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OCT4 Monoclonal Antibody (1C4B6)

OCT4 Antibody in Immunohistochemistry (Paraffin) (IHC (P))

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OCT4 Antibody (60242-1-IG) in IHC (P)

Immunohistochemistry of paraffin-embedded human breast cancer tissue slide using 60242-1-Ig (OCT4 antibody) at dilution of 1:500 (under 40x lens) heat mediated antigen retrieved with Tris-EDTA buffer (pH 9).

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Product Details

60242-1-IG

Applications

Tested Dilution

Publications

Western Blot (WB)

1:5,000-1:20,000

View 23 publications 23 publications

Immunohistochemistry (IHC)

View 2 publications 2 publications

Immunohistochemistry (Paraffin) (IHC (P))

1:250-1:1,000

Immunocytochemistry (ICC/IF)

View 5 publications 5 publications

Product Specifications

Species Reactivity

Human, Mouse, Rat

Host/Isotype

Mouse / IgG1

Class

Monoclonal

Type

Antibody

Clone

1C4B6

Immunogen

OCT4 Fusion Protein Ag1794 (102-265 aa encoded by BC020712)

View immunogen

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

1 mg/mL

Purification

Protein A

Storage buffer

PBS, pH 7.3, with 50% glycerol

Contains

0.02% sodium azide

Storage conditions

-20°C

Shipping conditions

Wet ice (domestic); Dry ice (international)

Product Specific Information

Immunogen sequence: MCKLRPLLQ KWVEEADNNE NLQEICKAET LVQARKRKRT SIENRVRGNL ENLFLQCPKP TLQQISHIAQ QLGLEKDVVR VWFCNRRQKG KRSSSDYAQR EDFEAAGSPF SGGPVSFPLA PGPHFGTPGY GSPHFTALYS SVPFPEGEAF PPVSVTTLGS PMHSN (102-265 aa encoded by BC020712)

Target Information

POU5F1, also commonly known as Oct-4, is a maternally expressed octamer-binding protein that was the first transcription factor described for the early stages of development. The role of POU5F1 in embryonic development suggested that it might be useful in the creation of stem cells that might be useful in cell replacement therapies in the treatment of several degenerative diseases. Artificial stem cells, termed induced pluripotent stem (iPS) cells, can be created by expressing POU5F1 and the transcription factors Sox2, Klf4 and Lin28 along with c-Myc in mouse fibroblasts. More recently, experiments have demonstrated that iPS cells could be generated using expression plasmids expressing POU5F1, Sox2, KlfF4 and c-Myc, eliminating the need for virus introduction, thereby addressing a safety concern for potential use of iPS cells in regenerative medicine.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

NRP1 contributes to stemness and potentiates radioresistance via WTAP-mediated m6A methylation of Bcl-2 mRNA in breast cancer.

Apoptosis : an international journal on programmed cell death

Wang Y,Zhang L,Sun XL,Lu YC,Chen S,Pei DS,Zhang LS

Wed Feb 01 00:00:00 UTC 2023

Enhanced binding of β-catenin and β-TrCP mediates LMPt's anti-CSCs activity in colorectal cancer.

Biochemical pharmacology

Wang M,Wang X,Zhao W,Li Y,Cai M,Wang K,Xi X,Zhao C,Zhou H,Shao R,Xia G,Zhang Y,Zhao W

Thu Jun 01 00:00:00 UTC 2023

Generation of an induced pluripotent stem cell (iPSC) line (INNDSUi002-A) from a patient with riboflavin-responsive multiple acyl-CoA dehydrogenase deficiency.

Stem cell research

Zhang C,Wang D,Wang Y,Sun P,Zhao D,Zhao X,Zhao Y,Liu F,Xu J,Yan C

Thu Jun 01 00:00:00 UTC 2023

Integration of metabolomics and transcriptomics to reveal ferroptosis is involved in Tripterygium wilfordii polyglycoside tablet-induced testicular injury.

Journal of ethnopharmacology

Qin Z,Zhang G,Jiang S,Ning F,Zhao Z,Huang M,Jin J

Sat Mar 25 00:00:00 UTC 2023

CircNFIX stimulates the proliferation, invasion, and stemness properties of ovarian cancer cells by enhancing SH3RF3 mRNA stability via binding LIN28B.

