Proteintech

OCRL Polyclonal Antibody

OCRL Antibody in Immunohistochemistry (Paraffin) (IHC (P))

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OCRL Antibody (17695-1-AP) in IHC (P)

Immunohistochemistry of paraffin-embedded mouse kidney tissue slide using 17695-1-AP ( OCRL Antibody) at dilution of 1:200 (under 40x lens). heat mediated antigen retrieved with Tris-EDTA buffer (pH 9).

OCRL Antibody in Immunoprecipitation (IP)

Product Image Gallery

Product Details

17695-1-AP

Applications

Tested Dilution

Publications

Western Blot (WB)

1:500-1:2,000

View 4 publications 4 publications

Immunohistochemistry (IHC)

View 1 publication 1 publication

Immunohistochemistry (Paraffin) (IHC (P))

1:100-1:400

Immunocytochemistry (ICC/IF)

View 1 publication 1 publication

Immunoprecipitation (IP)

0.5-4.0 µg

Product Specifications

Species Reactivity

Human, Mouse, Rat

Published species

Human

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

OCRL Fusion Protein Ag11900 (538-893 aa encoded by BC094726)

View immunogen

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

0.13 mg/mL

Purification

Antigen affinity chromatography

Storage buffer

PBS, pH 7.3, with 50% glycerol

Contains

0.02% sodium azide

Storage conditions

-20°C

Shipping conditions

Wet ice (domestic); Dry ice (international)

Product Specific Information

This antibody is specific to OCRL.

Immunogen sequence: VDERRYRKV FEDSVRIMDR MENDFLPSLE LSRREFVFEN VKFRQLQKEK FQISNNGQVP CHFSFIPKLN DSQYCKPWLR AEPFEGYLEP NETVDISLDV YVSKDSVTIL NSGEDKIEDI LVLHLDRGKD YFLTISGNYL PSCFGTSLEA LCRMKRPIRE VPVTKLIDLE KSLLQMVPLD EGASERPLQV PKEIWLLVDH LFKYACHQED LFQTPGMQEE LQQIIDCLDT SIPETIPGSN HSVAEALLIF LEALPEPVIC YELYQRCLDS AYDPRICRQV ISQLPRCHRN VFRYLMAFLR ELLKFSEYNS VNANMIATLF TSLLLRPPPN LMARQTPSDR QRAIQFLLGF LLGSEED (538-893 aa encoded by BC094726 )

Target Information

The inositol polyphosphate 5-phosphatases selectively remove the phosphate from the 5-position of various phosphatidylinositols, which generate second messengers in response to extracellular signals. OCRL1 is a type II 5-phosphatase that is mutated in the oculocerebrorenal syndrome of Lowe (OCRL). OCRL is a rare X-linked disorder that is characterized in part by congenital cataracts, mental retardation, muscular hypotonia and renal tubular dysfunction. OCRL1 has a high affinity for phosphatidylinositol 4,5-bisphosphate as well as inositol 1,4,5-trisphosphate and inositol 1,3,4,5-tetrakisphosphate as substrates. OCRL1 is localized to the Golgi complex and is thought to be part of the trans-Golgi network (TGN), which suggests that OCRL1 plays a role in protein sorting and trafficking within the cell.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

Identification of novel OCRL isoforms associated with phenotypic differences between Dent disease-2 and Lowe syndrome.

Nephrology, dialysis, transplantation : official publication of the European Dialysis and Transplant Association - European Renal Association

Sakakibara N,Ijuin T,Horinouchi T,Yamamura T,Nagano C,Okada E,Ishiko S,Aoto Y,Rossanti R,Ninchoji T,Awano H,Nagase H,Minamikawa S,Tanaka R,Matsuyama T,Nagatani K,Kamei K,Jinnouchi K,Ohtsuka Y,Oka M,Araki Y,Tanaka T,Harada MS,Igarashi T,Kitahara H,Morisada N,Nakamura SI,Okada T,Iijima K,Nozu K

Tue Jan 25 00:00:00 UTC 2022

Novel pathogenic OCRL mutations and genotype-phenotype analysis of Chinese children affected by oculocerebrorenal syndrome: two cases and a literature review.

