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OLIG3 Antibody (PA5-110060) in WB
Western blot analysis of OLIG3 in extracts of various cell lines. Samples were incubated with OLIG3 Polyclonal antibody (Product # PA5-110060) using a dilution of 1:3,000, followed by HRP Goat Anti-Rabbit IgG (H+L) at a dilution of 1:10,000. Lysates/proteins: 25 µg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s.
Product Details
PA5-110060
Product Specifications
Species Reactivity
Mouse,
Rat
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
Recombinant fusion protein containing a sequence corresponding to amino acids 163-272 of human OLIG3 (NP_786923.1).
Conjugate
Form
Liquid
Concentration
1.43 mg/mL
Purification
Affinity Chromatography
Storage buffer
PBS, pH 7.3, with 50% glycerol
Contains
0.01% thimerosal
Storage conditions
-20°C, Avoid Freeze/Thaw Cycles
Shipping conditions
Wet ice
RRID
AB_2855471
Product Specific Information
Immunogen sequence: TVGHSAGHPA HAANSVHPVH PILGGALSSG NASSPLSAAS LPAIGTIRPP HSLLKAPSTP PALQLGSGFQ HWAGLPCPCT ICQMPPPPHL SALSTANMAR LSAESKDLLK
Target Information
OLIG3 may determine the distinct specification program of class A neurons in the dorsal part of the spinal cord and suppress specification of class B neurons.
⚠WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause birth defects or other reproductive harm. For more information go to www.P65Warnings.ca.gov.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: basic helix-loop-helix domain-containing class B protein 7; bHLHb7; bHLHe20oligo3; Class B basic helix-loop-helix protein 7; Oligo3; oligodendrocyte transcription factor 2; Oligodendrocyte transcription factor 3; Oligodendrocyte-specific bHLH transcription factor 3
Gene Aliases: Bhlhb7; bHLHe20; Olig3
UniProt ID: (Mouse) Q6PFG8
Entrez Gene ID: (Mouse) 94222, (Rat) 293012
negative regulation of transcription from RNA polymerase II promoter
transcription, DNA-templated
regulation of transcription, DNA-templated
transcription from RNA polymerase II promoter
regulation of gene expression
spinal cord motor neuron cell fate specification
spinal cord motor neuron differentiation
spinal cord motor neuron migration
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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