The Kaohsiung journal of medical sciences

Yu XZ,Yang BW,Ao MY,Wu YK,Ye H,Wang RY,Xi MR,Hou MM

Wed Mar 01 00:00:00 UTC 2023

MicroRNA26a Overexpression Hastens Osteoblast Differentiation Capacity in Dental Stem Cells.

Cellular reprogramming

Kaufman S,Chang P,Pendleton E,Chandar N

Thu Jun 01 00:00:00 UTC 2023

Generation of a human induced pluripotent stem cell line (INNDSUi003-A) derived from patient with Becker muscular dystrophy (BMD).

Stem cell research

Ji X,Wang Y,Sun P,Wang D,Sun X,Zhang R,Xu J,Li W,Liu F,Yan C

Fri Jul 01 00:00:00 UTC 2022

Induced retinal pigment epithelial cells with anti-epithelial-to-mesenchymal transition ability delay retinal degeneration.

iScience

Tian H,Chen Z,Zhu X,Ou Q,Wang Z,Wu B,Xu JY,Jin C,Gao F,Wang J,Zhang J,Zhang J,Lu L,Xu GT

Fri Oct 21 00:00:00 UTC 2022

WTAP promotes oesophageal squamous cell carcinoma development by decreasing CPSF4 expression in an m⁶A-dependent manner.

Medical oncology (Northwood, London, England)

Luo Q,Zhan X,Kuang Y,Sun M,Dong F,Sun E,Chen B

Thu Sep 29 00:00:00 UTC 2022

Autism-associated protein POGZ controls ESCs and ESC neural induction by association with esBAF.

Molecular autism

Sun X,Cheng L,Sun Y

Wed Jun 01 00:00:00 UTC 2022

Autism-associated protein POGZ controls ESCs and ESC neural induction by association with esBAF.

Molecular autism

Sun X,Cheng L,Sun Y

Wed Jun 01 00:00:00 UTC 2022

RNA-seq and ATAC-seq analyses of multilineage differentiating stress enduring cells: Comparison with dermal fibroblasts.

Cell biology international

Mao XQ,Cheng Y,Zhang RZ,Liu YB,Li Y,Ge K,Jin HL

Thu Sep 01 00:00:00 UTC 2022

Establishment of an induced pluripotent stem cell (iPSC) line (INNDSUi001-A) from a healthy female Chinese Han.

Stem cell research

Wang D,Zhang R,Wang Y,Sun P,Ji X,Sun X,Xu J,Zhao D,Yan C,Liu F

Fri Jul 01 00:00:00 UTC 2022

Effect of the interference with DRP1 expression on the biological characteristics of glioma stem cells.

Experimental and therapeutic medicine

Zhang L,Cao H,Tao H,Yang J,Gong W,Hu Q

Thu Jul 01 00:00:00 UTC 2021

KDELR2 promotes breast cancer proliferation via HDAC3-mediated cell cycle progression.

Cancer communications (London, England)

Wei H,Ma W,Lu X,Liu H,Lin K,Wang Y,Ye Z,Sun L,Huang Z,Pan T,Zhou Z,Cheng EY,Zhang H,Gao P,Zhong X

Wed Sep 01 00:00:00 UTC 2021

Parthenolide inhibits the tumor characteristics of renal cell carcinoma.

International journal of oncology

Liu D,Han Y,Liu L,Ren X,Zhang H,Fan S,Qin T,Li L

Fri Jan 01 00:00:00 UTC 2021

Apatinib suppresses lung cancer stem-like cells by complex interplay between β-catenin signaling and mitochondrial ROS accumulation.

Cell death discovery

Zhu J,Li X,Liang C,Zhou X,Ge M,Chen Y,Jin J,Yin J,Xu H,Xie C,Zhong C

Wed May 12 00:00:00 UTC 2021

A novel lncRNA ROPM-mediated lipid metabolism governs breast cancer stem cell properties.

