Invitrogen

Nrf2 Polyclonal Antibody

Nrf2 Antibody in Immunocytochemistry (ICC/IF)

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Nrf2 Antibody (PA5-27882) in ICC/IF

Immunofluorescence analysis of Nuclear factor erythroid 2-related factor 2 was performed using 70% confluent log phase Hep G2 cells treated with TBHQ (100 micromolar for 4h). The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 15 minutes, and blocked with 2% BSA for 45 minutes at room temperature. The cells were labeled with Nrf2 Polyclonal Antibody (Product # PA5-27882) at 1:100 dilution in 0.1% BSA, incubated at 4-degree Celsius overnight, and then labeled with Donkey anti-Rabbit IgG (H... View More

Nrf2 Antibody in Immunohistochemistry (Paraffin) (IHC (P))

Nrf2 Antibody in Immunoprecipitation (IP)

Nrf2 Antibody in Western Blot, Immunohistochemistry (WB, IHC)

Nrf2 Antibody in Western Blot, Immunoprecipitation (WB, IP)

Product Image Gallery

Nrf2 Antibody — Antibody specificity was demonstrated by siRNA mediated knockdown of target protein. U-2 OS cells were transfected with Nuclear factor erythroid 2-related factor 2 siRNA and decrease in signal intensity was observed in Western Blot application using Anti-Nrf2 Polyclonal Antibody (Product # PA5-27882). {KD}

Product Details

PA5-27882

Applications

Tested Dilution

Publications

Western Blot (WB)

1:500-1:3,000

View 20 publications 20 publications

Immunohistochemistry (IHC)

View 4 publications 4 publications

Immunohistochemistry (Paraffin) (IHC (P))

1:100-1:1,000

Immunocytochemistry (ICC/IF)

1:100-1:1,000

View 4 publications 4 publications

Flow Cytometry (Flow)

Assay-dependent

Immunoprecipitation (IP)

1:100-1:500

View 1 publication 1 publication

ChIP assay (ChIP)

5 µg

View 1 publication 1 publication

Miscellaneous PubMed (MISC)

View 1 publication 1 publication

Product Specifications

Species Reactivity

Avian, Human, Mouse, Plant, Reptile, Rat, Zebrafish

Published species

Human, Mouse, Pig, Rat, Zebrafish

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

Recombinant protein encompassing a sequence within the center region of human NRF2. The exact sequence is proprietary.

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

0.65 mg/mL

Purification

Antigen affinity chromatography

Storage buffer

PBS, pH 7, with 20% glycerol

Contains

0.025% ProClin 300

Storage conditions

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

Shipping conditions

Ambient (domestic); Wet ice (international)

AB_2545358

Product Specific Information

Recommended positive controls: C8D30, 3xFlag-human NFE2L2-transfected 293T, MDA-MB-231 nuclear extract, MDA-MB-231 nuclear extract (30 µM tBHQ, 4hr), HepG2 (10 µM MG132 treatment for 15 hr).

Predicted reactivity: Rhesus Monkey (99%), Bovine (84%).

Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.

Target Information

Nfr1 (also designated TCF11), Nrf2 and Nfr3 are bZIP transcription factors that heterodimer with Maf proteins to bind MARE (Maf recognition elements) sequences. Nrf2 is widely expressed and is thought to translocate to the nucleus after treatment with xenobiotics and antioxidants, which stimulate its release from a repressor protein Keap1.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

Urolithin C alleviates pancreatic β-cell dysfunction in type 1 diabetes by activating Nrf2 signaling.

Nutrition & diabetes

Luo C,Hou C,Yang D,Tan T,Chao C

PA5-27882 was used in Immunocytochemistry to explore the mechanism underlying urolithin C (UC)-mediated regulation of β-cell function.

Fri Dec 01 00:00:00 UTC 2023

Urolithin C alleviates pancreatic β-cell dysfunction in type 1 diabetes by activating Nrf2 signaling.

Nutrition & diabetes

Luo C,Hou C,Yang D,Tan T,Chao C

PA5-27882 was used in Immunocytochemistry to explore the mechanism underlying urolithin C (UC)-mediated regulation of β-cell function.

Fri Dec 01 00:00:00 UTC 2023

Nrf2 as a therapeutic target in acetaminophen hepatotoxicity: A case study with sulforaphane.

Journal of biochemical and molecular toxicology

Etemadi Y,Akakpo JY,Ramachandran A,Jaeschke H

PA5-27882 was used in Western Blot to assess if therapeutic activation of Nrf2 is a viable approach to treat liver injury after APAP overdose.

