Invitrogen

OPA1 Polyclonal Antibody

3 Published Figures

5 References

View all (17) OPA1 antibodies

Datasheet

Protocols

Questions & Answers

Datasheet

Protocols

Questions & Answers

Antibody Testing Data (4)
Published Figures (3)

OPA1 Antibody in Immunohistochemistry (Paraffin) (IHC (P))

FIGURE: 1 / 7

OPA1 Antibody (PA1-16991) in IHC (P)

Immunohistochemical analysis of OPA1 in prostatic smooth muscle and glandular epithelium. Samples were incubated in OPA1 polyclonal antibody (Product # PA1-16991). Human Prostate 40X magnification.

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Product Details

PA1-16991

Applications

Tested Dilution

Publications

Western Blot (WB)

2 µg/mL

View 5 publications 5 publications

Immunohistochemistry (Paraffin) (IHC (P))

2.5 µg/mL

Immunocytochemistry (ICC/IF)

Assay-Dependent

Product Specifications

Species Reactivity

Chicken, Human, Mouse, Pig, Rat, Zebrafish

Published species

Mouse, Pig

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

Synthetic peptide corresponding to residues 500-600 of human Opa1.

View immunogen

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

1 mg/mL

Purification

Antigen affinity chromatography

Storage buffer

PBS

Contains

0.02% sodium azide

Storage conditions

-20°C, Avoid Freeze/Thaw Cycles

Shipping conditions

Ambient (domestic); Wet ice (international)

AB_2158151

Product Specific Information

This antibody shares 100% sequence homology with primate, 94% with zebrafish.

Suggested positive control: human brain protein.

Target Information

OPA1 is a dynamin-related GTPase that is critical for the maintenance of mitochondrial morphology and mtDNA. The most commonly associated phenotype with OPA1 mutations is heterozygous optic atrophy, a heterozygous dominant trait that causes reduced visual clarity and sometimes blindness. The disease usually begins in childhood and increases in severity throughout the life of affected individuals. Usually, this phenotype is attributed to the degeneration of optic nerve fibers. Interestingly, the same type of nerve degeneration seems to be partially causative of certain schizophrenia characteristics. OPA1 dysfunction also seems to be implicated in this case; mitochondrial networks associated with critical nerves seem to link schizophrenia and OPA1. The dysfunction is associated with issues with apoptosis and normal cellular metabolic regulation, all regulated through OPA1.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

Antioxidants (Basel, Switzerland)

Tolomeo AM,Fabozzo A,Malvicini R,De Lazzari G,Bisaccia P,Gaburro G,Arcidiacono D,Notarangelo D,Caicci F,Zanella F,Marchesan M,Yannarelli G,Santovito G,Muraca M,Gerosa G

PA1-16991 was used in Immunocytochemistry, Immunohistochemistry (Paraffin), Western Blot to demonstrate that normothermic cardioplegia preserved mitochondria morphology, thus preventing oxidative stress and the subsequent inflammatory response.

Mon Feb 28 00:00:00 UTC 2022

Antioxidants (Basel, Switzerland)

Tolomeo AM,Fabozzo A,Malvicini R,De Lazzari G,Bisaccia P,Gaburro G,Arcidiacono D,Notarangelo D,Caicci F,Zanella F,Marchesan M,Yannarelli G,Santovito G,Muraca M,Gerosa G

Mon Feb 28 00:00:00 UTC 2022

Tau Deletion Prevents Cognitive Impairment and Mitochondrial Dysfunction Age Associated by a Mechanism Dependent on Cyclophilin-D.

Frontiers in neuroscience

Jara C,Cerpa W,Tapia-Rojas C,Quintanilla RA

Published figure using OPA1 polyclonal antibody (Product # PA1-16991) in Western Blot

Wed Mar 10 00:00:00 UTC 2021

Intracardiac administration of ephrinA1-Fc preserves mitochondrial bioenergetics during acute ischemia/reperfusion injury.