BMC medical genomics

Zhang Y,Deng L,Chen X,Hu Y,Chen Y,Chen K,Zhou J

Mon Sep 06 00:00:00 UTC 2021

Rab35 and glucocorticoids regulate APP and BACE1 trafficking to modulate Aβ production.

Cell death & disease

Zhuravleva V,Vaz-Silva J,Zhu M,Gomes P,Silva JM,Sousa N,Sotiropoulos I,Waites CL

Wed Dec 08 00:00:00 UTC 2021

Transcriptome analysis of neural progenitor cells derived from Lowe syndrome induced pluripotent stem cells: identification of candidate genes for the neurodevelopmental and eye manifestations.

Journal of neurodevelopmental disorders

Liu H,Barnes J,Pedrosa E,Herman NS,Salas F,Wang P,Zheng D,Lachman HM

Mon May 11 00:00:00 UTC 2020

Modeling the neuropsychiatric manifestations of Lowe syndrome using induced pluripotent stem cells: defective F-actin polymerization and WAVE-1 expression in neuronal cells.

Molecular autism

Barnes J,Salas F,Mokhtari R,Dolstra H,Pedrosa E,Lachman HM

Tue Dec 18 00:00:00 UTC 2018

Modeling the neuropsychiatric manifestations of Lowe syndrome using induced pluripotent stem cells: defective F-actin polymerization and WAVE-1 expression in neuronal cells.

Molecular autism

Barnes J,Salas F,Mokhtari R,Dolstra H,Pedrosa E,Lachman HM

Tue Dec 18 00:00:00 UTC 2018

Modeling the neuropsychiatric manifestations of Lowe syndrome using induced pluripotent stem cells: defective F-actin polymerization and WAVE-1 expression in neuronal cells.

Molecular autism

Barnes J,Salas F,Mokhtari R,Dolstra H,Pedrosa E,Lachman HM

Tue Dec 18 00:00:00 UTC 2018

Modeling the neuropsychiatric manifestations of Lowe syndrome using induced pluripotent stem cells: defective F-actin polymerization and WAVE-1 expression in neuronal cells.

Molecular autism

Barnes J,Salas F,Mokhtari R,Dolstra H,Pedrosa E,Lachman HM

Tue Dec 18 00:00:00 UTC 2018

Bioinformatics

Protein Aliases: Inositol polyphosphate 5-phosphatase OCRL; Inositol polyphosphate 5-phosphatase OCRL-1; Lowe oculocerebrorenal syndrome protein; OCRL-1; oculocerebrorenal syndrome of Lowe; Phosphatidylinositol 3,4,5-triphosphate 5-phosphatase; phosphatidylinositol polyphosphate 5-phosphatase

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Gene Aliases: 9530014D17Rik; BB143339; INPP5F; LOCR; NPHL2; OCRL; OCRL-1; OCRL1

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UniProt ID: (Human) Q01968, (Mouse) Q6NVF0

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Entrez Gene ID: (Human) 4952, (Rat) 317576, (Mouse) 320634

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Function(s)

phosphatidylinositol-4,5-bisphosphate 5-phosphatase activity GTPase activator activity protein binding Rac GTPase binding inositol-1,4,5-trisphosphate 5-phosphatase activity inositol-1,3,4,5-tetrakisphosphate 5-phosphatase activity inositol phosphate phosphatase activity molecular_function hydrolase activity

Process(es)

in utero embryonic development signal transduction biological_process cilium assembly phosphatidylinositol dephosphorylation lipid metabolic process phosphatidylinositol biosynthetic process regulation of GTPase activity positive regulation of GTPase activity inositol phosphate metabolic process regulation of small GTPase mediated signal transduction cell projection organization

Performance Guarantee

If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

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OCRL Polyclonal Antibody

OCRL Antibody (17695-1-AP) in IHC (P)

Product Details

17695-1-AP

Western Blot (WB)

Immunohistochemistry (IHC)

Immunohistochemistry (Paraffin) (IHC (P))

Immunocytochemistry (ICC/IF)

Immunoprecipitation (IP)

Species Reactivity

Published species

Host/Isotype

Class

Type

Immunogen

Conjugate

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

Shipping conditions

Product Specific Information

Target Information

Bioinformatics

Performance Guarantee

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