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Rockland
Ngp Polyclonal Antibody
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FIGURE: 1 / 1
Ngp Antibody (600-401-GW9) in WB
Western Blot of Rabbit anti-Ngp antibody. Lane 1: MW ladder (opal pre-stained) p/n (MB-210-0500). Lane 2: 32D lysate. Load: 10 µg per lane. Primary antibody: Ngp antibody at 1:1000 for overnight at 4°C. Secondary antibody: rabbit secondary HRP antibody (p/n 611-103-122) at 1:70,000 for 45 min at RT. Block: BlockOut (p/n MB-070) overnight at 4°C. Predicted/Observed size: 17, 20 kDa for Ngp. Higher banding due to glycosylation. ~125 kDa non-specific.
Product Details
600-401-GW9
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Mouse
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
Affinity purified Anti-Ngp antibody was prepared from whole rabbit serum produced by repeated immunizations with a recombinant protein of mouse Ngp protein.
Conjugate
Form
Liquid
Concentration
1.1 mg/mL
Purification
Affinity chromatography
Storage buffer
0.02M potassium phosphate, pH 7.2, with 0.15M NaCl
Contains
0.01% sodium azide
Storage conditions
-20°C, Avoid Freeze/Thaw Cycles
Shipping conditions
Wet ice
Product Specific Information
Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
Anti-Ngp is directed against full-length mouse Ngp. A BLAST analysis was used to suggest reactivity with this protein in Ngp based on 100% homology match for the immunogen sequence.
Target Information
A proteomic study in 2011 determined a direct correlation between NGP mRNA levels and myeloid-derived suppressor cells. Specifically cathepsin B, a catalytic mechanism and potentially biomarker in a variety of cancers and amyloid plaque development, was inhibited by NGP. The significance of NGP as a negative regulator of tumor metastasis makes this antibody ideal for investigators involved in cancer research, as well as Alzheimer's research.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: clone B6; Cystatin-like protein; Myeloid bactenecin protein; myeloid granule protein; Myeloid secondary granule protein; Neutrophilic granule protein; NGP
Gene Aliases: bectenecin; Ngp
UniProt ID: (Mouse) O08692
Entrez Gene ID: (Mouse) 18054
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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