Invitrogen

OPA1 Monoclonal Antibody (1E8-1D9)

OPA1 Antibody in Immunocytochemistry (ICC/IF)

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OPA1 Antibody (MA5-16149) in ICC/IF

Immunofluorescence analysis of OPA1 was performed using 70% confluent log phase A-431 cells. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 15 minutes, and blocked with 2% BSA for 1 hour at room temperature. The cells were labeled with OPA1 Monoclonal Antibody (Product # MA5-16149) at 1:100 dilution in 0.1% BSA, incubated at 4 degree Celsius overnight and then labeled with Donkey anti-Mouse IgG (H+L) Highly Cross-Adsorbed Secondary Antibody, Alexa Fluor Plus 488 (Product # A32766) at... View More

OPA1 Antibody in Immunohistochemistry (Paraffin) (IHC (P))

Product Image Gallery

OPA1 Antibody — Altered expression of target protein upon cell treatment demonstrates antibody specificity. Western blot analysis of OPA1 using OPA1 Monoclonal Antibody (Product # MA5-16149) shows an increased expression of its lower isoform at 75 kDa in HeLa on CCCP treatment, in comparison to untreated HeLa cells, with predominant band at 110 kDa. {TM}

Product Details

MA5-16149

Applications

Tested Dilution

Publications

Western Blot (WB)

1:1,000

View 4 publications 4 publications

Immunohistochemistry (Paraffin) (IHC (P))

1:100

Immunocytochemistry (ICC/IF)

1:50

View 1 publication 1 publication

Product Specifications

Species Reactivity

Bovine, Hamster, Human, Mouse, Rat

Published species

Human, Mouse

Host/Isotype

Mouse / IgG1, kappa

Class

Monoclonal

Type

Antibody

Clone

1E8-1D9

Immunogen

Human OPA1.

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

1 mg/mL

Purification

Protein G

Storage buffer

PBS

Contains

0.02% sodium azide

Storage conditions

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

Shipping conditions

Ambient (domestic); Wet ice (international)

AB_11153569

Product Specific Information

In Western blot, multiple protein isoforms can be seen at approximately 90, 80 and 65 kDa.

Target Information

OPA1 is a dynamin-related GTPase that is critical for the maintenance of mitochondrial morphology and mtDNA. The most commonly associated phenotype with OPA1 mutations is heterozygous optic atrophy, a heterozygous dominant trait that causes reduced visual clarity and sometimes blindness. The disease usually begins in childhood and increases in severity throughout the life of affected individuals. Usually, this phenotype is attributed to the degeneration of optic nerve fibers. Interestingly, the same type of nerve degeneration seems to be partially causative of certain schizophrenia characteristics. OPA1 dysfunction also seems to be implicated in this case; mitochondrial networks associated with critical nerves seem to link schizophrenia and OPA1. The dysfunction is associated with issues with apoptosis and normal cellular metabolic regulation, all regulated through OPA1.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

Mitochondrial health index correlates with plasma circulating cell-free mitochondrial DNA in bipolar disorder.

Molecular psychiatry

Cordeiro RC,Lima CNC,Fries GR,Zunta-Soares G,Soares JC,Quevedo J,Scaini G

MA5-16149 was used in Western Blotting to evaluate the composite mitochondrial health index (MHI) in BD subjects and non-psychiatry controls.

Wed Nov 01 00:00:00 UTC 2023

Chemotherapy impairs skeletal muscle mitochondrial homeostasis in early breast cancer patients.

Journal of cachexia, sarcopenia and muscle

Mallard J,Hucteau E,Charles AL,Bender L,Baeza C,Pélissie M,Trensz P,Pflumio C,Kalish-Weindling M,Gény B,Schott R,Favret F,Pivot X,Hureau TJ,Pagano AF

MA5-16149 was used in Western Blotting to investigating overall skeletal muscle mitochondrial homeostasis in early breast cancer patients undergoing chemotherapy, including mitochondrial quantity, function, and dynamics.