Life sciences

Torres MJ,McLaughlin KL,Renegar RH,Valsaraj S,Whitehurst KS,Sharaf OM,Sharma UM,Horton JL,Sarathy B,Parks JC,Brault JJ,Fisher-Wellman KH,Neufer PD,Virag JAI

PA1-16991 was used in Western Blotting to suggest that intracardiac injection of EphrinA1-Fc protects cardiac function by preserving cardiomyocyte structure and mitochondrial bioenergetics, thus emerging as a potential therapeutic strategy in I/R injury.

Sun Dec 15 00:00:00 UTC 2019

Genetic ablation of tau improves mitochondrial function and cognitive abilities in the hippocampus.

Redox biology

Jara C,Aránguiz A,Cerpa W,Tapia-Rojas C,Quintanilla RA

Published figure using OPA1 polyclonal antibody (Product # PA1-16991) in Western Blot

Sat Sep 01 00:00:00 UTC 2018

Quantitative proteomic and functional analysis of liver mitochondria from high fat diet (HFD) diabetic mice.

Molecular & cellular proteomics : MCP

Guo Y,Darshi M,Ma Y,Perkins GA,Shen Z,Haushalter KJ,Saito R,Chen A,Lee YS,Patel HH,Briggs SP,Ellisman MH,Olefsky JM,Taylor SS

PA1-16991 was used in western blot to use diabetic mice fed a high-fat diet to study disease-related changes in mitochondrial function and proteomics

Sun Dec 01 00:00:00 UTC 2013

Bioinformatics

Protein Aliases: dynamin-like 120 kDa protein, mitochondrial; Dynamin-like GTPase OPA1, mitochondrial; dynamin-like guanosine triphosphatase; Large GTP-binding protein; LargeG; mitochondrial dynamin-like GTPase; mitochondrial OPA1; opa1 {ECO:0000312|EMBL:AAI11072.1}; optic atrophy 1 (autosomal dominant); optic atrophy 1 homolog; optic atrophy 1-like protein; Optic atrophy protein 1; Optic atrophy protein 1 homolog; RN protein

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Gene Aliases: 1200011N24Rik; AI225888; AI847218; BERHS; fk62d06; KIAA0567; largeG; lilr3; MGM1; mKIAA0567; MTDPS14; NPG; NTG; OPA1; RCJMB04_1m16; wu:fb77a10; wu:fk62d06; zgc:92092

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UniProt ID: (Chicken) Q5F499, (Human) O60313, (Rat) Q2TA68, (Mouse) P58281, (Zebrafish) Q5U3A7

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Entrez Gene ID: (Chicken) 424900, (Human) 4976, (Rat) 171116, (Mouse) 74143, (Zebrafish) 492332

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Function(s)

GTPase activity GTP binding magnesium ion binding protein binding molecular_function nucleotide binding hydrolase activity microtubule binding lipid binding phosphatidic acid binding cardiolipin binding

Process(es)

mitochondrial genome maintenance neural tube closure apoptotic process mitochondrion organization inner mitochondrial membrane organization aging visual perception mitochondrial fusion metabolic process positive regulation of mitochondrial fusion positive regulation of neuron maturation negative regulation of release of cytochrome c from mitochondria cellular senescence positive regulation of dendrite development negative regulation of endoplasmic reticulum stress-induced intrinsic apoptotic signaling pathway negative regulation of intrinsic apoptotic signaling pathway response to stimulus mitochondrial fission dynamin polymerization involved in mitochondrial fission chordate embryonic development GTP metabolic process ventricular cardiac muscle cell development membrane fusion mitochondrion morphogenesis membrane tubulation axonal transport of mitochondrion

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OPA1 Polyclonal Antibody

OPA1 Antibody (PA1-16991) in IHC (P)

Product Details

PA1-16991

Western Blot (WB)

Immunohistochemistry (Paraffin) (IHC (P))

Immunocytochemistry (ICC/IF)

Species Reactivity

Published species

Host/Isotype

Class

Type

Immunogen

Conjugate

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

Shipping conditions

RRID

Product Specific Information

Target Information

Bioinformatics

Performance Guarantee

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