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Invitrogen
Nucleostemin Polyclonal Antibody
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Nucleostemin Antibody (PA5-143898) in ICC/IF
Immunocytochemistry analysis of Nucleostemin in HEP3B cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent for 15 mins. The cells were blocked with 10% goat serum. Samples were then incubated in Nucleostemin Polyclonal antibody (Product # PA5-143898) using a dilution of 5 μg/mL. DyLight®488 Conjugated Goat Anti-Rabbit IgG was used as secondary antibody at 1:100 dilution a... View More
Product Details
PA5-143898
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse,
Rat
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
E.coli-derived human Nucleostemin/GNL3 recombinant protein (Position: R45-V549).
Conjugate
Form
Lyophilized
Concentration
500 µg/mL
Purification
Antigen affinity chromatography
Storage buffer
PBS with 4mg trehalose
Contains
no preservative
Storage conditions
-20°C
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
AB_3075112
Product Specific Information
Adding 0.2 mL of distilled water will yield a concentration of 500 µg/mL.
Positive Control - WB: human Hela whole cell, human K562 whole cell, human PC-3 whole cell, human Jurkat whole cell, rat C6 whole cell, mouse NIH/3T3 whole cell. IHC: human appendiceal adenocarcinoma tissue, human breast cancer tissue, human colonic adenocarcinoma tissue, human gall bladder adenosquamous carcinoma tissue. ICC/IF: HEP3B cell. Flow: Hela cell.|Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.
Target Information
Arylsulfatase F, also known as ARSF, is a 590 amino acid secretory protein that belongs to the sulfatase family of bone and cartilage matrix proteins. Arylsulfatase F uses calcium as a cofactor to catalyze reactions that are important in maintaining correct bone composition. The activity of Arylsulfatase F, unlike that of other family members, such as Arylsulfatase E, is not inhibited by warfarin. The gene encoding Arylsulfatase F maps to human chromosome X, which contains nearly 153 million base pairs and houses over 1,000 genes. In conjunction with chromosome Y, chromosome X is responsible for sex determination. There are a number of conditions related to an abnormal number and combination of sex chromosomes, some of which include Turner's syndrome, color blindness, hemophilia and Duchenne muscular dystrophy.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: E2-induced gene 3 protein; estradiol-induced nucleotide binding protein; guanine nucleotide binding protein-like 3 (nucleolar); Guanine nucleotide-binding protein-like 3; MGC800; NNP47; Novel nucleolar protein 47; Nucleolar GTP-binding protein 3; Nucleostemin
Gene Aliases: C77032; E2IG3; GNL3; NNP47; NS
UniProt ID: (Human) Q9BVP2, (Mouse) Q8CI11, (Rat) Q811S9
Entrez Gene ID: (Human) 26354, (Mouse) 30877, (Rat) 290556
cell proliferation
regulation of cell proliferation
ribosome biogenesis
positive regulation of pri-miRNA transcription from RNA polymerase II promoter
metabolic process
positive regulation of telomere maintenance
positive regulation of protein sumoylation
positive regulation of protein localization to chromosome, telomeric region
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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