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Bethyl Laboratories
Ninein Polyclonal Antibody
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Ninein Antibody (A301-504A) in WB
Detection of human and mouse NINEIN by western blot (h&m) and immunoprecipitation (h). Samples: Whole cell lysate from HeLa (5, 15 and 50 µg for WB; 1 mg for IP, 20% of IP loaded), HEK293T (T; 50 µg) and mouse NIH 3T3 (M; 50 µg)cells. Antibodies: Affinity purified rabbit anti-NINEIN antibody (Product # A301-504A) used for WB at 0.04 µg/mL (A) and 1 µg/mL (B) and used for IP at 3 µg/mg lysate. NINEIN was also immunoprecipitated by rabbit anti-NINEIN antibody BL5899, which recognizes an upstream epitope. Detection: Chemiluminescence with exp... View More
Product Details
A301-504A
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
Region between residue 1850 and 1900 of human Ninein.
Conjugate
Form
Liquid
Concentration
0.20 mg/mL
Purification
Antigen affinity chromatography
Storage buffer
TBS, pH 7.0 to 8.0, with 0.1% BSA
Contains
0.09% sodium azide
Storage conditions
4°C
Shipping conditions
Ambient (domestic); Wet ice (international)
Product Specific Information
The recommended shelf life for this product is 1 year from date of receipt.
Target Information
Ninein is a component of subdistal appendages of the mother centriole, localized specifically in the pericentriolar matrix of the centrosome. It may be involved in microtubule minus-end capping, centriole positioning, and protein anchoring. Recent studies have shown that the microtubule nucleation and anchoring at the centrosome are independent processes linked by ninein function. At least five human ninein isoforms with divergent C-terminal domains and two mouse isoforms have been reported. Data indicates that the combined action of domains (C-terminal and N-terminal) might, in the absence of coiled coil region, be sufficient to localize ninein to the mother centriole. Microinjection of antibodies against ninein into metaphase HeLa cells can disrupt the reformation of tubular conformation of proteins within the centrosome following cell division, and consequently lead to dispersal of centrosomal material throughout the cytosol. MTOC (microtubule organizing center) function can be disrupted when anti-ninein antibodies are injected into postmitotic cells. Antibodies against ninein are present in sera from patients with autoimmune diseases that develop autoantibodies to centrosomal proteins.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: Glycogen synthase kinase 3 beta-interacting protein; GSK3B-interacting protein; hNinein; Ninein; ninein (GSK3B interacting protein); ninein centrosomal protein
Gene Aliases: 3110068G20Rik; AI385615; AU024711; KIAA1565; mKIAA1565; NIN; SCKL7
UniProt ID: (Human) Q5BKU3, (Mouse) Q61043
Entrez Gene ID: (Human) 51199, (Mouse) 18080
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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