Fri Dec 01 00:00:00 UTC 2023

Neuroprotective mechanism of human umbilical cord mesenchymal stem cell-derived extracellular vesicles improving the phenotype polarization of microglia via the PI3K/AKT/Nrf2 pathway in vascular dementia.

Synapse (New York, N.Y.)

Wang P,Yi T,Mao S,Li M

PA5-27882 was used in Western Blot to explore the mechanism of hUCMSC-Evs in VaD. VaD rat model was established by bilateral common carotid artery ligation and hUCMSC-Evs were extracted.

Sat Jul 01 00:00:00 UTC 2023

Aberrant somatic calcium channel function in cNurr1 and LRRK2-G2019S mice.

NPJ Parkinson's disease

Skiteva O,Yao N,Mantas I,Zhang X,Perlmann T,Svenningsson P,Chergui K

PA5-27882 was used in Western Blotting to investigate T-type and L-type VGCCs in SNc-DA neurons of two mouse models of Parkinson's disease.

Fri Apr 07 00:00:00 UTC 2023

Preventive Effect of Limosilactobacillus fermentum SCHY34 on Lead Acetate-Induced Neurological Damage in SD Rats.

Frontiers in nutrition

Long X,Wu H,Zhou Y,Wan Y,Kan X,Gong J,Zhao X

PA5-27882 was used in Western Blotting to show that L. fermentum SCHY34 showed a great biological scavenging and potential effect on alleviating the toxicity of lead ions.

Sat Jul 16 00:00:00 UTC 2022

Astragaloside IV protects against ischemia/reperfusion (I/R)-induced kidney injury based on the Keap1-Nrf2/ARE signaling pathway.

Translational andrology and urology

Su Y,Xu J,Chen S,Feng J,Li J,Lei Z,Qiao L,Wang Y,Zeng D

Published figure using Nrf2 polyclonal antibody (Product # PA5-27882) in Immunohistochemistry

Mon Aug 01 00:00:00 UTC 2022

Astragaloside IV protects against ischemia/reperfusion (I/R)-induced kidney injury based on the Keap1-Nrf2/ARE signaling pathway.

Translational andrology and urology

Su Y,Xu J,Chen S,Feng J,Li J,Lei Z,Qiao L,Wang Y,Zeng D

Published figure using Nrf2 polyclonal antibody (Product # PA5-27882) in Immunohistochemistry

Mon Aug 01 00:00:00 UTC 2022

E3 ligase TRIM15 facilitates non-small cell lung cancer progression through mediating Keap1-Nrf2 signaling pathway.

Cell communication and signaling : CCS

Liang M,Wang L,Sun Z,Chen X,Wang H,Qin L,Zhao W,Geng B

Published figure using Nrf2 polyclonal antibody (Product # PA5-27882) in Immunohistochemistry

Mon May 09 00:00:00 UTC 2022

E3 ligase TRIM15 facilitates non-small cell lung cancer progression through mediating Keap1-Nrf2 signaling pathway.

Cell communication and signaling : CCS

Liang M,Wang L,Sun Z,Chen X,Wang H,Qin L,Zhao W,Geng B

Published figure using Nrf2 polyclonal antibody (Product # PA5-27882) in Immunohistochemistry

Mon May 09 00:00:00 UTC 2022

Nrf2 expands the intracellular pool of the chaperone AHSP in a cellular model of β-thalassemia.

Redox biology

Han G,Cao C,Yang X,Zhao GW,Hu XJ,Yu DL,Yang RF,Yang K,Zhang YY,Wang WT,Liu XZ,Xu P,Liu XH,Chen P,Xue Z,Liu DP,Lv X

PA5-27882 was used in ChIP Assay, Immunocytochemistry, Immunohistochemistry (Paraffin), Immunoprecipitation, Western Blot, Flow Cytometry to demonstrate Nrf2-mediated feedback regulation of AHSP in response to excess a-globin, as occurs in ß-thalassemia.

Fri Apr 01 00:00:00 UTC 2022

Evogliptin Directly Inhibits Inflammatory and Fibrotic Signaling in Isolated Liver Cells.

International journal of molecular sciences

Seo HY,Lee SH,Han E,Hwang JS,Han S,Kim MK,Jang BK

PA5-27882 was used in Western Blotting to investigate whether evogliptin directly inhibits inflammatory and fibrotic signaling.

Sat Oct 01 00:00:00 UTC 2022

A steroid receptor coactivator small molecule "stimulator" attenuates post-stroke ischemic brain injury.

