Proteintech

Nurr1/NR4A2 Polyclonal Antibody

Nurr1/NR4A2 Antibody in Immunocytochemistry (ICC/IF)

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Nurr1/NR4A2 Antibody (10975-2-AP) in ICC/IF

Immunofluorescent analysis of ( 4% PFA) fixed HepG2 cells using 10975-2-AP (Nurr1/NR4A2 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IGG (H+L).

Nurr1/NR4A2 Antibody in Immunohistochemistry (Paraffin) (IHC (P))

Nurr1/NR4A2 Antibody in Western Blot (WB)

Product Image Gallery

Product Details

10975-2-AP

Applications

Tested Dilution

Publications

Western Blot (WB)

1:500-1:1,000

View 11 publications 11 publications

Immunohistochemistry (IHC)

View 3 publications 3 publications

Immunohistochemistry (Paraffin) (IHC (P))

1:50-1:500

Immunocytochemistry (ICC/IF)

1:50-1:500

View 5 publications 5 publications

ChIP assay (ChIP)

View 1 publication 1 publication

Product Specifications

Species Reactivity

Human

Published species

Human, Rat

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

Nurr1/NR4A2 Fusion Protein Ag1418 (250-598 aa encoded by BC009288)

View immunogen

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

0.24 mg/mL

Purification

Antigen affinity chromatography

Storage buffer

PBS, pH 7.3, with 50% glycerol

Contains

0.02% sodium azide

Storage conditions

-20°C

Shipping conditions

Wet ice (domestic); Dry ice (international)

Product Specific Information

Immunogen sequence: SPPSRGSPS NEGLCAVCGD NAACQHYGVR TCEGCKGFFK RTVQKNAKYV CLANKNCPVD KRRRNRCQYC RFQKCLAVGM VKEVVRTDSL KGRRGRLPSK PKSPQEPSPP SPPVSLISAL VRAHVDSNPA MTSLDYSRFQ ANPDYQMSGD DTQHIQQFYD LLTGSMEIIR GWAEKIPGFA DLPKADQDLL FESAFLELFV LRLAYRSNPV EGKLIFCNGV VLHRLQCVRG FGEWIDSIVE FSSNLQNMNI DISAFSCIAA LAMVTERHGL KEPKRVEELQ NKIVNCLKDH VTFNNGGLNR PNYLSKLLGK LPELRTLCTQ GLQRIFYLKL EDLVPPPAII DKLFLDTLPF (250-598 aa encoded by BC009288)

Target Information

The Nuclear receptor related 1 protein (Nurr1) also known as NR4A2 (nuclear receptor subfamily 4, group A, member 2) is a protein that in humans is encoded by the NR4A2 gene. NURR1 is a member of the nuclear receptor family of intracellular transcription factors.Nurr1 plays a key role in the maintenance of the dopaminergic system of the brain. Mutations in this gene have been associated with disorders related to dopaminergic dysfunction, including Parkinson's disease, schizophrenia, and manic depression. Misregulation of this gene may be associated with rheumatoid arthritis. Four transcript variants encoding four distinct isoforms have been identified for this gene. Additional alternate splice variants may exist, but their full-length nature has not been determined.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

Protective effect of Nr4a2 (Nurr1) against LPS-induced depressive-like behaviors via regulating activity of microglia and CamkII neurons in anterior cingulate cortex.

Pharmacological research

He Y,Wang Y,Yu H,Tian Y,Chen X,Chen C,Ren Y,Chen Z,Ren Y,Gong X,Cheng K,Liu X,Zhong L,Guo Y,Xie P

Mon May 01 00:00:00 UTC 2023

Single-nuclei RNA sequencing uncovers heterogenous transcriptional signatures in Parkinson's disease associated with nuclear receptor-related factor 1 defect.

Neural regeneration research

Kambey PA,Liu WY,Wu J,Bosco B,Nadeem I,Kanwore K,Gao DS

Fri Sep 01 00:00:00 UTC 2023

Single-nuclei RNA sequencing uncovers heterogenous transcriptional signatures in Parkinson's disease associated with nuclear receptor-related factor 1 defect.

Neural regeneration research

Kambey PA,Liu WY,Wu J,Bosco B,Nadeem I,Kanwore K,Gao DS

Fri Sep 01 00:00:00 UTC 2023

Targeting Microglial Metabolic Rewiring Synergizes with Immune-Checkpoint Blockade Therapy for Glioblastoma.

