Invitrogen

Nod2 Monoclonal Antibody (26mNOD2), eBioscience™

2 Published Figures
7 References
View all (16) NOD2 antibodies

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Datasheet
Protocols
Questions & Answers
Datasheet
Protocols
Questions & Answers

Product Details

14-5858-82

Applications
Tested Dilution
Publications

Western Blot (WB)

Assay-Dependent
View 5 publications 5 publications

Immunohistochemistry (IHC)

-
View 1 publication 1 publication

Miscellaneous PubMed (MISC)

-
View 1 publication 1 publication
Product Specifications

Species Reactivity

Mouse

Published species

Mouse

Host/Isotype

Rat / IgG1, kappa

Class

Monoclonal

Type

Antibody

Clone

26mNOD2

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

0.5 mg/mL

Purification

Affinity chromatography

Storage buffer

PBS, pH 7.2

Contains

0.09% sodium azide

Storage conditions

4°C

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_1107022

Product Specific Information

Description: The monoclonal antibody 26mNOD2 reacts with mouse NOD2. NOD2, also referred to as caspase recruitment domain-containing protein 15 (CARD 15), is a cytosolic protein of approximately 115kDa. NOD2 contains a central NOD (nucleotide oligomerization domain) that is flanked by a C-terminal leucine-rich repeat (LRR) domain and an N-terminal CARD domain. The LRR confers recognition of microbial components such as muramyl dipeptide and the CARD domain confers the ability to interact with RIP2 via homotypic interaction with the RIP2 CARD. This interaction leads to downstream activation of NF-kappaB.

The antibody 26mNOD2 has been shown to recognize protein from transgenic and transfected cells. Refer to references for expression in endogenous cells.

Applications Reported: This 26mNOD2 antibody has been reported for use in immunoblotting (WB).

Applications Tested: This 26mNOD2 antibody has been tested by immunoblotting of lysates of 293T cells transfected with mouse NOD2. This antibody has been validated on transfected cell lines and cells from NOD2-transgenic mice.

Purity: Greater than 90%, as determined by SDS-PAGE.

Aggregation: Less than 10%, as determined by HPLC.

Filtration: 0.2 µm post-manufacturing filtered.

Target Information

NOD2 is a member of the apoptosis regulating protein family that includes caspase recruitment-domains, as well as Apaf-1 and NOD1. It contains two N-terminal CARDs, a nucleotide binding domain (NBD), and multiple C-terminal leucine-rich repeats (LRRs). NOD2 is expressed in monocytes (whereas NOD1 is expressed in multiple tissues). NOD2 plays a role in regulating NF-kappaB, acts as an intracellular receptor for bacterial lipopolysaccharides, and contributes to inflammatory bowel disease (IBD) and Crohn's disease.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Bioinformatics

Protein Aliases: caspase recruitment domain family, member 15; caspase recruitment domain-containing protein 15; NOD 2; NOD-2; NOD2 protein; nucleotide-binding oligomerization domain-containing protein 2

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Gene Aliases: ACUG; BLAU; Card15; CD; F830032C23Rik; IBD1; Nlrc2

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Entrez Gene ID: (Mouse) 257632

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Function(s)
nucleotide binding protein binding ATP binding endodeoxyribonuclease activity, producing 5'-phosphomonoesters enzyme binding protein kinase binding muramyl dipeptide binding peptidoglycan binding CARD domain binding
Process(es)
innate immune response in mucosa activation of immune response cytokine production involved in immune response cytokine secretion involved in immune response immune system process immunoglobulin production involved in immunoglobulin mediated immune response positive regulation of dendritic cell antigen processing and presentation negative regulation of T cell mediated immunity positive regulation of dendritic cell cytokine production positive regulation of type 2 immune response negative regulation of inflammatory response to antigenic stimulus positive regulation of humoral immune response mediated by circulating immunoglobulin defense response inflammatory response positive regulation of antibacterial peptide biosynthetic process positive regulation of biosynthetic process of antibacterial peptides active against Gram-positive bacteria signal transduction positive regulation of cell proliferation detection of biotic stimulus detection of bacterium maintenance of gastrointestinal epithelium positive regulation of protein ubiquitination negative regulation of NF-kappaB transcription factor activity response to peptidoglycan response to muramyl dipeptide response to lipopolysaccharide detection of muramyl dipeptide negative regulation of interferon-gamma production negative regulation of interleukin-12 production negative regulation of interleukin-18 production negative regulation of interleukin-2 production negative regulation of tumor necrosis factor production positive regulation of interleukin-1 beta production positive regulation of interleukin-10 production positive regulation of interleukin-12 production positive regulation of interleukin-17 production positive regulation of interleukin-6 production positive regulation of tumor necrosis factor production positive regulation of stress-activated MAPK cascade negative regulation of toll-like receptor 2 signaling pathway activation of MAPK activity involved in innate immune response intracellular signal transduction defense response to bacterium regulation of apoptotic process positive regulation of I-kappaB kinase/NF-kappaB signaling response to exogenous dsRNA positive regulation of MAPK cascade positive regulation of phosphatidylinositol 3-kinase activity negative regulation of growth of symbiont in host innate immune response positive regulation of innate immune response positive regulation of Notch signaling pathway positive regulation of transcription from RNA polymerase II promoter positive regulation of JNK cascade positive regulation of epithelial cell proliferation positive regulation of interleukin-1 beta secretion regulation of inflammatory response positive regulation of peptidyl-tyrosine phosphorylation positive regulation of phagocytosis defense response to Gram-positive bacterium positive regulation of B cell activation positive regulation of NF-kappaB transcription factor activity protein oligomerization positive regulation of oxidoreductase activity positive regulation of nitric-oxide synthase biosynthetic process positive regulation of prostaglandin-endoperoxide synthase activity positive regulation of ERK1 and ERK2 cascade nucleotide-binding oligomerization domain containing 2 signaling pathway cellular response to peptidoglycan cellular response to muramyl dipeptide macrophage inflammatory protein-1 alpha production regulation of neutrophil chemotaxis positive regulation of cytokine production involved in inflammatory response negative regulation of macrophage apoptotic process positive regulation of prostaglandin-E synthase activity
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