Journal of hematology & oncology

Liu S,Sun Y,Hou Y,Yang L,Wan X,Qin Y,Liu Y,Wang R,Zhu P,Teng Y,Liu M

Fri Oct 29 00:00:00 UTC 2021

Arl4c promotes the growth and drug resistance of pancreatic cancer by regulating tumor-stromal interactions.

iScience

Chen X,Zhang Y,Qian W,Han L,Li W,Duan W,Wu Z,Wang Z,Ma Q

Fri Dec 17 00:00:00 UTC 2021

PIWIL1 Drives Chemoresistance in Multiple Myeloma by Modulating Mitophagy and the Myeloma Stem Cell Population.

Frontiers in oncology

Wang Y,Yao L,Teng Y,Yin H,Wu Q

Fri Jan 28 00:00:00 UTC 2022

Gankyrin activates the hedgehog signalling to drive metastasis in osteosarcoma.

Journal of cellular and molecular medicine

Wang C,Jing J,Hu X,Yu S,Yao F,Li Z,Cheng L

Thu Jul 01 00:00:00 UTC 2021

Oxaliplatin resistance is enhanced by saracatinib via upregulation Wnt-ABCG1 signaling in hepatocellular carcinoma.

BMC cancer

Liao X,Song G,Xu Z,Bu Y,Chang F,Jia F,Xiao X,Ren X,Zhang M,Jia Q

Mon Jan 13 00:00:00 UTC 2020

Targeting SLC7A11 specifically suppresses the progression of colorectal cancer stem cells via inducing ferroptosis.

European journal of pharmaceutical sciences : official journal of the European Federation for Pharmaceutical Sciences

Xu X,Zhang X,Wei C,Zheng D,Lu X,Yang Y,Luo A,Zhang K,Duan X,Wang Y

Tue Sep 01 00:00:00 UTC 2020

Chlorogenic acid inhibits esophageal squamous cell carcinoma growth in vitro and in vivo by downregulating the expression of BMI1 and SOX2.

Biomedicine & pharmacotherapy = Biomedecine & pharmacotherapie

Zhan Y,Li R,Feng C,Li X,Huang S,Wang L,Liu Z,Jiang J,Han Y

Wed Jan 01 00:00:00 UTC 2020

lncRNA TPTEP1 inhibits stemness and radioresistance of glioma through miR‑106a‑5p‑mediated P38 MAPK signaling.

Molecular medicine reports

Tang T,Wang LX,Yang ML,Zhang RM

Tue Dec 01 00:00:00 UTC 2020

Tobacco smoke induced hepatic cancer stem cell-like properties through IL-33/p38 pathway.

Journal of experimental & clinical cancer research : CR

Xie C,Zhu J,Wang X,Chen J,Geng S,Wu J,Zhong C,Li X

Mon Jan 28 00:00:00 UTC 2019

Tobacco smoke induced hepatic cancer stem cell-like properties through IL-33/p38 pathway.

Journal of experimental & clinical cancer research : CR

Xie C,Zhu J,Wang X,Chen J,Geng S,Wu J,Zhong C,Li X

Mon Jan 28 00:00:00 UTC 2019

Cinobufotalin powerfully reversed EBV-miR-BART22-induced cisplatin resistance via stimulating MAP2K4 to antagonize non-muscle myosin heavy chain IIA/glycogen synthase 3β/β-catenin signaling pathway.

EBioMedicine

Liu Y,Jiang Q,Liu X,Lin X,Tang Z,Liu C,Zhou J,Zhao M,Li X,Cheng Z,Li L,Xie Y,Liu Z,Fang W

Tue Oct 01 00:00:00 UTC 2019

EBioMedicine

Liu Y,Jiang Q,Liu X,Lin X,Tang Z,Liu C,Zhou J,Zhao M,Li X,Cheng Z,Li L,Xie Y,Liu Z,Fang W

Tue Oct 01 00:00:00 UTC 2019

Sulforaphane Inhibits the Acquisition of Tobacco Smoke-Induced Lung Cancer Stem Cell-Like Properties via the IL-6/ΔNp63α/Notch Axis.