Frontiers in molecular neuroscience

McClendon LK,Garcia RL,Lazaro T,Robledo A,Vasandani V,Luna ZAE,Rao AS,Srivatsan A,Lonard DM,Dacso CC,Kan P,O'Malley BW

PA5-27882 was used in Immunohistochemistry to investigate if an MCB-613 derivative (MCB-10-1), could protect the brain following ischemic injury.

Thu Dec 22 00:00:00 UTC 2022

Regulation of VEGFA, KRAS, and NFE2L2 Oncogenes by MicroRNAs in Head and Neck Cancer.

International journal of molecular sciences

Cuzziol CI,Marzochi LL,Possebon VS,Kawasaki-Oyama RS,Mattos MF,Junior VS,Ferreira LAM,Pavarino ÉC,Castanhole-Nunes MMU,Goloni-Bertollo EM

PA5-27882 was used in Western Blotting to investigate if miRNAs hsa-miR-17-5p, hsa-miR-140-5p, and hsa-miR-874-3p could interfere in VEGFA, KRAS, and NFE2L2 expression in cell lines derived from head and neck cancer (HNC).

Tue Jul 05 00:00:00 UTC 2022

Ficus erecta Thunb Leaves Alleviate Memory Loss Induced by Scopolamine in Mice via Regulation of Oxidative Stress and Cholinergic System.

Molecular neurobiology

Sohn E,Kim YJ,Kim JH,Jeong SJ

PA5-27882 was used in Western Blotting to examine the neuropharmacological effects of ethanol extract of Ficus erecta Thunb leaves on cognitive dysfunction in a scopolamine-induced memory impairment animal model.

Sun Aug 01 00:00:00 UTC 2021

Ficus erecta Thunb Leaves Alleviate Memory Loss Induced by Scopolamine in Mice via Regulation of Oxidative Stress and Cholinergic System.

Molecular neurobiology

Sohn E,Kim YJ,Kim JH,Jeong SJ

Sun Aug 01 00:00:00 UTC 2021

The Role of Nrf2 Transcription Factor and Sp1-Nrf2 Protein Complex in Glutamine Transporter SN1 Regulation in Mouse Cortical Astrocytes Exposed to Ammonia.

International journal of molecular sciences

Dąbrowska K,Skowrońska K,Popek M,Albrecht J,Zielińska M

PA5-27882 was used in Western Blotting and ChIP Assay to test the hypothesis that activation of Nrf2 is the process that links ammonia-induced OS formation in astrocytes to downregulation and inactivation of SN1 and that it may involve the formation of a complex between Nrf2 and Sp1.

Mon Oct 18 00:00:00 UTC 2021

The Role of Nrf2 Transcription Factor and Sp1-Nrf2 Protein Complex in Glutamine Transporter SN1 Regulation in Mouse Cortical Astrocytes Exposed to Ammonia.

International journal of molecular sciences

Dąbrowska K,Skowrońska K,Popek M,Albrecht J,Zielińska M

Mon Oct 18 00:00:00 UTC 2021

The Role of Nrf2 Transcription Factor and Sp1-Nrf2 Protein Complex in Glutamine Transporter SN1 Regulation in Mouse Cortical Astrocytes Exposed to Ammonia.

International journal of molecular sciences

Dąbrowska K,Skowrońska K,Popek M,Albrecht J,Zielińska M

Mon Oct 18 00:00:00 UTC 2021

P- Hydroxybenzyl Alcohol Alleviates Oxidative Stress in a Nonalcoholic Fatty Liver Disease Larval Zebrafish Model and a BRL-3A Hepatocyte Via the Nrf2 Pathway.

Frontiers in pharmacology

An J,Cheng L,Yang L,Song N,Zhang J,Ma K,Ma J

Published figure using Nrf2 polyclonal antibody (Product # PA5-27882) in Immunocytochemistry

Fri Apr 30 00:00:00 UTC 2021

Biochemical study on the protective effect of curcumin on acetaminophen and gamma-irradiation induced hepatic toxicity in rats.

Environmental toxicology

Eassawy MMT,Salem AA,Ismail AFM

Published figure using Nrf2 polyclonal antibody (Product # PA5-27882) in Western Blot

Thu Dec 17 00:00:00 UTC 2020

Sulforaphane Protects Piglet Brains from Neonatal Hypoxic-Ischemic Injury.

Developmental neuroscience

Wang B,Kulikowicz E,Lee JK,Koehler RC,Yang ZJ

PA5-27882 was used in Western Blot to conclude that systemic administration of sulforaphane 15 min after HI can induce the translocation of Nrf2 to the nucleus, increase expression of an enzyme involved in glutathione synthesis, and salvage neurons in the highly vulnerable putamen and sensorimotor cortex in a large-animal model of HI.

