Invitrogen

Nestin Monoclonal Antibody (Rat-401 (Rat401 (4D4))), eBioscience™

Nestin Antibody in Immunocytochemistry (ICC/IF)

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Nestin Antibody (14-5843-82) in ICC/IF

Immunofluorescence analysis of Nestin was performed using 70% confluent log phase PC-12 differentiated to neuronal cells. The cells were fixed with 4% Paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 10 minutes, and blocked with 2% BSA for 45 minutes at room temperature. The cells were labeled with Anti-Nestin Monoclonal Antibody (Rat-401 (Rat401 (4D4))), eBioscience™ (Product # 14-5843-82) at 1:100 dilution in 0.1% BSA, incubated at 4 degree celsius overnight and then labeled with Donkey anti-Mouse IgG (H+L) High... View More

Nestin Antibody in Immunohistochemistry (Paraffin) (IHC (P))

Nestin Antibody in Immunohistochemistry (IHC)

Product Image Gallery

Product Details

14-5843-82

Applications

Tested Dilution

Publications

Western Blot (WB)

0.5 µg/mL

View 2 publications 2 publications

Immunohistochemistry (IHC)

View 2 publications 2 publications

Immunohistochemistry (Paraffin) (IHC (P))

10 µg/mL

View 1 publication 1 publication

Immunohistochemistry (Frozen) (IHC (F))

10 µg/mL

Immunocytochemistry (ICC/IF)

5 µg/mL

View 4 publications 4 publications

Product Specifications

Species Reactivity

Mouse, Rat

Published species

Human, Mouse, Rat

Host/Isotype

Mouse / IgG1

Class

Monoclonal

Type

Antibody

Clone

Rat-401 (Rat401 (4D4))

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

0.5 mg/mL

Purification

Affinity chromatography

Storage buffer

PBS, pH 7.2

Contains

0.09% sodium azide

Storage conditions

4°C

Shipping conditions

Ambient (domestic); Wet ice (international)

AB_1907435

Product Specific Information

Description: The monoclonal antibody Rat-401 recognizes mouse and rat nestin, a Class VI intermediate filament protein (>170 kDa) primarily expressed in neuroepithelial stem cells. Upon neural differentiation, expression is down-regulated and within the adult nervous system is limited to progenitor cells in the cortical subventricular zone, the hippocampal dentate gyrus, dorsal root ganglia satellite cells, and a subpopulation of Schwann cells. Nestin expression is also present in other progenitor cell populations such as pancreatic islet, angiogenic endothelial cells, and bone marrow mesenchymal stem cells. Nestin filaments require vimentin and desmin for assembly into heterodimers and polymers. Upon differentiation, Nestin-containing filaments are replaced by cell type specific intermediate filament proteins, such as GFAP.

Applications Reported: This Rat-401 (Rat401 (4D4)) antibody has been reported for use in western blotting, immunohistochemical staining of frozen tissue sections, immunohistochemical staining of formalin-fixed paraffin embedded tissue sections, and immunocytochemistry.

Applications Tested: This Rat-401 antibody has been tested by immunofluorescent staining of paraformaldehyde fixed and permeabilized cells and by immunohistochemistry of formalin-fixed paraffin embedded mouse tissue using low or high pH antigen retrieval. This can be used at less than or equal to 10 µg/mL. It is recommended that the antibody be titrated for optimal performance in the assay of interest.

Purity: Greater than 90%, as determined by SDS-PAGE.

Aggregation: Less than 10%, as determined by HPLC.

Filtration: 0.2 µm post-manufacturing filtered.

Target Information

Nestin is a Class VI intermediate filament abundantly produced in the developing central nervous system (CNS) and in early embryonic neuroepithelial stem cells. Nestin has been widely used as a predominant marker for stem/progenitor cells, glioma cells, and tumor endothelial cells. Also, Nestin is a superior angiogenic marker to evaluate neovascularity of endothelial cells in tumors. Highly specific antibodies to human Nestin can aid in characterizing progenitor cells differentiating into distinct lineages, in enhancing the therapeutic potential of human neural stem/progenitor cells in the treatment of CNS diseases or injury, and in identifying neuroepithelial tumor cells. Nestin expression has been observed in pancreatic hematopoietic stem cell populations, and is considered a novel angiogenesis marker of proliferating endothelial cells in colorectal cancer tissue.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

RAB35 is required for murine hippocampal development and functions by regulating neuronal cell distribution.

Communications biology

Maejima I,Hara T,Tsukamoto S,Koizumi H,Kawauchi T,Akuzawa T,Hirai R,Kobayashi H,Isobe I,Emoto K,Kosako H,Sato K

14-5843-82 was used in Western Blotting to reveal that RAB35 is required for precise neuronal distribution in the developing hippocampus by regulating the expression of cell adhesion molecules, thereby influencing spatial memory.

Fri Apr 21 00:00:00 UTC 2023

Conductive and injectable hyaluronic acid/gelatin/gold nanorod hydrogels for enhanced surgical translation and bioprinting.

Journal of biomedical materials research. Part A

Kiyotake EA,Thomas EE,Homburg HB,Milton CK,Smitherman AD,Donahue ND,Fung KM,Wilhelm S,Martin MD,Detamore MS

14-5843-82 was used in Immunocytochemistry to develop and characterize a citrate-gold nanorods (GNRs) conductive hydrogel/bioink that provided a refinable and translational platform for future synergistic combination with electric stimulation (ES) to improve functional recovery after spinal cord injury (SCI).

