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Invitrogen
O-linked N-acetylglucosamine (O-GlcNAc) Monoclonal Antibody (9D1.E4)
FIGURE: 1 / 3
O-linked N-acetylglucosamine (O-GlcNAc) Antibody (MA1-039) in WB
Western blot analysis of O-GlcNAc was performed by loading 10 µg of HEK293T or C2C12 whole cell lysates onto a 4-20% Tris-HCl polyacrylamide gel. Proteins were transferred to a PVDF membrane and blocked with 5% BSA/TBST for at least 1 hour. Membranes were then probed with a mouse monoclonal antibody recognizing O-GlcNAc (Product # MA1-039) at a dilution of 1:1000 overnight at 4°C on a rocking platform. Memb... View More
Product Details
MA1-039
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Chemical
Published species
Human
Host/Isotype
Mouse
/ IgG1
Class
Monoclonal
Type
Antibody
Clone
9D1.E4
Immunogen
SANM-conjugated linear peptide corresponding to human O-GluNAc.
Conjugate
Form
Liquid
Concentration
1 mg/mL
Purification
Protein A
Storage buffer
PBS with 1mg/mL BSA
Contains
0.05% sodium azide
Storage conditions
-20°C, Avoid Freeze/Thaw Cycles
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
AB_2536726
Product Specific Information
MA1-039 has been successfully used in Western blot and immunoprecipitation applications with human samples. In Western blot, MA1-039 detects proteins with O-GlcNAc post-translational modification, and therefore will detect multiple specific bands.
Target Information
O-linked N-acetylglucosamine (O-GlcNAc) is a posttranslational modification characterized by the attachment of N-acetylglucosamine to specific serine or threonine residues. Unlike other protein glycosylations, O-GlcNAc modifications occur within the nucleus and cytoplasm. They are found on many cellular proteins, including nuclear pore, oncogene, cytoskeletal, heat shock, viral and transcription regulatory proteins. O-GlcNAc glycosylations are thought to obscure phosphorylation sites, counteracting phosphorylation-dependent signaling pathways and protein interactions.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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