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Neuronal Calcium Sensor 1 Antibody (600-401-J47) in IHC (P)
Immunohistochemistry of Rabbit anti-NCS1 / NCS-1 antibody. Tissue: Brain, cortex. Fixation: formalin fixed paraffin embedded. Antigen retrieval: not required. Primary antibody: NCS1 / NCS-1 antibody at 5 µg/mL for 1 h at RT. Secondary antibody: Peroxidase rabbit secondary antibody at 1:10,000 for 45 min at RT. Staining: NCS1 / NCS-1 as precipitated red signal with hematoxylin purple nuclear counterstain.
Product Details
600-401-J47
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
Neuronal Calcium Sensor 1 affinity purified antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to the C-terminus of human Neuronal Calcium Sensor 1.
Conjugate
Form
Liquid
Concentration
0.42 mg/mL
Purification
Affinity chromatography
Storage buffer
0.02M potassium phosphate, pH 7.2, with 50% glycerol, 0.15M NaCl
Contains
no preservative
Storage conditions
-20°C, Avoid Freeze/Thaw Cycles
Shipping conditions
Ambient (domestic); Wet ice (international)
Product Specific Information
Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
A BLAST analysis was used to suggest cross-reactivity with human, chicken, frog, and rat based on 100% sequence homology. Cross-reactivity with Neuronal Calcium Sensor 1 from other sources has not been determined.
Target Information
This gene is a member of the neuronal calcium sensor gene family, which encode calcium-binding proteins expressed predominantly in neurons. The protein encoded by this gene regulates G protein-coupled receptor phosphorylation in a calcium-dependent manner and can substitute for calmodulin. The protein is associated with secretory granules and modulates synaptic transmission and synaptic plasticity. Multiple transcript variants encoding different isoforms have been found for this gene.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: frequenin; Frequenin homolog; Frequenin-like protein; Frequenin-like ubiquitous protein; NCS-1; Neuronal calcium sensor 1
Gene Aliases: FLUP; FREQ; NCS1
UniProt ID: (Human) P62166
Entrez Gene ID: (Human) 23413
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