Invitrogen

Nhe-1 Polyclonal Antibody

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Datasheet
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Datasheet
Protocols
Questions & Answers

Product Details

PA5-116471

Applications
Tested Dilution
Publications

Western Blot (WB)

1:500-1:1,000
-

Immunohistochemistry (Paraffin) (IHC (P))

1:50-1:200
-

Immunocytochemistry (ICC/IF)

1:50-1:200
-

Flow Cytometry (Flow)

1:50-1:100
-
Product Specifications

Species Reactivity

Human, Mouse, Rat

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

Synthetic peptide within mouse NHE-1 aa 36-85
View immunogen

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

1 mg/mL

Purification

Antigen affinity chromatography

Storage buffer

PBS, pH 7.4, with 50% glycerol, 0.2% BSA

Contains

0.05% sodium azide

Storage conditions

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_2901103

Product Specific Information

Positive controls: Rat skin lysates, A431, 293T, LOVO, human kidney tissue, mouse colon tissue, human small intestine tissue.

Target Information

The Na+/H+ antiporter (Nhe-1) is a ubiquitous membrane-bound enzyme involved in pH regulation of vertebrate cells and is specifically inhibited by the diuretic drug amiloride and activated by a variety of signals including growth factors, mitogens, neurotransmitters, and tumor promoters. Nhe-1 acts as an anchor for actin filaments to control the integrity of the cortical cytoskeleton. This occurs through a previously unrecognized structural link between Nhe-1 and the actin-binding proteins ezrin, radixin, and moesin, collectively referred to as ERM proteins. A structural role for Nhe-1 has been proposed in regulating the cortical cytoskeleton that is independent of its function as an ion exchanger. It is also thought that Nhe-1 play a role in hypertension. At least two isoforms of Nhe-1 are known to exist.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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Bioinformatics

Protein Aliases: APNH; Na(+)/H(+) antiporter, amiloride-sensitive; Na(+)/H(+) exchanger 1; Na+/H+ antiporter; Na+/H+, amiloride sensitive; Na-Li countertransporter; NHE-1; protein phosphatase 1, regulatory subunit 143; slow-wave epilepsy; Sodium/hydrogen exchanger 1; Solute carrier family 9 (sodium/hydrogen exchanger 1), antiporter, Na+/H+, (amiloride sensitive); solute carrier family 9 (sodium/hydrogen exchanger), isoform 1 (antiporter, Na+/H+, amiloride sensitive); solute carrier family 9 (sodium/hydrogen exchanger), member 1; solute carrier family 9 (sodium/hydrogen exchanger), member 1 (antiporter, Na+/H+, amiloride sensitive); Solute carrier family 9 member 1; solute carrier family 9, member 1; solute carrier family 9, subfamily A (NHE1, cation proton antiporter 1), member 1

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Gene Aliases: APNH; APNH1; AW554487; LIKNS; mir-5122; Mir5122; NHE-1; NHE1; PPP1R143; SLC9A1; swe

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UniProt ID: (Human) P19634, (Rat) P26431, (Mouse) Q61165

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Entrez Gene ID: (Human) 6548, (Rat) 24782, (Mouse) 20544

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Function(s)
protein binding calmodulin binding phosphatidylinositol-4,5-bisphosphate binding solute:proton antiporter activity sodium:proton antiporter activity potassium:proton antiporter activity protein phosphatase 2B binding protein binding, bridging protein complex scaffold calcium-dependent protein binding sodium:proton antiporter activity involved in regulation of cardiac muscle cell membrane potential antiporter activity
Process(es)
ion transport cellular sodium ion homeostasis regulation of pH response to acidic pH positive regulation of cardiac muscle hypertrophy regulation of cardiac muscle contraction by calcium ion signaling cell growth cell migration maintenance of cell polarity hyaluronan catabolic process positive regulation of cell growth cellular response to insulin stimulus positive regulation of mitochondrial membrane permeability response to muscle stretch response to drug positive regulation of apoptotic process negative regulation of apoptotic process positive regulation of action potential positive regulation of transcription from RNA polymerase II promoter protein oligomerization regulation of intracellular pH regulation of stress fiber assembly positive regulation of NFAT protein import into nucleus regulation of focal adhesion assembly regulation of sensory perception of pain cardiac muscle cell differentiation positive regulation of calcineurin-NFAT signaling cascade neuron death cellular response to antibiotic cellular response to electrical stimulus cellular response to mechanical stimulus sodium ion export cellular response to hypoxia cellular response to acidic pH potassium ion transmembrane transport cellular response to epinephrine stimulus cardiac muscle cell contraction regulation of cardiac muscle cell membrane potential regulation of the force of heart contraction by cardiac conduction sodium ion import across plasma membrane positive regulation of the force of heart contraction hydrogen ion transmembrane transport positive regulation of calcium:sodium antiporter activity regulation of the force of heart contraction sodium ion transport response to organic cyclic compound cell differentiation sodium ion transmembrane transport cellular response to organic cyclic compound positive regulation of intracellular signal transduction transport cation transport embryo development transmembrane transport
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