Cancer discovery

Ye Z,Ai X,Yang K,Yang Z,Fei F,Liao X,Qiu Z,Gimple RC,Yuan H,Huang H,Gong Y,Xiao C,Yue J,Huang L,Saulnier O,Wang W,Zhang P,Dai L,Wang X,Wang X,Ahn YH,You C,Xu J,Wan X,Taylor MD,Zhao L,Rich JN,Zhou S

Mon Apr 03 00:00:00 UTC 2023

FASEB bioAdvances

Sun X,Kato H,Sato H,Han X,Hirofuji Y,Kato TA,Sakai Y,Ohga S,Fukumoto S,Masuda K

Fri Jul 01 00:00:00 UTC 2022

LincRNA-p21 Upregulates Nuclear Orphan Receptor Nr4a2 and Aggravates Myocardial Ischemia/Reperfusion Injury via Targeting MiR-466i-5p.

International heart journal

Zhai X,Liu R,Li J,Wang F,Liu L,Wei S,Bian Y,Pang J,Xue M,Qin D,Wang S,Xu T,Xu F,Cao S,Yuan Q,Chen Y

Tue Oct 04 00:00:00 UTC 2022

Transfer of neuron-derived α-synuclein to astrocytes induces neuroinflammation and blood-brain barrier damage after methamphetamine exposure: Involving the regulation of nuclear receptor-associated protein 1.

Brain, behavior, and immunity

Huang J,Ding J,Wang X,Gu C,He Y,Li Y,Fan H,Xie Q,Qi X,Wang Z,Qiu P

Tue Nov 01 00:00:00 UTC 2022

Brain, behavior, and immunity

Huang J,Ding J,Wang X,Gu C,He Y,Li Y,Fan H,Xie Q,Qi X,Wang Z,Qiu P

Tue Nov 01 00:00:00 UTC 2022

Itaconate Attenuates Neuroinflammation and Exerts Dopamine Neuroprotection in Parkinson's Disease through Inhibiting NLRP3 Inflammasome.

Brain sciences

Sun G,Zhang R,Liu C,Meng W,Pang Q

Fri Sep 16 00:00:00 UTC 2022

Attenuated macrophage activation mediated by microRNA-183 knockdown through targeting NR4A2.

Experimental and therapeutic medicine

Gong FH,Long L,Yang YS,Shen DH,Zhang YS,Wang XS,Zhang XP,Xiao XQ

Thu Apr 01 00:00:00 UTC 2021

Hair-Follicle-Associated Pluripotent (HAP) Stem Cells Can Extensively Differentiate to Tyrosine-Hydroxylase-Expressing Dopamine-Secreting Neurons.

Cells

Yamane M,Takaoka N,Obara K,Shirai K,Aki R,Hamada Y,Arakawa N,Hoffman RM,Amoh Y

Sat Apr 10 00:00:00 UTC 2021

Pramipexole attenuates 6-OHDA-induced Parkinson's disease by mediating the Nurr1/NF-κB pathway.

Molecular biology reports

Gao H,Wang D,Wang YL,Mao JP,Jiang S,Yang XL

Thu Apr 01 00:00:00 UTC 2021

Caveolin-1 facilitates cell migration by upregulating nuclear receptor 4A2/retinoid X receptor α-mediated β-galactoside α2,6-sialyltransferase I expression in human hepatocarcinoma cells.

The international journal of biochemistry & cell biology

Chen X,Wang L,Yu X,Wang S,Zhang J

Sun Aug 01 00:00:00 UTC 2021

Caveolin-1 facilitates cell migration by upregulating nuclear receptor 4A2/retinoid X receptor α-mediated β-galactoside α2,6-sialyltransferase I expression in human hepatocarcinoma cells.

The international journal of biochemistry & cell biology

Chen X,Wang L,Yu X,Wang S,Zhang J

Sun Aug 01 00:00:00 UTC 2021

Wnt1 silencing enhances neurotoxicity induced by paraquat and maneb in SH-SY5Y cells.

Experimental and therapeutic medicine

Huang C,Ma J,Li BX,Sun Y

Fri Nov 01 00:00:00 UTC 2019

Correlation between Nurr1 expression and drug resistance in the brain of rats with epilepsy.

European review for medical and pharmacological sciences

Li HY,Liu F,Wang HR

Thu Mar 01 00:00:00 UTC 2018

Correlation between Nurr1 expression and drug resistance in the brain of rats with epilepsy.

European review for medical and pharmacological sciences

Li HY,Liu F,Wang HR

Thu Mar 01 00:00:00 UTC 2018

Correlation between Nurr1 expression and drug resistance in the brain of rats with epilepsy.

European review for medical and pharmacological sciences

Li HY,Liu F,Wang HR

Thu Mar 01 00:00:00 UTC 2018

Correlation between Nurr1 expression and drug resistance in the brain of rats with epilepsy.

European review for medical and pharmacological sciences

Li HY,Liu F,Wang HR

Thu Mar 01 00:00:00 UTC 2018

miR-206 modulates lipopolysaccharide-mediated inflammatory cytokine production in human astrocytes.

