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Invitrogen
Neutrophil elastase Polyclonal Antibody
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Neutrophil elastase Antibody (PA5-29659) in WB
Western Blot using Neutrophil elastase Polyclonal Antibody (Product # PA5-29659). Sample (30 µg of whole cell lysate). Lane A: A549. Lane B: HepG2. 12% SDS PAGE. Neutrophil elastase Polyclonal Antibody (Product # PA5-29659) diluted at 1:1,000.
Product Details
PA5-29659
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human
Published species
Human
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
Recombinant protein encompassing a sequence within the center region of human Neutrophil elastase. The exact sequence is proprietary.
Conjugate
Form
Liquid
Concentration
1 mg/mL
Purification
Antigen affinity chromatography
Storage buffer
PBS, pH 7, with 1% BSA, 20% glycerol
Contains
0.01% thimerosal
Storage conditions
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
AB_2547134
Product Specific Information
Recommended positive controls: A549, HepG2.
Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.
Target Information
The neutrophil form of elastase is 218 amino acids long, with two asparagine-linked carbohydrate chains (see glycosylation). It is present in azurophil granules in the neutrophil cytoplasm. There appear to be two forms of neutrophil elastase, termed IIa and IIb.
⚠WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause birth defects or other reproductive harm. For more information go to www.P65Warnings.ca.gov.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Bioinformatics
Protein Aliases: Bone marrow serine protease; ELA2A; Elastase; elastase 2, neutrophil; Elastase-2; Elastase2; granulocyte-derived elastase; HLE; Human leukocyte elastase; leukocyte elastase; Medullasin; Neutrophil elastase; PE-1; PMN elastase; polymorphonuclear elastase
Gene Aliases: ELA2; ELANE; GE; HLE; HNE; NE; PMN-E; SCN1
UniProt ID: (Human) P08246
Entrez Gene ID: (Human) 1991
negative regulation of transcription from RNA polymerase II promoter
response to yeast
acute inflammatory response to antigenic stimulus
proteolysis
cellular calcium ion homeostasis
phagocytosis
response to UV
extracellular matrix disassembly
protein catabolic process
response to lipopolysaccharide
defense response to bacterium
positive regulation of MAP kinase activity
negative regulation of growth of symbiont in host
negative regulation of chemokine biosynthetic process
negative regulation of interleukin-8 biosynthetic process
positive regulation of interleukin-8 biosynthetic process
positive regulation of smooth muscle cell proliferation
negative regulation of inflammatory response
positive regulation of immune response
leukocyte migration
negative regulation of chemotaxis
neutrophil mediated killing of fungus
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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