Tue Oct 26 00:00:00 UTC 2021

Sulforaphane Protects Piglet Brains from Neonatal Hypoxic-Ischemic Injury.

Developmental neuroscience

Wang B,Kulikowicz E,Lee JK,Koehler RC,Yang ZJ

Tue Oct 26 00:00:00 UTC 2021

Polarization of Low-Grade Inflammatory Monocytes Through TRAM-Mediated Up-Regulation of Keap1 by Super-Low Dose Endotoxin.

Frontiers in immunology

Rahtes A,Li L

PA5-27882 was used in Immunoprecipitation to show bacterial endotoxin exposure at low levels can result in murine monocyte accumulation of Nrf2-inhibitory protein Keap1.

Mon Apr 19 00:00:00 UTC 2021

Polarization of Low-Grade Inflammatory Monocytes Through TRAM-Mediated Up-Regulation of Keap1 by Super-Low Dose Endotoxin.

Frontiers in immunology

Rahtes A,Li L

PA5-27882 was used in Immunoprecipitation to show bacterial endotoxin exposure at low levels can result in murine monocyte accumulation of Nrf2-inhibitory protein Keap1.

Mon Apr 19 00:00:00 UTC 2021

Polarization of Low-Grade Inflammatory Monocytes Through TRAM-Mediated Up-Regulation of Keap1 by Super-Low Dose Endotoxin.

Frontiers in immunology

Rahtes A,Li L

PA5-27882 was used in Immunoprecipitation to show bacterial endotoxin exposure at low levels can result in murine monocyte accumulation of Nrf2-inhibitory protein Keap1.

Mon Apr 19 00:00:00 UTC 2021

Oxidative Stress Modulation by Cameroonian Spice Extracts in HepG2 Cells: Involvement of Nrf2 and Improvement of Glucose Uptake.

Metabolites

Nwakiban APA,Cicolari S,Piazza S,Gelmini F,Sangiovanni E,Martinelli G,Bossi L,Carpentier-Maguire E,Tchamgoue AD,Agbor G,Kuiaté JR,Beretta G,Dell'Agli M,Magni P

PA5-27882 was used in Immunocytochemistry-immunofluorescence to evaluate the antioxidant capacity and the effects on oxidative-stress-induced impairment of glucose uptake of 11 Cameroonian spice extracts.

Fri May 01 00:00:00 UTC 2020

Oxidative Stress Modulation by Cameroonian Spice Extracts in HepG2 Cells: Involvement of Nrf2 and Improvement of Glucose Uptake.

Metabolites

Nwakiban APA,Cicolari S,Piazza S,Gelmini F,Sangiovanni E,Martinelli G,Bossi L,Carpentier-Maguire E,Tchamgoue AD,Agbor G,Kuiaté JR,Beretta G,Dell'Agli M,Magni P

Fri May 01 00:00:00 UTC 2020

VEGFA and NFE2L2 Gene Expression and Regulation by MicroRNAs in Thyroid Papillary Cancer and Colloid Goiter.

Genes

Stuchi LP,Castanhole-Nunes MMU,Maniezzo-Stuchi N,Biselli-Chicote PM,Henrique T,Padovani Neto JA,de-Santi Neto D,Girol AP,Pavarino EC,Goloni-Bertollo EM

PA5-27882 was used in Immunohistochemistry to suggest that hsa-miR-17-5p may be a positive regulator of NFE2L2 expression in PTC.

Wed Aug 19 00:00:00 UTC 2020

Chloroquine reverses chemoresistance via upregulation of p21^WAF1/CIP1 and autophagy inhibition in ovarian cancer.

Cell death & disease

Hwang JR,Kim WY,Cho YJ,Ryu JY,Choi JJ,Jeong SY,Kim MS,Kim JH,Paik ES,Lee YY,Han HD,Lee JW

PA5-27882 was used in Western Blotting to demonstrate that CQ increases cytotoxicity in combination with CDDP by inducing lethal DNA damage by induction of p21WAF1/CIP1 expression and autophagy inhibition in CDDP-resistant EOC.

Fri Dec 04 00:00:00 UTC 2020

Human umbilical artery endothelial cells from Large-for-Gestational-Age newborn have increased antioxidant efficiency and gene expression.

