nestin expression has been observed in pancreatic hematopoietic stem cell populations, and is considered a novel angiogenesis marker of proliferating endothelial cells in colorectal cancer tissue.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Bioinformatics
Protein Aliases: FLJ21841; intermediate filament protein; Nestin; transitin
Gene Aliases: AA166324; C78523; ESTM46; I79_009594; Nbla00170; NES; paranemin
UniProt ID: (Human) P48681, (Mouse) Q6P5H2
Entrez Gene ID: (Dog) 490410, (Chicken) 395890, (Human) 10763, (Rat) 25491, (Mouse) 18008, (Chinese hamster) 100761741
G2/M transition of mitotic cell cycle
nervous system development
brain development
response to ionizing radiation
neuron differentiation
positive regulation of intermediate filament depolymerization
embryonic camera-type eye development
response to nutrient levels
negative regulation of protein binding
response to drug
negative regulation of catalytic activity
negative regulation of neuron apoptotic process
cell projection morphogenesis
stem cell proliferation
positive regulation of neural precursor cell proliferation
multicellular organism development
central nervous system development
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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