Invitrogen

Nodal Polyclonal Antibody

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Datasheet
Protocols
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Datasheet
Protocols
Questions & Answers

Product Details

PA5-28486

Applications
Tested Dilution
Publications

Western Blot (WB)

1:500-1:3,000
-

Immunohistochemistry (Paraffin) (IHC (P))

1:100-1:1,000
-

ELISA (ELISA)

1:1,000-1:10,000
-
Product Specifications

Species Reactivity

Human, Mouse, Rat

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

Recombinant protein encompassing a sequence within the center region of human Nodal. The exact sequence is proprietary.

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

1 mg/mL

Purification

Antigen affinity chromatography

Storage buffer

0.1M tris glycine, pH 7, with 10% glycerol

Contains

0.01% thimerosal

Storage conditions

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_2545962

Product Specific Information

Recommended positive controls: Human ESC.

Predicted reactivity: Mouse (91%), Rat (92%), Bovine (93%).

Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.

Target Information

NODAL is an important TGF-beta family protein in cellular differentiation. While constitutively expressed in many developing tissues, NODAL is problematically associated with the differentiation and development of many cancers. The expression of NODAL is regulated by a Notch signaling pathway, which when disrupted contributes to the aggressive behavior of metastatic cancer cells, particularly in melanoma. The abundance of NODAL expression in melanoma can help determine the stage of cancer and metastatic type/progression when tested in IHC. NODAL interactions with Cripto also predispose toward tumor progression. Overexpression of NODAL is also associated with preeclamptic placenta.

⚠WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause birth defects or other reproductive harm. For more information go to www.P65Warnings.ca.gov.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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Bioinformatics

Protein Aliases: early embryo mesoderm formation; MGC138230; Nodal; Nodal homolog; transgenic lethal 413.d

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Gene Aliases: HTX5; NODAL; Tg.413d

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UniProt ID: (Human) Q96S42, (Mouse) P43021

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Entrez Gene ID: (Human) 4838, (Rat) 294503, (Mouse) 18119

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Function(s)
cytokine activity transforming growth factor beta receptor binding growth factor activity morphogen activity receptor agonist activity type I activin receptor binding protein binding
Process(es)
negative regulation of transcription from RNA polymerase II promoter in utero embryonic development gastrulation with mouth forming second mesoderm formation trophectodermal cell differentiation trophectodermal cellular morphogenesis neural fold formation liver development placenta development embryonic placenta development maternal placenta development vasculature development heart looping inhibition of neuroepithelial cell differentiation determination of left/right symmetry gastrulation nervous system development brain development endoderm development heart development positive regulation of cell proliferation embryonic pattern specification anterior/posterior axis specification anterior/posterior pattern specification regulation of signal transduction polarity specification of proximal/distal axis regulation of gastrulation positive regulation of vascular endothelial growth factor production negative regulation of cell development positive regulation of pathway-restricted SMAD protein phosphorylation cell migration stem cell population maintenance positive regulation of cell-cell adhesion lung development positive regulation of activin receptor signaling pathway floor plate morphogenesis embryonic heart tube development endodermal cell differentiation nodal signaling pathway growth cell migration involved in gastrulation regulation of apoptotic process positive regulation of cysteine-type endopeptidase activity involved in apoptotic process regulation of MAPK cascade cell fate commitment negative regulation of cell differentiation positive regulation of angiogenesis positive regulation of transcription from RNA polymerase II promoter axial mesodermal cell fate specification mesendoderm development cell development digestive tract morphogenesis anatomical structure formation involved in morphogenesis embryonic cranial skeleton morphogenesis tissue morphogenesis formation of anatomical boundary positive regulation of epithelial cell proliferation positive regulation of sequence-specific DNA binding transcription factor activity digestive system development embryonic process involved in female pregnancy maternal process involved in parturition positive regulation of SMAD protein import into nucleus SMAD protein signal transduction left lung morphogenesis negative regulation of androgen receptor signaling pathway epiblast cell-extraembryonic ectoderm cell signaling involved in anterior/posterior axis specification positive regulation of ERK1 and ERK2 cascade primitive streak formation transforming growth factor beta receptor signaling pathway involved in primitive streak formation nodal signaling pathway involved in determination of lateral mesoderm left/right asymmetry negative regulation of trophoblast cell migration negative regulation of chorionic trophoblast cell proliferation positive regulation of receptor activity BMP signaling pathway positive regulation of nodal signaling pathway involved in determination of lateral mesoderm left/right asymmetry regulation of stem cell population maintenance transforming growth factor beta receptor signaling pathway multicellular organism development
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