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Nkx2.2 Antibody (PA5-85439) in WB
Western blot analysis of Nkx2.2 in non-transfected (–) and transfected (+) 293T whole cell extracts (30 µg). Samples were incubated with Nkx2.2 polyclonal antibody (Product # PA5-85439) using a dilution of 1:5,000 followed by HRP-conjugated anti-rabbit IgG. Separated by 12% SDS-PAGE.
Product Details
PA5-85439
Product Specifications
Species Reactivity
Human
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
Recombinant protein encompassing a sequence within the center region of human Nkx2.2. The exact sequence is proprietary.
Conjugate
Form
Liquid
Concentration
0.79 mg/mL
Purification
Antigen affinity chromatography
Storage buffer
0.1M tris glycine, pH 7, with 20% glycerol
Contains
0.01% thimerosal
Storage conditions
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Shipping conditions
Wet ice
RRID
AB_2792579
Product Specific Information
Keep as concentrated solution.
Predicted reactivity: Mouse (99%), Rat (99%), Xenopus laevis (88%), Chicken (95%), Bovine (100%).
Positive Control: Nkx2.2-transfected 293T.
Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.
Target Information
The protein encoded by this gene contains a homeobox domain and may be involved in the morphogenesis of the central nervous system. This gene is found on chromosome 20 near NKX2-4, and these two genes appear to be duplicated on chromosome 14 in the form of TITF1 and NKX2-8. The encoded protein is likely to be a nuclear transcription factor.
⚠WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause birth defects or other reproductive harm. For more information go to www.P65Warnings.ca.gov.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: Homeobox protein NK-2 homolog B; Homeobox protein Nkx-2.2; NK2 transcription factor related, locus 2; NK2 transcription factor-like protein B; Nkx2.2
Gene Aliases: NKX2-2; NKX2.2; NKX2B
UniProt ID: (Human) O95096
Entrez Gene ID: (Human) 4821
type B pancreatic cell development
pancreatic A cell fate commitment
type B pancreatic cell fate commitment
pancreatic PP cell fate commitment
transcription, DNA-templated
smoothened signaling pathway
brain development
response to glucose
oligodendrocyte development
spinal cord motor neuron differentiation
spinal cord oligodendrocyte cell fate specification
optic nerve development
endocrine pancreas development
response to progesterone
negative regulation of neuron differentiation
positive regulation of neuron differentiation
positive regulation of transcription from RNA polymerase II promoter
digestive tract development
neuron fate specification
astrocyte differentiation
positive regulation of oligodendrocyte differentiation
positive regulation of sequence-specific DNA binding transcription factor activity
ventral spinal cord interneuron fate determination
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