Tue Feb 01 00:00:00 UTC 2022

Identification of potential candidate proteins for reprogramming spinal cord-derived astrocytes into neurons: a proteomic analysis.

Neural regeneration research

Chen WH,Lin YX,Lin L,Zhang BQ,Xu SX,Wang W

14-5843-82 was used in Immunocytochemistry-immunofluorescence to reprogram reactive astrocytes from newborn rat spinal cords into neurons in vitro through the overexpression of NEUROD1.

Mon Nov 01 00:00:00 UTC 2021

Low availability of choline in utero disrupts development and function of the retina.

FASEB journal : official publication of the Federation of American Societies for Experimental Biology

Trujillo-Gonzalez I,Friday WB,Munson CA,Bachleda A,Weiss ER,Alam NM,Sha W,Zeisel SH,Surzenko N

14-5843 was used in Immunohistochemistry-fresh tissue to demonstrate that choline availability plays an essential role in the regulation of temporal progression of retinogenesis and provide evidence for the importance of adequate supply of choline for proper development of the visual system.

Thu Aug 01 00:00:00 UTC 2019

Genetically Corrected iPSC-Derived Neural Stem Cell Grafts Deliver Enzyme Replacement to Affect CNS Disease in Sanfilippo B Mice.

Molecular therapy. Methods & clinical development

Clarke D,Pearse Y,Kan SH,Le SQ,Sanghez V,Cooper JD,Dickson PI,Iacovino M

Published figure using Nestin monoclonal antibody (Product # 14-5843-82) in Immunocytochemistry

Fri Sep 21 00:00:00 UTC 2018

Inflammation-induced miRNA-155 inhibits self-renewal of neural stem cells via suppression of CCAAT/enhancer binding protein β (C/EBPβ) expression.

Scientific reports

Obora K,Onodera Y,Takehara T,Frampton J,Hasei J,Ozaki T,Teramura T,Fukuda K

14-5843 was used in Immunofluorescence to investigate whether miR-155 mediates inflammation-induced suppression of neural stem cell self-renewal.

Mon Feb 27 00:00:00 UTC 2017

Inflammation-induced miRNA-155 inhibits self-renewal of neural stem cells via suppression of CCAAT/enhancer binding protein β (C/EBPβ) expression.

Scientific reports

Obora K,Onodera Y,Takehara T,Frampton J,Hasei J,Ozaki T,Teramura T,Fukuda K

14-5843 was used in Immunofluorescence to investigate whether miR-155 mediates inflammation-induced suppression of neural stem cell self-renewal.

Mon Feb 27 00:00:00 UTC 2017

Inflammation-induced miRNA-155 inhibits self-renewal of neural stem cells via suppression of CCAAT/enhancer binding protein β (C/EBPβ) expression.

Scientific reports

Obora K,Onodera Y,Takehara T,Frampton J,Hasei J,Ozaki T,Teramura T,Fukuda K

14-5843 was used in Immunofluorescence to investigate whether miR-155 mediates inflammation-induced suppression of neural stem cell self-renewal.

Mon Feb 27 00:00:00 UTC 2017

Genetically engineered rat gliomas: PDGF-driven tumor initiation and progression in tv-a transgenic rats recreate key features of human brain cancer.

PloS one

Connolly NP,Stokum JA,Schneider CS,Ozawa T,Xu S,Galisteo R,Castellani RJ,Kim AJ,Simard JM,Winkles JA,Holland EC,Woodworth GF

14584382 was used in immunohistochemistry - paraffin section to develop and characterize a rat model of glioma

Mon Aug 28 00:00:00 UTC 2017

Maturation of embryonic tissues in a lymph node: a new approach for bioengineering complex organs.

Organogenesis

Francipane MG,Lagasse E

14-5843 was used in Immunohistochemistry to investigate whether the lymph node can support early development and maturation from several mid-embryonic mouse tissues including brain, thymus, lung, stomach, and intestine.

Tue Sep 22 00:00:00 UTC 2015

Maturation of embryonic tissues in a lymph node: a new approach for bioengineering complex organs.

Organogenesis

Francipane MG,Lagasse E

Tue Sep 22 00:00:00 UTC 2015

Bioinformatics

Protein Aliases: FLJ21841; intermediate filament protein; Nestin

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Gene Aliases: AA166324; C78523; ESTM46; Nes

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UniProt ID: (Mouse) Q6P5H2

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Entrez Gene ID: (Rat) 25491, (Mouse) 18008

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Function(s)

structural molecule activity intermediate filament binding CCR5 chemokine receptor binding protein binding

Process(es)

G2/M transition of mitotic cell cycle nervous system development brain development response to ionizing radiation neuron differentiation positive regulation of intermediate filament depolymerization embryonic camera-type eye development response to nutrient levels negative regulation of protein binding response to drug negative regulation of catalytic activity negative regulation of neuron apoptotic process cell projection morphogenesis stem cell proliferation positive regulation of neural precursor cell proliferation multicellular organism development

Performance Guarantee

If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

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Nestin Monoclonal Antibody (Rat-401 (Rat401 (4D4))), eBioscience™

Nestin Antibody (14-5843-82) in ICC/IF

Product Details

14-5843-82

Western Blot (WB)

Immunohistochemistry (IHC)

Immunohistochemistry (Paraffin) (IHC (P))

Immunohistochemistry (Frozen) (IHC (F))

Immunocytochemistry (ICC/IF)

Species Reactivity

Published species

Host/Isotype

Class

Type

Clone

Conjugate

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

Shipping conditions

RRID

Product Specific Information

Target Information

Bioinformatics

Performance Guarantee

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