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OLIG3 Antibody (MA5-42609) in WB
Western blot analysis of OLIG3 in extracts of U-251MG cells. Samples were incubated with OLIG3 Monoclonal antibody (Product # MA5-42609) using a dilution of 1:1,000, followed by HRP Goat Anti-Rabbit IgG (H+L) at a dilution of 1:10,000. Lysates/proteins: 25 µg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 10s.
Product Details
MA5-42609
Product Specifications
Species Reactivity
Human
Host/Isotype
Rabbit
/ IgG
Expression System
HEK293 cells
Class
Recombinant Monoclonal
Type
Antibody
Clone
9C1N3
Immunogen
A synthetic peptide corresponding to a sequence within amino acids 173-272 of human Olig3 (Q7RTU3).
Conjugate
Form
Liquid
Concentration
0.5 mg/mL
Purification
Affinity Chromatography
Storage buffer
PBS, pH 7.3, with 50% glycerol, 0.05% BSA
Contains
0.02% sodium azide
Storage conditions
-20°C, Avoid Freeze/Thaw Cycles
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
AB_2911750
Product Specific Information
Positive test controls include: U-251MG. The target is usually found in the following locations: Nucleus.
Immunogen sequence: HAANSVHPVH PILGGALSSG NASSPLSAAS LPAIGTIRPP HSLLKAPSTP PALQLGSGFQ HWAGLPCPCT ICQMPPPPHL SALSTANMAR LSAESKDLLK
Target Information
OLIG3 may determine the distinct specification program of class A neurons in the dorsal part of the spinal cord and suppress specification of class B neurons.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: bHLHb7; bHLHe20; bHLHe20oligo3; Class B basic helix-loop-helix protein 7; Class E basic helix-loop-helix protein 20; Oligo3; Oligodendrocyte transcription factor 3
Gene Aliases: BHLHB7; BHLHE20; OLIG3
UniProt ID: (Human) Q7RTU3
Entrez Gene ID: (Human) 167826
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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