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Invitrogen
OLIG3 Recombinant Rabbit Monoclonal Antibody (JE56-40)
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FIGURE: 1 / 4
OLIG3 Antibody (MA5-44904) in ICC/IF
Immunocytochemistry analysis of Olig3 in F9 cells (green). Formalin fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 10 minutes at room temperature and blocked with 1% Blocker BSA for 15 minutes at room temperature. Samples were incubated in Olig3 Monoclonal antibody (Product # MA5-44904) using a dilution of 1:50 for 1 hour at room temperature, washed with PBS followed by Alexa Fluor®488 Goat anti-Rabbit IgG secondary antibody at a dilution of 1:1,000. The nuclear counter stain is DAPI (blue).
Product Details
MA5-44904
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse
Host/Isotype
Rabbit
/ IgG
Expression System
HEK293 cells
Class
Recombinant Monoclonal
Type
Antibody
Clone
JE56-40
Immunogen
Synthetic peptide within human Olig3 aa 222-272.
Conjugate
Form
Liquid
Concentration
1 mg/mL
Purification
Protein A
Storage buffer
TBS, pH 7.4, with 40% glycerol, 0.05% BSA
Contains
0.05% sodium azide
Storage conditions
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
AB_2931360
Target Information
OLIG3 may determine the distinct specification program of class A neurons in the dorsal part of the spinal cord and suppress specification of class B neurons.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: basic helix-loop-helix domain-containing class B protein 7; bHLHb7; bHLHe20; bHLHe20oligo3; Class B basic helix-loop-helix protein 7; Class E basic helix-loop-helix protein 20; Oligo3; Oligodendrocyte transcription factor 3; Oligodendrocyte-specific bHLH transcription factor 3
Gene Aliases: BHLHB7; BHLHE20; OLIG3
UniProt ID: (Human) Q7RTU3, (Mouse) Q6PFG8
Entrez Gene ID: (Human) 167826, (Mouse) 94222
negative regulation of transcription from RNA polymerase II promoter
transcription, DNA-templated
regulation of transcription, DNA-templated
transcription from RNA polymerase II promoter
regulation of gene expression
spinal cord motor neuron cell fate specification
spinal cord motor neuron differentiation
spinal cord motor neuron migration
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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