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          • Primary Antibodies ›
          • Nitrotyrosine Antibodies

          Invitrogen

          Nitrotyrosine Monoclonal Antibody (HM11)

          6 Published Figures
          25 References
          View all (11) Nitrotyrosine antibodies
          Datasheet
          Protocols
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          Datasheet
          Protocols
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          Cite Nitrotyrosine Monoclonal Antibody (HM11)

          • Published Figures (6)
          Nitrotyrosine Antibody in Immunohistochemistry (Paraffin) (IHC (P))
          Group 53 Created with Sketch.
          Nitrotyrosine Antibody in Immunohistochemistry (Paraffin) (IHC (P))
          Group 53 Created with Sketch.

          FIGURE: 1 / 6

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          Nitrotyrosine Antibody (32-1900) in IHC (P)

          Histochemistry and cell biology 2012 - Fig. 2 a Levels of total IPT in the serum of rats in IH and Nx for 3 (D3) and 7 days (D7). * p < 0.05 versus corresponding Nx ( n = 4 for each group). b Protein expression of NTR in the CB of rats in IH and Nx for 3 (D3) and 7 days (D7). Data are presented in % positive immunostains with positive staining of NTR immunoreactivities. * p < 0.0005 versus corresponding Nx ( n = 4 for each group). c Immu... View More {{ $ctrl.currentElement.advancedVerification.fullName }} validation info. View more
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          View Product

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          Nitrotyrosine Antibody in Immunohistochemistry (Paraffin) (IHC (P))
          Nitrotyrosine Antibody in Immunohistochemistry (Paraffin) (IHC (P))
          Nitrotyrosine Antibody in Western Blot (WB)
          Nitrotyrosine Antibody in Western Blot (WB)
          Nitrotyrosine Antibody in Western Blot (WB)
          Nitrotyrosine Antibody in Western Blot (WB)

          Product Details

          32-1900

          Applications
          Tested Dilution
          Publications

          Western Blot (WB)

          1-2 µg/mL
          View 11 publications 11 publications

          Immunohistochemistry (IHC)

          1-2 µg/mL
          View 4 publications 4 publications

          Immunohistochemistry (Paraffin) (IHC (P))

          -
          View 5 publications 5 publications

          ELISA (ELISA)

          -
          View 2 publications 2 publications

          Miscellaneous PubMed (MISC)

          -
          View 3 publications 3 publications
          Product Specifications

          Species Reactivity

          Chemical

          Published species

          Chemical

          Host/Isotype

          Mouse / IgG1

          Class

          Monoclonal

          Type

          Antibody

          Clone

          HM11

          Immunogen

          Nitrotyrosine attached to a carrier protein

          Conjugate

          Unconjugated Unconjugated Unconjugated

          Form

          Liquid

          Concentration

          0.5 mg/mL

          Purification

          Protein G

          Storage buffer

          PBS, pH 7.4

          Contains

          0.1% sodium azide

          Storage conditions

          -20°C

          Shipping conditions

          Ambient (domestic); Wet ice (international)

          RRID

          AB_2533069

          Target Information

          Nitrotyrosine is a relatively stable product formed from various reaction pathways. Perhaps most notable is the reaction of peroxynitrite (formed from superoxide and nitric oxide (NO) radicals) with tyrosine. As a strong oxidant and nitrating agent, peroxynitrite mediates tyrosine nitration reactions on proteins resulting in inactivation of certain housekeeping enzymes (e. g., alpha1-antiproteinase) as well as endogenous antioxidant enzymes such as catalase and superoxide dismutase (SOD). Nitrotyrosine has been identified as an indicator of cell damage and inflammation, as well as of the production of NO. It is believed that measuring the concentration of nitrotyrosine will serve as a marker for damage caused by NO in the cell. Nitrotyrosine has been implicated in the pathogenesis of several inflammatory, infectious, and degenerative human diseases, such as Alzheimer's and Parkinson's disease, multiple sclerosis, amyotrophic lateral sclerosis (ALS), asthma, atherosclerosis, and a variety of conditions precipitated by endothelial injury. At the protein level, tyrosine nitration can lead to loss or alteration of protein function, as demonstrated for the oncogenic protein p53 and the mitochondrial protein, manganese-dependent superoxide dismutase (MnSOD).

          For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

          It has to be done as per old AB suggested Products section.
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