Theranostics

Xie C,Zhu J,Jiang Y,Chen J,Wang X,Geng S,Wu J,Zhong C,Li X,Meng Z

Tue Aug 25 00:00:00 UTC 2020

Bioinformatics

Protein Aliases: 3-Oct; 4-Oct; DADB-104B20.2; gp-9; HGNC:9221; MGC22487; NF-A3; Oct-3; Oct-4; Octamer-binding protein 3; Octamer-binding protein 4; Octamer-binding transcription factor 3; OTF-3; OTTHUMP00000221150; OTTHUMP00000221151; POU class 5 homeobox 1 transcript variant OCT4B1; POU class 5 homeobox 1 transcript variant OCT4B2; POU class 5 homeobox 1 transcript variant OCT4B3; POU class 5 homeobox 1 transcript variant OCT4B4; POU class 5 homeobox 1 transcript variant OCT4B5; POU class 5 homeobox 1 transcript variant OCT4B6; POU domain class 5 transcription factor 1; POU domain transcription factor OCT4; POU domain, class 5, transcription factor 1; pou-2; POU-type homeodomain-containing DNA-binding protein; pou2

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Gene Aliases: NF-A3; Oct-3; Oct-3/4; Oct-4; OCT3; Oct3/4; OCT4; OTF-3; Otf-4; OTF3; Otf3-rs7; Otf3g; OTF4; POU5F1

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UniProt ID: (Human) Q01860, (Mouse) P20263

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Entrez Gene ID: (Human) 5460, (Mouse) 18999, (Rat) 294562

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Function(s)

transcription regulatory region sequence-specific DNA binding RNA polymerase II transcription factor activity, sequence-specific DNA binding transcriptional repressor activity, RNA polymerase II transcription regulatory region sequence-specific binding DNA binding transcription factor activity, sequence-specific DNA binding protein binding ubiquitin protein ligase binding miRNA binding sequence-specific DNA binding poly(A) RNA binding RNA polymerase II core promoter proximal region sequence-specific DNA binding transcriptional activator activity, RNA polymerase II core promoter proximal region sequence-specific binding RNA polymerase II transcription coactivator activity chromatin binding transcription corepressor activity transcription factor binding chromatin DNA binding transcription regulatory region DNA binding protein heterodimerization activity POU domain binding HMG box domain binding

Process(es)

negative regulation of transcription from RNA polymerase II promoter mesodermal cell fate commitment endodermal cell fate commitment ectodermal cell fate commitment endodermal cell fate specification trophectodermal cell differentiation blastocyst growth BMP signaling pathway involved in heart induction transcription, DNA-templated regulation of transcription, DNA-templated transcription from RNA polymerase II promoter multicellular organism development response to organic substance regulation of gene expression stem cell population maintenance germ-line stem cell population maintenance response to retinoic acid somatic stem cell population maintenance positive regulation of catenin import into nucleus mRNA transcription from RNA polymerase II promoter cell fate commitment negative regulation of cell differentiation negative regulation of transcription, DNA-templated positive regulation of transcription, DNA-templated positive regulation of transcription from RNA polymerase II promoter negative regulation of calcium ion-dependent exocytosis stem cell differentiation positive regulation of protein kinase B signaling negative regulation of protein kinase B signaling positive regulation of SMAD protein import into nucleus cell fate commitment involved in formation of primary germ layer cardiac cell fate determination negative regulation of gene silencing by miRNA regulation of heart induction by regulation of canonical Wnt signaling pathway regulation of methylation-dependent chromatin silencing blastocyst development response to wounding anatomical structure morphogenesis regulation of asymmetric cell division response to cytokine

Performance Guarantee

If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

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OCT4 Monoclonal Antibody (1C4B6)

OCT4 Antibody (60242-1-IG) in IHC (P)

Product Details

60242-1-IG

Western Blot (WB)

Immunohistochemistry (IHC)

Immunohistochemistry (Paraffin) (IHC (P))

Immunocytochemistry (ICC/IF)

Species Reactivity

Host/Isotype

Class

Type

Clone

Immunogen

Conjugate

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

Shipping conditions

Product Specific Information

Target Information

Bioinformatics

Performance Guarantee

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