Journal of cellular physiology

Carrasco-Wong I,Hernández C,Jara-Gutiérrez C,Porras O,Casanello P

PA5-27882 was used in ChIP Assay, Immunocytochemistry, Immunohistochemistry (Paraffin), Immunoprecipitation, Western Blot to propose that a chronic pro-oxidant intrauterine milieu, as evidenced in pregestational obesity, could induce a more efficient antioxidant system in fetal vascular cells, which could be maintained by epigenetic mechanism during postnatal life.

Thu Aug 01 00:00:00 UTC 2019

NRF2 Activation Inhibits Both TGF-β1- and IL-13-Mediated Periostin Expression in Fibroblasts: Benefit of Cinnamaldehyde for Antifibrotic Treatment.

Oxidative medicine and cellular longevity

Mitamura Y,Murai M,Mitoma C,Furue M

PA5-27882 was used in Western Blotting to study the role of oxidative stress in the expression of POSTN induced by TGF-Β1 and IL-13 in dermal fibroblasts.

Wed Jan 02 00:00:00 UTC 2019

Myokine irisin-induced protection against oxidative stress in vitro. Involvement of heme oxygenase-1 and antioxidazing enzymes superoxide dismutase-2 and glutathione peroxidase.

Journal of physiology and pharmacology : an official journal of the Polish Physiological Society

Mazur-Bialy AI,Kozlowska K,Pochec E,Bilski J,Brzozowski T

PA5-27882 was used in Western Blotting to determine the ability of irisin to interact with reactive oxygen species in RAW 264.7 murine macrophages.

Thu Feb 01 00:00:00 UTC 2018

Acute maternal oxidant exposure causes susceptibility of the fetal brain to inflammation and oxidative stress.

Journal of neuroinflammation

Akhtar F,Rouse CA,Catano G,Montalvo M,Ullevig SL,Asmis R,Kharbanda K,Maffi SK

Published figure using Nrf2 polyclonal antibody (Product # PA5-27882) in Western Blot

Sat Sep 30 00:00:00 UTC 2017

Sodium fluoride affects zebrafish behaviour and alters mRNA expressions of biomarker genes in the brain: Role of Nrf2/Keap1.

Environmental toxicology and pharmacology

Mukhopadhyay D,Priya P,Chattopadhyay A

PA5-27882 was used in western blot to elucidate the molecular mechanism of fluoride-induced neurotoxicity using zebrafish

Tue Sep 01 00:00:00 UTC 2015

Acetyl-l-carnitine prevents homocysteine-induced suppression of Nrf2/Keap1 mediated antioxidation in human lens epithelial cells.

Molecular medicine reports

Yang SP,Yang XZ,Cao GP

PA5-27882 was used in western blot to prevent homocysteine-induced demethylation by administering acetyl-l-carnitine to human lens epithelial cells.

Wed Jul 01 00:00:00 UTC 2015

Bioinformatics

Protein Aliases: E4TF1-60; E4TF1A; NF-E2-related factor 2; NFE2-related factor 2; NFT2; nrf2; NRF2A; nuclear factor erythroid 2-like 2; Nuclear factor erythroid 2-related factor 2; nuclear factor erythroid-derived 2-like 2; nuclear factor, erythroid 2-like 2; Nuclear factor, erythroid derived 2, like 2

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Gene Aliases: AI194320; HEBP1; NFE2L2; NRF2

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UniProt ID: (Human) Q16236, (Mouse) Q60795, (Rat) O54968

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Entrez Gene ID: (Human) 4780, (Mouse) 18024, (Rat) 83619

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Function(s)

transcription regulatory region sequence-specific DNA binding RNA polymerase II distal enhancer sequence-specific DNA binding RNA polymerase II activating transcription factor binding transcriptional activator activity, RNA polymerase II distal enhancer sequence-specific binding transcription cofactor binding DNA binding transcription factor activity, sequence-specific DNA binding protein binding protein domain specific binding transcription regulatory region DNA binding sequence-specific DNA binding protein heterodimerization activity

Process(es)

Performance Guarantee

If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

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Nrf2 Polyclonal Antibody

Nrf2 Antibody (PA5-27882) in ICC/IF

Product Details

PA5-27882

Western Blot (WB)

Immunohistochemistry (IHC)

Immunohistochemistry (Paraffin) (IHC (P))

Immunocytochemistry (ICC/IF)

Flow Cytometry (Flow)

Immunoprecipitation (IP)

ChIP assay (ChIP)

Miscellaneous PubMed (MISC)

Species Reactivity

Published species

Host/Isotype

Class

Type

Immunogen

Conjugate

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

Shipping conditions

RRID

Product Specific Information

Target Information

Bioinformatics

Performance Guarantee

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