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Invitrogen
OCT1 (POU2F1) Polyclonal Antibody
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FIGURE: 1 / 1
OCT1 (POU2F1) Antibody (PA5-120490) in WB
Western blot analysis of OCT1 (POU2F1) in rat kidney. Samples were incubated with OCT1 (POU2F1) Polyclonal antibody (Product # PA5-120490) using a dilution of 1:1,000, followed by HRP Goat Anti-Rabbit IgG (H+L) at a dilution of 1:10,000. Lysates/proteins: 25 µg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 15s.
Product Details
PA5-120490
Product Specifications
Species Reactivity
Mouse,
Rat
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
Recombinant fusion protein containing a sequence corresponding to amino acids 15-290 of human POU2F1/OCT1 (NP_001185712.1).
Conjugate
Form
Liquid
Concentration
0.68 mg/mL
Purification
Affinity Chromatography
Storage buffer
PBS, pH 7.3, with 50% glycerol
Contains
0.02% sodium azide
Storage conditions
-20°C, Avoid Freeze/Thaw Cycles
Shipping conditions
Wet ice
RRID
AB_2914062
Product Specific Information
Positive test controls include: Rat kidney. The target is usually found in the following locations: Nucleus.
Immunogen sequence: NPSETSKPSM ESGDGNTGTQ TNGLDFQKQP VPVGGAISTA QAQAFLGHLH QVQLAGTSLQ AAAQSLNVQS KSNEESGDSQ QPSQPSQQPS VQAAIPQTQL MLAGGQITGL TLTPAQQQLL LQQAQAQAQL LAAAVQQHSA SQQHSAAGAT ISASAATPMT QIPLSQPIQI AQDLQQLQQL QQQNLNLQQF VLVHPTTNLQ PAQFIISQTP QGQQGLLQAQ NLLTQLPQQS QANLLQSQPS ITLTSQPATP TRTIAATPIQ TLPQSQSTPK RIDTPS
Target Information
OCT1 (POU2F1) is a ubiquitously expressed transcription factor that binds to the octamer motif (5'-ATTTGCAT-3') and activates the promoters of the genes for some small nuclear RNAs (snRNA) and of genes such as those for histone H2B and immunoglobulins. It modulates transcription transactivation by NR3C1, AR and PGR. In case of human herpes simplex virus (HSV) infection, POU2F1 forms a multiprotein-DNA complex with the viral transactivator protein VP16 and HCFC1 thereby enabling the transcription of the viral immediate early genes.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: NF-A1; Oct-1; Octamer-binding protein 1; Octamer-binding transcription factor 1; OTF-1; POU domain, class 2, transcription factor 1; ubiquitous transcription factor
Gene Aliases: 2810482H01Rik; NF-A1; Oct-1; Oct1; Otf-1; Otf1; Pou2f1
UniProt ID: (Rat) P31503, (Mouse) P25425
Entrez Gene ID: (Rat) 171068, (Mouse) 18986
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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