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Invitrogen
NuMA Polyclonal Antibody
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NuMA Antibody (PA3-16829) in ICC/IF
Immunofluorescence analysis of NuMA was performed using HeLa cells. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 15 minutes, and blocked with 2% BSA for 1 hour at room temperature. The cells were labeled with NuMA Polyclonal Antibody (Product # PA3-16829) at 1:200 dilution in 0.1% BSA, incubated at 4 degree celsius overnight and then labeled with Goat anti-Rabbit IgG (Heavy Chain) Superclonal™ Recombinant Secondary Antibody, Alexa Fluor® 488 conjugate (Product # A27034) at a diluti... View More
Product Details
PA3-16829
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Marsupial,
Mouse,
Non-human primate,
Rat
Published species
Human
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
Full-length recombinant human NuMA expressed in insect Sf9 cells using baculovirus expression system
Conjugate
Form
Liquid
Concentration
Conc. Not Determined
Storage buffer
whole serum
Contains
0.02% sodium azide
Storage conditions
-20°C or -80°C if preferred
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
AB_2267417
Product Specific Information
Suggested positive control: Hela whole cell extract.
Target Information
NuMA (nuclear mitotic apparatus) is a long coil-coiled protein that plays a role in the interphase and mitosis phases of a cell cycle. In interphase, it has been hypothesized to play a structural role in the nucleoskeleton that may be important in nuclear organization and functions. During mitosis, it is associated with spindle microtubule organization and chromosome positioning.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Bioinformatics
Protein Aliases: centrophilin stabilizes mitotic spindle in mitotic cells; NMP-22; Nuclear matrix protein-22; Nuclear mitotic apparatus protein; Nuclear mitotic apparatus protein 1; NuMA protein; SP-H antigen; structural nuclear protein
Gene Aliases: 6720401E04Rik; AA764025; AL022610; AU014979; NMP-22; NMP22; NUMA; NUMA1
UniProt ID: (Human) Q14980, (Mouse) E9Q7G0
Entrez Gene ID: (Human) 4926, (Rat) 308870, (Mouse) 101706
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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