Invitrogen

NeuN Recombinant Rabbit Monoclonal Antibody (14H6L24)

NeuN Antibody in Immunocytochemistry (ICC/IF)

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NeuN Antibody (702022) in ICC/IF

Immunofluorescence analysis of NeuN was performed using 70% confluent log phase SH-SY5Y cells. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 15 minutes and blocked with 2% BSA for 45 minutes at room temperature. The cells were labelled with NeuN Recombinant Rabbit Monoclonal Antibody (14H6L24) (Product # 702022, 5 µg/mL) in 0.1% BSA, incubated at 4 degree Celsius overnight and then labelled with Donkey anti-Rabbit IgG (H+L) Highly Cross-Adsorbed Secondary Antibody, Alexa Fluor™ Plus... View More

NeuN Antibody in Immunohistochemistry (Paraffin) (IHC (P))

NeuN Antibody in Immunoprecipitation (IP)

NeuN Antibody in RNA Immunoprecipitation (RIP)

NeuN Antibody in Immunohistochemistry (IHC)

Product Image Gallery

NeuN Antibody — Antibody specificity was demonstrated by detection of differential basal expression of the target across tissue models owing to their inherent genetic constitution. Relative expression of NeuN was observed in Mouse brain and Rat brain, but not in Mouse heart, Mouse kidney, Rat heart and Rat kidney which are known to be low expressors using NeuN Recombinant Rabbit Monoclonal Antibody (14H6L24) (Product # 702022) in western blot. {RE}

Product Details

702022

Applications

Tested Dilution

Publications

Western Blot (WB)

1-2 µg/mL

View 2 publications 2 publications

Immunohistochemistry (IHC)

View 6 publications 6 publications

Immunocytochemistry (ICC/IF)

3 µg/mL

Immunoprecipitation (IP)

Assay-dependent

RNA Immunoprecipitation (RIP)

Assay-dependent

Product Specifications

Species Reactivity

Human, Mouse, Rat

Published species

Avian, Human, Mouse, Rat

Host/Isotype

Rabbit / IgG

Expression System

Expi293

Class

Recombinant Monoclonal

Type

Antibody

Clone

14H6L24

Immunogen

Protein corresponding to Human RBFOX3 (aa 1-312)

View immunogen

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

0.5 mg/mL

Purification

Protein A

Storage buffer

PBS, pH 7.2

Contains

0.09% sodium azide

Storage conditions

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

Shipping conditions

Wet ice

AB_2633050

Product Specific Information

This antibody is predicted to react with Monkey, Rat and Mouse.

Recombinant rabbit monoclonal antibodies are produced using in vitro expression systems. The expression systems are developed by cloning in the specific antibody DNA sequences from immunoreactive rabbits. Then, individual clones are screened to select the best candidates for production. The advantages of using recombinant rabbit monoclonal antibodies include: better specificity and sensitivity, lot-to-lot consistency, animal origin-free formulations, and broader immunoreactivity to diverse targets due to larger rabbit immune repertoire.

Target Information

Fox-3, also known as NEUN, HRNBP3, RBFOX3 (RNA binding protein, fox-1) or Fox-1 homolog C, is a 350 amino acid protein that contains one RRM (RNA recognition motif) domain. Localized to both the nucleus and cytoplasm, Fox-3 is suggested to regulate alternative splicing events. Fox-3 is encoded by a gene located on human chromosome 17, which is comprised over 2.5% of the human genome and encodes over 1,200 genes. Two key tumor suppressor genes are associated with chromosome 17, namely, p53 and BRCA1. Tumor suppressor p53 is necessary for maintenance of cellular genetic integrity by moderating cell fate through DNA repair versus cell death. Malfunction or loss of p53 expression is associated with malignant cell growth and LiFraumeni syndrome. Like p53, BRCA1 is directly involved in DNA repair, though specifically it is recognized as a genetic determinant of early onset breast cancer and predisposition to cancers of the ovary, colon, prostate gland and fallopian tubes.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

Aberrant crosstalk between insulin signaling and mTOR in young Down syndrome individuals revealed by neuronal-derived extracellular vesicles.

Alzheimer's & dementia : the journal of the Alzheimer's Association

Perluigi M,Picca A,Montanari E,Calvani R,Marini F,Matassa R,Tramutola A,Villani A,Familiari G,Domenico FD,Butterfield DA,Oh KJ,Marzetti E,Valentini D,Barone E

702022 was used in Western Blot to identify the molecular pathways underlying the aging trajectory of infants and adolescents with Down syndrome.

Mon Aug 01 00:00:00 UTC 2022

Aberrant crosstalk between insulin signaling and mTOR in young Down syndrome individuals revealed by neuronal-derived extracellular vesicles.

Alzheimer's & dementia : the journal of the Alzheimer's Association

Perluigi M,Picca A,Montanari E,Calvani R,Marini F,Matassa R,Tramutola A,Villani A,Familiari G,Domenico FD,Butterfield DA,Oh KJ,Marzetti E,Valentini D,Barone E

702022 was used in Western Blot to identify the molecular pathways underlying the aging trajectory of infants and adolescents with Down syndrome.

Mon Aug 01 00:00:00 UTC 2022

Molecular Markers of Mechanosensation in Glycinergic Neurons in the Avian Lumbosacral Spinal Cord.

eNeuro

Stanchak KE,Miller KE,Lumsden EW,Shikiar D,Davis C,Brunton BW,Perkel DJ

702022 was used in Immunohistochemistry to suggest the LSO may be a modification and elaboration of a preexisting mechanosensory spinal network in vertebrates.