Wed Jun 01 00:00:00 UTC 2022

MITOL-mediated DRP1 ubiquitylation and degradation promotes mitochondrial hyperfusion in a CMT2A-linked MFN2 mutant.

Journal of cell science

Das R,Kamal IM,Das S,Chakrabarti S,Chakrabarti O

MA5-16149 was used in Immunocytochemistry, Immunohistochemistry (Paraffin), Western Blot to provide a mechanistic insight into mitochondrial hyperfusion, while also reporting that mitofusin 2 (MFN2) can indirectly modulate DRP1 - an effect not shown previously.

Sat Jan 15 00:00:00 UTC 2022

Ubiquitin-mediated regulation of the E3 ligase GP78 by MGRN1 in trans affects mitochondrial homeostasis.

Journal of cell science

Mukherjee R,Chakrabarti O

MA5-16149 was used in western blot to determine how mitochondrial homeostasis is affected by ubiquitin-mediated regulation of E3 ligase GP78 by MGRN1 in trans

Mon Feb 15 00:00:00 UTC 2016

Mechanical ventilation triggers abnormal mitochondrial dynamics and morphology in the diaphragm.

Journal of applied physiology (Bethesda, Md. : 1985)

Picard M,Azuelos I,Jung B,Giordano C,Matecki S,Hussain S,White K,Li T,Liang F,Benedetti A,Gentil BJ,Burelle Y,Petrof BJ

MA5-16149 was used in western blot to study the effect of diaphragm contractile inactivity on mitochondrial function.

Fri May 01 00:00:00 UTC 2015

Bioinformatics

Protein Aliases: Dynamin-like 120 kDa protein, mitochondrial; Dynamin-like GTPase OPA1, mitochondrial; dynamin-like guanosine triphosphatase; Large GTP-binding protein; LargeG; mitochondrial dynamin-like GTPase; mitochondrial OPA1; opa1 {ECO:0000312|EMBL:AAI11072.1}; optic atrophy 1 (autosomal dominant); optic atrophy 1 homolog; optic atrophy 1-like protein; Optic atrophy protein 1; Optic atrophy protein 1 homolog; RN protein

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Gene Aliases: 1200011N24Rik; AI225888; AI847218; BERHS; BOS_951; KIAA0567; largeG; lilr3; MGM1; mKIAA0567; MTDPS14; NPG; NTG; OPA1

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UniProt ID: (Human) O60313, (Rat) Q2TA68, (Mouse) P58281

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Entrez Gene ID: (Bovine) 524142, (Human) 4976, (Rat) 171116, (Mouse) 74143, (Chinese hamster) 100751873

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Function(s)

magnesium ion binding GTPase activity protein binding GTP binding molecular_function nucleotide binding hydrolase activity

Process(es)

mitochondrial genome maintenance neural tube closure apoptotic process mitochondrion organization inner mitochondrial membrane organization aging visual perception mitochondrial fusion metabolic process positive regulation of mitochondrial fusion positive regulation of neuron maturation negative regulation of release of cytochrome c from mitochondria cellular senescence positive regulation of dendrite development negative regulation of endoplasmic reticulum stress-induced intrinsic apoptotic signaling pathway negative regulation of intrinsic apoptotic signaling pathway response to stimulus mitochondrial fission axonal transport of mitochondrion

Performance Guarantee

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OPA1 Monoclonal Antibody (1E8-1D9)

OPA1 Antibody (MA5-16149) in ICC/IF

Product Details

MA5-16149

Western Blot (WB)

Immunohistochemistry (Paraffin) (IHC (P))

Immunocytochemistry (ICC/IF)

Species Reactivity

Published species

Host/Isotype

Class

Type

Clone

Immunogen

Conjugate

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

Shipping conditions

RRID

Product Specific Information

Target Information

Bioinformatics

Performance Guarantee

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