Invitrogen

Nuclear Matrix Protein p84 Polyclonal Antibody

Nuclear Matrix Protein p84 Antibody in Immunocytochemistry (ICC/IF)

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Nuclear Matrix Protein p84 Antibody (PA5-27816) in ICC/IF

Nuclear Matrix Protein p84 Polyclonal Antibody detects Nuclear Matrix Protein p84 protein at cytoplasm and nucleus by immunofluorescent analysis. Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: Nuclear Matrix Protein p84 protein stained by Nuclear Matrix Protein p84 Polyclonal Antibody (Product # PA5-27816) diluted at 1:500. Blue: Hoechst 33342 staining.

Nuclear Matrix Protein p84 Antibody in Immunohistochemistry (Paraffin) (IHC (P))

Nuclear Matrix Protein p84 Antibody in Western Blot (WB)

Nuclear Matrix Protein p84 Antibody in Immunoprecipitation (IP)

Nuclear Matrix Protein p84 Antibody in ChIP Assay (ChIP)

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Product Details

PA5-27816

Applications

Tested Dilution

Publications

Western Blot (WB)

1:500-1:3,000

View 6 publications 6 publications

Immunohistochemistry (Paraffin) (IHC (P))

1:100-1:1,000

Immunocytochemistry (ICC/IF)

1:100-1:1,000

Immunoprecipitation (IP)

1:100-1:500

ChIP assay (ChIP)

Assay-dependent

Product Specifications

Species Reactivity

Avian, Human, Mouse, Rat

Published species

Human

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

Recombinant protein encompassing a sequence within the C-terminus region of human Nuclear Matrix Protein p84. The exact sequence is proprietary.

View immunogen

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

1 mg/mL

Purification

Antigen affinity chromatography

Storage buffer

PBS, pH 7, with 20% glycerol, 1% BSA

Contains

0.01% thimerosal

Storage conditions

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

Shipping conditions

Ambient (domestic); Wet ice (international)

AB_2545292

Product Specific Information

Recommended positive controls: 293T, A431, HeLa, HepG2, PC-12, Rat-2, THOC1 shRNA-transfected 293T.

Predicted reactivity: Mouse (96%), Rat (96%), Chicken (89%), Rhesus Monkey (100%), Bovine (98%).

Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.

Target Information

HPR1 is part of the TREX (transcription/export) complex, which includes TEX1 (MIM 606929), THO2 (MIM 300395), ALY (MIM 604171), and UAP56 (MIM 142560).

⚠WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause birth defects or other reproductive harm. For more information go to www.P65Warnings.ca.gov.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

ITGBL1 promotes EMT, invasion and migration by activating NF-κB signaling pathway in prostate cancer.

OncoTargets and therapy

Li W,Li S,Yang J,Cui C,Yu M,Zhang Y

PA5-27816 was used in Western Blotting to study the role of ITGBL1 in prostate cancer.

Thu Oct 01 00:00:00 UTC 2020

Downregulation of miR‑505‑3p predicts poor bone metastasis‑free survival in prostate cancer.

Oncology reports

Tang Y,Wu B,Huang S,Peng X,Li X,Huang X,Zhou W,Xie P,He P

PA5-27816 was used in Western Blotting to that miR-505-3p may serve as a predictive marker for bone metastasis of prostate cancer.

Tue Jan 01 00:00:00 UTC 2019

N-Myc promotes therapeutic resistance development of neuroendocrine prostate cancer by differentially regulating miR-421/ATM pathway.

Molecular cancer

Yin Y,Xu L,Chang Y,Zeng T,Chen X,Wang A,Groth J,Foo WC,Liang C,Hu H,Huang J

PA5-27816 was used in Western Blotting to determine the molecular mechanisms underlying the N-Myc- driven therapeutic resistance.

Fri Jan 18 00:00:00 UTC 2019

Downregulation of miR‑19a‑3p promotes invasion, migration and bone metastasis via activating TGF‑β signaling in prostate cancer.

Oncology reports

Wa Q,Li L,Lin H,Peng X,Ren D,Huang Y,He P,Huang S

Published figure using Nuclear Matrix Protein p84 polyclonal antibody (Product # PA5-27816) in Western Blot

Mon Jan 01 00:00:00 UTC 2018

Oncogenic miR-210-3p promotes prostate cancer cell EMT and bone metastasis via NF-κB signaling pathway.

Molecular cancer

Ren D,Yang Q,Dai Y,Guo W,Du H,Song L,Peng X

PA5-27816 was used in Western Blotting to demonstrate that miR-210-3p maintains the sustained activation of NF-κB signaling via targeting negative regulators of NF-κB signaling (TNF-α Induced Protein 3 Interacting Protein 1) TNIP1 and (Suppressor Of Cytokine Signaling 1) SOCS1, resulting in EMT, invasion, migration and bone metastasis of PCa cells.

Mon Jul 10 00:00:00 UTC 2017

Downregulation of miR-141-3p promotes bone metastasis via activating NF-κB signaling in prostate cancer.

Journal of experimental & clinical cancer research : CR

Huang S,Wa Q,Pan J,Peng X,Ren D,Huang Y,Chen X,Tang Y

PA5-27816 was used in Western Blotting to unravel a novel mechanism underlying the bone metastasis of PCa, suggesting that miR-141-3p mimics might represent a potential therapeutic avenue for the treatment of PCa bone metastasis.

Mon Dec 04 00:00:00 UTC 2017

Bioinformatics

Protein Aliases: hTREX84; Nuclear matrix protein p84; p84N5; THO complex subunit 1; tho1

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Gene Aliases: 3110002N20Rik; AW107452; HPR1; LRRGT00070; NMP-84; P84; P84N5; THOC1

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UniProt ID: (Human) Q96FV9, (Mouse) Q8R3N6

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Entrez Gene ID: (Human) 9984, (Mouse) 225160, (Rat) 291797

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Function(s)

DNA binding RNA binding protein binding

Process(es)

regulation of DNA recombination termination of RNA polymerase II transcription RNA processing RNA export from nucleus mRNA export from nucleus apoptotic process signal transduction RNA splicing mRNA 3'-end processing replication fork processing regulation of DNA-templated transcription, elongation positive regulation of DNA-templated transcription, elongation viral mRNA export from host cell nucleus negative regulation of isotype switching to IgA isotypes negative regulation of DNA damage checkpoint transcription, DNA-templated regulation of transcription, DNA-templated mRNA processing transport regulation of apoptotic process mRNA transport

Performance Guarantee

If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

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Nuclear Matrix Protein p84 Polyclonal Antibody

Nuclear Matrix Protein p84 Antibody (PA5-27816) in ICC/IF

Product Details

PA5-27816

Western Blot (WB)

Immunohistochemistry (Paraffin) (IHC (P))

Immunocytochemistry (ICC/IF)

Immunoprecipitation (IP)

ChIP assay (ChIP)

Species Reactivity

Published species

Host/Isotype

Class

Type

Immunogen

Conjugate

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

Shipping conditions

RRID

Product Specific Information

Target Information

Bioinformatics

Performance Guarantee

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