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Invitrogen
OCT1 (POU2F1) Monoclonal Antibody (YL15), eBioscience™
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FIGURE: 1 / 3
OCT1 (POU2F1) Antibody (14-9736-82) in ICC/IF
Immunofluorescent analysis of POU2F1 (green) in A549 cells (60 % confluency). The cells were fixed with 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 for 10 minutes at 37 C, and blocked with 10% Normal Donkey Serum in PBS for 1 hour at room temperature. Cells were then stained with OCT1 (POU2F1) Monoclonal Antibody, Unconjugated at 1.25 µg/mL Mouse IgG1 isotype... View More
Product Details
14-9736-82
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human
Host/Isotype
Mouse
/ IgG1, kappa
Class
Monoclonal
Type
Antibody
Clone
YL15
Immunogen
Recombinant full-length human OCT1 (POU2F1)
Conjugate
Form
Liquid
Concentration
0.5 mg/mL
Purification
Affinity chromatography
Storage buffer
PBS, pH 7.2
Contains
0.09% sodium azide
Storage conditions
4°C, do not freeze
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
AB_2802196
Product Specific Information
Description: This YL15 monoclonal antibody recognizes human OCT1 (POU2F1) and is predicted to recognize mouse POU2F1.
Applications Reported: This YL15 monoclonal antibody has been reported for use in western blot and immunocytochemical staining.
Applications Tested: This YL15 antibody has been tested by western blot on HeLa wild type and POU2F1 knock-out lysate and NTERA-2 lysate. This may be used at less than or equal to 4 µg/mL. This YL15 antibody has been tested by immunohistochemical staining of cultured A549 cells. This may be used at less than or equal to 1.25 µg/mL.
Target Information
OCT1 (POU2F1) is a ubiquitously expressed transcription factor that binds to the octamer motif (5'-ATTTGCAT-3') and activates the promoters of the genes for some small nuclear RNAs (snRNA) and of genes such as those for histone H2B and immunoglobulins. It modulates transcription transactivation by NR3C1, AR and PGR. In case of human herpes simplex virus (HSV) infection, POU2F1 forms a multiprotein-DNA complex with the viral transactivator protein VP16 and HCFC1 thereby enabling the transcription of the viral immediate early genes.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: NF-A1; Oct-1; Octamer-binding protein 1; Octamer-binding transcription factor 1; Octamer-binding transcription factor-1; OTF-1; POU domain, class 2, transcription factor 1
Gene Aliases: oct-1B; OCT1; OTF1; POU2F1
UniProt ID: (Human) P14859
Entrez Gene ID: (Human) 5451
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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