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Invitrogen
Nkx3.1 Monoclonal Antibody (3 9)
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Nkx3.1 Antibody (35-9700) in WB, ICC/IF
F1000Research 2016 -
Figure S1. Transfection of FLAG-NKX3.1 expression into LNCaP cells and affinity purification. ( A ) LNCaP cells were transfected with pFLAG-NKX3.1 plasmid or with the empty pFLAG vector. Total cell lysate (lanes 1 and 2) was absorbed to anti-FLAG resin and eluted with FLAG peptides (lanes 5 and 6). The depleted cell lysate after affinity purification is shown in lanes 3 and 4. Immunoblots were probed with t... View More
Product Details
35-9700
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse
Published species
Human
Host/Isotype
Mouse
/ IgG1
Class
Monoclonal
Type
Antibody
Clone
3 9
Immunogen
Fusion protein containing the N-terminal domain of the human Nkx3.1 protein.
Conjugate
Form
Liquid
Concentration
0.5 mg/mL
Purification
Protein A
Storage buffer
PBS, pH 7.4
Contains
0.1% sodium azide
Storage conditions
-20°C
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
AB_2533232
Target Information
The homeodomain-containing transcription factor NKX3-1 is a putative prostate tumor suppressor that is expressed in a largely prostate-specific and androgen-regulated manner. Loss of NKX3-1 protein expression is a common finding in human prostate carcinomas and prostatic intraepithelial neoplasia.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Bioinformatics
Protein Aliases: Drosophila NK-3 transcription factor, locus 1; Homeobox protein NK-3 homolog A; Homeobox protein Nkx-3.1; NK homeobox, family 3, A; NK3 transcription factor homolog A; NK3 transcription factor related, locus 1; NKX3-1 antibody; prostate-specific ag
Gene Aliases: bagpipe; BAPX2; Bax; Nkx-3.1; NKX3; NKX3-1; NKX3.1; NKX3A
UniProt ID: (Human) Q99801, (Mouse) P97436
Entrez Gene ID: (Human) 4824, (Mouse) 18095
transcription regulatory region sequence-specific DNA binding
transcription factor activity, RNA polymerase II core promoter sequence-specific
core promoter binding
transcription factor activity, sequence-specific DNA binding
androgen receptor activity
protein binding
transcription factor binding
cysteine-type endopeptidase activator activity involved in apoptotic process
estrogen receptor activity
protein kinase activator activity
estrogen receptor binding
histone deacetylase binding
sequence-specific DNA binding
protein self-association
transcription regulatory region DNA binding
MADS box domain binding
DNA binding
urogenital system development
positive regulation of protein phosphorylation
transcription, DNA-templated
regulation of transcription, DNA-templated
transcription from RNA polymerase II promoter
activation of cysteine-type endopeptidase activity involved in apoptotic process
multicellular organism development
salivary gland development
positive regulation of cell proliferation
negative regulation of cell proliferation
positive regulation of gene expression
negative regulation of gene expression
positive regulation of cell death
positive regulation of phosphatidylinositol 3-kinase signaling
androgen receptor signaling pathway
prostate gland development
response to testosterone
internal genitalia morphogenesis
positive regulation of cysteine-type endopeptidase activity involved in apoptotic process
steroid hormone mediated signaling pathway
protein kinase B signaling
negative regulation of insulin-like growth factor receptor signaling pathway
positive regulation of protein kinase activity
negative regulation of transcription, DNA-templated
positive regulation of transcription, DNA-templated
negative regulation of mitotic cell cycle
positive regulation of mitotic cell cycle
positive regulation of transcription from RNA polymerase II promoter
branching morphogenesis of an epithelial tube
negative regulation of epithelial cell proliferation
positive regulation of cell division
branching involved in prostate gland morphogenesis
epithelial cell proliferation involved in salivary gland morphogenesis
negative regulation of epithelial cell proliferation involved in prostate gland development
cellular response to steroid hormone stimulus
cellular response to hypoxia
mitotic cell cycle arrest
negative regulation of estrogen receptor binding
positive regulation of androgen secretion
positive regulation of response to DNA damage stimulus
positive regulation of apoptotic signaling pathway
positive regulation of intrinsic apoptotic signaling pathway
metanephros development
somitogenesis
heart development
male gonad development
activation of protein kinase activity
cellular response to drug
dorsal aorta development
pharyngeal system development
cellular response to interleukin-1
cellular response to tumor necrosis factor
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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