Fri Sep 16 00:00:00 UTC 2022

Developmental Regulation of Homeostatic Plasticity in Mouse Primary Visual Cortex.

The Journal of neuroscience : the official journal of the Society for Neuroscience

Wen W,Turrigiano GG

702022 was used in Immunohistochemistry to suggest that homeostatic forms of plasticity can be recruited in a modular manner according to the evolving needs of a developing neural circuit.

Wed Dec 01 00:00:00 UTC 2021

Small molecules efficiently reprogram apical papilla stem cells into neuron-like cells.

Experimental and therapeutic medicine

Chen Q,Yuan C,Jiang S,Heng BC,Zou T,Shen Z,Wang P,Zhang C

702022 was used in Western Blot to study the early phase of small molecule-induced neurogenic differentiation of stem cells from the apical papilla.

Tue Jun 01 00:00:00 UTC 2021

Ultrashort Wave Combined with Human Umbilical Cord Mesenchymal Stem Cell (HUC-MSC) Transplantation Inhibits NLRP3 Inflammasome and Improves Spinal Cord Injury via MK2/TTP Signalling Pathway.

BioMed research international

Na L,Wang S,Liu T,Zhang L

702022 was used in Immunohistochemistry-immunofluorescence to investigate the curative effects of HUC-MSCs and USW on spinal cord injury and the influence MK2/TTP on the inflammatory microenvironment and its effects on spinal cord injury.

Tue Jun 08 00:00:00 UTC 2021

Ultrashort Wave Combined with Human Umbilical Cord Mesenchymal Stem Cell (HUC-MSC) Transplantation Inhibits NLRP3 Inflammasome and Improves Spinal Cord Injury via MK2/TTP Signalling Pathway.

BioMed research international

Na L,Wang S,Liu T,Zhang L

Tue Jun 08 00:00:00 UTC 2021

Fgr contributes to hemorrhage-induced thalamic pain by activating NF-κB/ERK1/2 pathways.

JCI insight

Huang T,Fu G,Gao J,Zhang Y,Cai W,Wu S,Jia S,Xia S,Bachmann T,Bekker A,Tao YX

702022 was used in Immunohistochemistry-immunofluorescence to suggest that Fgr is a key player in thalamic pain and a potential target for the therapeutic management of this disorder.

Thu Oct 15 00:00:00 UTC 2020

Inflammasome Activation Induces Pyroptosis in the Retina Exposed to Ocular Hypertension Injury.

Frontiers in molecular neuroscience

Pronin A,Pham D,An W,Dvoriantchikova G,Reshetnikova G,Qiao J,Kozhekbaeva Z,Reiser AE,Slepak VZ,Shestopalov VI

702022 was used in Immunohistochemistry to map and characterise inflammasome complexes and detect pyroptosis in the ocular hypertension-injured retina.

Wed Sep 30 00:00:00 UTC 2020

Gemfibrozil, a Lipid-Lowering Drug, Lowers Amyloid Plaque Pathology and Enhances Memory in a Mouse Model of Alzheimer's Disease via Peroxisome Proliferator-Activated Receptor α.

Journal of Alzheimer's disease reports

Chandra S,Pahan K

702022 was used in Immunohistochemistry to highlight a new therapeutic property of gemfibrozil and suggest that this drug may be repurposed for treatment of AD.

Sat May 18 00:00:00 UTC 2019

Bioinformatics

Protein Aliases: FLJ56884; FLJ58356; fox 1 homolog (C. elegans) 3; Fox-1 homolog C; Fox-3; fox1 homolog C; FOX3NeuN; hexaribonucleotide binding protein 3; hexaribonucleotide binding protein 3 (HRNBP3); Hexaribonucleotide-binding protein 3; NeuN antigen; neuronal nuclear antigen A60; neuronal nuclei; Neuronal nuclei antigen; RFOX3; RNA binding protein; RNA binding protein fox-1 homolog 3; RNA binding protein, fox-1 homolog (C. elegans) 3; RNA binding protein, fox-1 homolog 3

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Gene Aliases: D11Bwg0517e; FOX-3; FOX3; HRNBP3; NEUN; Neuna60; RBFOX3; RGD1560070

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UniProt ID: (Human) A6NFN3, (Mouse) Q8BIF2

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Entrez Gene ID: (Human) 146713, (Rat) 287847, (Mouse) 52897

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Function(s)

nucleotide binding DNA binding mRNA binding RNA binding nucleic acid binding protein binding

Process(es)

regulation of alternative mRNA splicing, via spliceosome mRNA processing RNA splicing regulation of RNA splicing

Performance Guarantee

If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

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NeuN Recombinant Rabbit Monoclonal Antibody (14H6L24)

NeuN Antibody (702022) in ICC/IF

Product Details

702022

Western Blot (WB)

Immunohistochemistry (IHC)

Immunocytochemistry (ICC/IF)

Immunoprecipitation (IP)

RNA Immunoprecipitation (RIP)

Species Reactivity

Published species

Host/Isotype

Expression System

Class

Type

Clone

Immunogen

Conjugate

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

Shipping conditions

RRID

Product Specific Information

Target Information

Bioinformatics

Performance Guarantee

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