Cellular signalling

Duan X,Zohaib A,Li Y,Zhu B,Ye J,Wan S,Xu Q,Song Y,Chen H,Cao S

Thu Jan 01 00:00:00 UTC 2015

miR-206 modulates lipopolysaccharide-mediated inflammatory cytokine production in human astrocytes.

Cellular signalling

Duan X,Zohaib A,Li Y,Zhu B,Ye J,Wan S,Xu Q,Song Y,Chen H,Cao S

Thu Jan 01 00:00:00 UTC 2015

Identification of low intratumoral gene expression heterogeneity in neuroblastic tumors by genome-wide expression analysis and game theory.

Cancer

Albino D,Scaruffi P,Moretti S,Coco S,Truini M,Di Cristofano C,Cavazzana A,Stigliani S,Bonassi S,Tonini GP

Mon Sep 15 00:00:00 UTC 2008

Identification of low intratumoral gene expression heterogeneity in neuroblastic tumors by genome-wide expression analysis and game theory.

Cancer

Albino D,Scaruffi P,Moretti S,Coco S,Truini M,Di Cristofano C,Cavazzana A,Stigliani S,Bonassi S,Tonini GP

Mon Sep 15 00:00:00 UTC 2008

Identification of low intratumoral gene expression heterogeneity in neuroblastic tumors by genome-wide expression analysis and game theory.

Cancer

Albino D,Scaruffi P,Moretti S,Coco S,Truini M,Di Cristofano C,Cavazzana A,Stigliani S,Bonassi S,Tonini GP

Mon Sep 15 00:00:00 UTC 2008

Identification of low intratumoral gene expression heterogeneity in neuroblastic tumors by genome-wide expression analysis and game theory.

Cancer

Albino D,Scaruffi P,Moretti S,Coco S,Truini M,Di Cristofano C,Cavazzana A,Stigliani S,Bonassi S,Tonini GP

Mon Sep 15 00:00:00 UTC 2008

Bioinformatics

Protein Aliases: HZF3; Immediate-early response protein NOT; intermediate-early receptor protein; NGFI-B; NGFI-B beta; NGFI-B/nur77 beta-type transcription factor homolog; nuclear receptor related 1; Nuclear receptor subfamily 4 group A member 2; nuclear receptor subfamily 4, group A, member 2; nur related protein-1, human homolog of; orphan nuclear receptor NR4A2; Orphan nuclear receptor NURR1; T-cell nuclear receptor NOT; transcriptionally inducible nuclear receptor related 1; Transcriptionally-inducible nuclear receptor

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Gene Aliases: HZF-3; NOT; NR4A2; NURR1; RNR1; TINUR

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UniProt ID: (Human) P43354

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Entrez Gene ID: (Human) 4929

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Function(s)

RNA polymerase II core promoter sequence-specific DNA binding transcriptional activator activity, RNA polymerase II core promoter proximal region sequence-specific binding DNA binding steroid hormone receptor activity RNA polymerase II transcription factor activity, ligand-activated sequence-specific DNA binding protein binding beta-catenin binding zinc ion binding glucocorticoid receptor binding protein homodimerization activity retinoid X receptor binding protein heterodimerization activity

Process(es)

negative regulation of transcription from RNA polymerase II promoter response to hypoxia neuron migration response to amphetamine transcription, DNA-templated transcription initiation from RNA polymerase II promoter signal transduction adult locomotory behavior post-embryonic development central nervous system projection neuron axonogenesis habenula development intracellular receptor signaling pathway cellular response to extracellular stimulus cellular response to oxidative stress regulation of dopamine metabolic process dopamine biosynthetic process neuron maturation positive regulation of catalytic activity steroid hormone mediated signaling pathway negative regulation of neuron apoptotic process regulation of respiratory gaseous exchange fat cell differentiation positive regulation of transcription from RNA polymerase II promoter general adaptation syndrome canonical Wnt signaling pathway cellular response to corticotropin-releasing hormone stimulus dopaminergic neuron differentiation midbrain dopaminergic neuron differentiation negative regulation of apoptotic signaling pathway

Performance Guarantee

If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

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Nurr1/NR4A2 Polyclonal Antibody

Nurr1/NR4A2 Antibody (10975-2-AP) in ICC/IF

Product Details

10975-2-AP

Western Blot (WB)

Immunohistochemistry (IHC)

Immunohistochemistry (Paraffin) (IHC (P))

Immunocytochemistry (ICC/IF)

ChIP assay (ChIP)

Species Reactivity

Published species

Host/Isotype

Class

Type

Immunogen

Conjugate

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

Shipping conditions

Product Specific Information

Target Information

Bioinformatics

Performance Guarantee

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