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Invitrogen
OPA3 Polyclonal Antibody
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OPA3 Antibody (PA5-96892) in WB
Western blot analysis of OPA3 in extracts of various cell lines. Samples were incubated with OPA3 Polyclonal antibody (Product # PA5-96892) using a dilution of 1:1,000, followed by HRP Goat Anti-Rabbit IgG (H+L) at a dilution of 1:10,000. Lysates/proteins: 25 µg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.
Product Details
PA5-96892
Product Specifications
Species Reactivity
Human,
Mouse,
Rat
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
Recombinant fusion protein containing a sequence corresponding to amino acids 25-179 of human OPA3 (NP_0794121)
Conjugate
Form
Liquid
Concentration
2.05 mg/mL
Purification
Affinity Chromatography
Storage buffer
PBS, pH 7.3, with 50% glycerol
Contains
0.02% sodium azide
Storage conditions
-20°C, Avoid Freeze/Thaw Cycles
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
AB_2808694
Product Specific Information
Immunogen sequence: NRIKEAARRS EFFKTYICLP PAQLYHWVEM RTKMRIMGFR GTVIKPLNEE AAAELGAELL GEATIFIVGG GCLVLEYWRH QAQQRHKEEE QRAAWNALRD EVGHLALALE ALQAQVQAAP PQGALEELRT ELQEVRAQLC NPGRSASHAV PASKK; Positive Samples: Mouse eye, Rat eye; Cellular Location: Mitochondrion
Target Information
The mouse ortholog of this protein co-purifies with the mitochondrial inner membrane. Mutations in this gene have been shown to result in 3-methylglutaconic aciduria type III and autosomal dominant optic atrophy and cataract. Multiple transcript variants encoding different isoforms have been found for this gene.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: FLJ22187; FLJ25932; MGC75494; optic atrophy 3 (autosomal recessive, with chorea and spastic paraplegia); Optic atrophy 3 (Iraqi-Jewish 'optic atrophy plus'); Optic atrophy 3 protein; Optic atrophy 3 protein homolog
Gene Aliases: D630048P19Rik; Gm1425; Gm472; MGA3; OPA3
UniProt ID: (Human) Q9H6K4, (Mouse) Q505D7
Entrez Gene ID: (Human) 80207, (Mouse) 403187, (Rat) 308409
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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