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Invitrogen
Neutrophil elastase Monoclonal Antibody (ELA10-101.5)
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FIGURE: 1 / 1
Neutrophil elastase Antibody (MA1-10608) in ELISA
PLoS pathogens 2016 -
Fig 2 Flagellum is the main contributor to NET release triggered by P . aeruginosa . (A) Human PMNs were exposed to WT (PAK or PAO1) and flagellum-deficient strains (PAK flgC and PAO1 fliC ) of P . aeruginosa (10 MOI), and the following readouts were measured: (A) Release of myeloperoxidase (MPO) and human neutrophil elastase (HNE) measured by ELISA. MPO: PAK ( n = 5), PAO1 ( n = 4); HNE: PAK ( n = 11), PAO... View More
Product Details
MA1-10608
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human
Host/Isotype
Mouse
/ IgG1
Class
Monoclonal
Type
Antibody
Clone
ELA10-101.5
Immunogen
Purified human Neutrophil Elastase.
Conjugate
Form
Liquid
Concentration
1.4 mg/mL
Purification
Protein G
Storage buffer
PBS, pH 7.4
Contains
no preservative
Storage conditions
-20°C, Avoid Freeze/Thaw Cycles
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
AB_1077224
Product Specific Information
MA1-10608 detects Neutrophil elastase from human samples.
MA1-10608 has been successfully used in ELISA and in studies of inhibition and enzyme kinetics of neutrophil elastase.
The MA1-10608 immunogen is purified human Neutrophil Elastase.
Target Information
The neutrophil form of elastase is 218 amino acids long, with two asparagine-linked carbohydrate chains (see glycosylation). It is present in azurophil granules in the neutrophil cytoplasm. There appear to be two forms of neutrophil elastase, termed IIa and IIb.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Bioinformatics
Protein Aliases: Bone marrow serine protease; ELA2A; Elastase; elastase 2, neutrophil; Elastase-2; Elastase2; granulocyte-derived elastase; HLE; Human leukocyte elastase; leukocyte elastase; Medullasin; Neutrophil elastase; PE-1; PMN elastase; polymorphonuclear elastase
Gene Aliases: ELA2; ELANE; GE; HLE; HNE; NE; PMN-E; SCN1
UniProt ID: (Human) P08246
Entrez Gene ID: (Human) 1991
negative regulation of transcription from RNA polymerase II promoter
response to yeast
acute inflammatory response to antigenic stimulus
proteolysis
cellular calcium ion homeostasis
phagocytosis
response to UV
extracellular matrix disassembly
protein catabolic process
response to lipopolysaccharide
defense response to bacterium
positive regulation of MAP kinase activity
negative regulation of growth of symbiont in host
negative regulation of chemokine biosynthetic process
negative regulation of interleukin-8 biosynthetic process
positive regulation of interleukin-8 biosynthetic process
positive regulation of smooth muscle cell proliferation
negative regulation of inflammatory response
positive regulation of immune response
leukocyte migration
negative regulation of chemotaxis
neutrophil mediated killing of fungus
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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