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OPLAH Antibody (PA5-116543) in WB
Western blot analysis of OPLAH in extracts of various cell lines. Samples were incubated with OPLAH Polyclonal antibody (Product # PA5-116543) using a dilution of 1:3,000, followed by HRP Goat Anti-Rabbit IgG (H+L) at a dilution of 1:10,000. Lysates/proteins: 25 µg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s.
Product Details
PA5-116543
Product Specifications
Species Reactivity
Mouse
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
Recombinant fusion protein containing a sequence corresponding to amino acids 1119-1288 of human OPLAH (NP_0600401)
Conjugate
Form
Liquid
Concentration
0.54 mg/mL
Purification
Affinity chromatography
Storage buffer
PBS, pH 7.3, with 50% glycerol
Contains
0.01% thimerosal
Storage conditions
-20°C, Avoid Freeze/Thaw Cycles
Shipping conditions
Wet ice
RRID
AB_2901174
Product Specific Information
Positive Samples: K-562, Mouse brain, Mouse kidney, Mouse liver
Immunogen sequence: TVAGGAGAGP SWHGRSGVHS HMTNTRITDP EILESRYPVI LRRFELRRGS GGRGRFRGGD GVTRELLFRE EALLSVLTER RAFRPYGLHG GEPGARGLNL LIRKNGRTVN LGGKTSVTVY PGDVFCLHTP GGGGYGDPED PAPPPGSPPQ ALAFPEHGSV YEYRRAQEAV
Target Information
OPLAH catalyzes the cleavage of 5-oxo-L-proline to form L-glutamate coupled to the hydrolysis of ATP to ADP and inorganic phosphate. ATP + 5-oxo-L-proline + 2 H2O = ADP + phosphate + L-glutamate.
⚠WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause birth defects or other reproductive harm. For more information go to www.P65Warnings.ca.gov.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: 5-OPase; 5-oxo-L-prolinase; 5-oxoprolinase; Pyroglutamase
Gene Aliases: 1700010G02Rik; Oplah
UniProt ID: (Mouse) Q8K010
Entrez Gene ID: (Mouse) 75475
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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