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Invitrogen
Nidogen G3 Domain Monoclonal Antibody (JF2)
Product Details
MA5-17020
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse
Host/Isotype
Rat
/ IgG2a
Class
Monoclonal
Type
Antibody
Clone
JF2
Immunogen
Recombinant fragment corresponding to the G3 region of mouse nidogen produced in Fisher rat plus YB2/0 cells
Conjugate
Form
Liquid
Concentration
1 mg/mL
Purification
Protein G
Storage buffer
2M tris, 0.5M acetic acid, tissue culture supernatant
Contains
0.1% sodium azide
Storage conditions
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
AB_2538492
Product Specific Information
This antibody recognizes native and denatured mouse and human nidogen, and blocks binding of nidogen to laminin.
It has been successfully used in ELISA and Western blot applications.
The immunogen is a recombinant fragment corresponding to the G3 region of mouse nidogen produced in Fisher rat plus YB2/0 cells.
Target Information
Basement membrane components play an important role in cell adhesion, locomotion, differentiation and embryonic compartmentalization. Although the precise composition and structure of basal laminae varies from tissue to tissue, as well as within the same tissue at different developmental stages, most mature basal laminae contain type IV collagen, laminins, heparan sulphate proteoglycans and entactin (or nidogen). Entactin is a sulfated glycoprotein that is widely distributed in basement membranes. It is tightly associated with laminin and also binds to collagen IV. Entactin probably has a role in cell-extracellular matrix interactions.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: enactin; Ent1; Entactin; entactin 1; NID 1; NID-1; Nidogen (enactin); Nidogen precursor (Entactin); Nidogen-1
Gene Aliases: A630025O17; Ent; entactin; entactin-1; NID; NID1; nidogen-1
UniProt ID: (Human) P14543, (Mouse) P10493
Entrez Gene ID: (Human) 4811, (Mouse) 18073
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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