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ORC2 Antibody (MA5-17023) in ICC/IF
Immunofluorescence analysis of ORC2 was performed using HeLa cells. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 15 minutes, and blocked with 2% BSA for 1 hour at room temperature. The cells were labeled with ORC2 Monoclonal Antibody (Product # MA5-17023) at 1:200 dilution in 0.1% BSA and incubated overnight at 4 degree and then labeled with Goat anti-Rat IgG (H+L) Cross-Adsorbed Secondary Antibody, Alexa Fluor 488 (Product # A-11006) at a dilution of 1:2000 for 45 minutes at room ... View More
Product Details
MA5-17023
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human
Host/Isotype
Rat
/ IgG2a
Class
Monoclonal
Type
Antibody
Clone
3G6
Immunogen
Full length protein (Human): ORC2 (hsORC)
Conjugate
Form
Liquid
Concentration
1 mg/mL
Purification
Protein G
Storage buffer
PBS
Contains
0.09% sodium azide
Storage conditions
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
AB_2538495
Product Specific Information
MA5-17023 reacts with ORC2 in human samples.
MA5-17023 has been successfully used in immunoprecipitation and Western blot applications.
The MA5-17023 immunogen is ORC2 (hsORC) produced in P3-x63Ag.8.653 cells.
Target Information
The protein encoded by this gene belongs to the annexin family of calcium-dependent phospholipid binding proteins some of which have been implicated in membrane-related events along exocytotic and endocytotic pathways. Annexin 5 is a phospholipase A2 and protein kinase C inhibitory protein with calcium channel activity and a potential role in cellular signal transduction, inflammation, growth and differentiation. Annexin 5 has also been described as placental anticoagulant protein I, vascular anticoagulant-alpha, endonexin II, lipocortin V, placental protein 4 and anchorin CII. The gene spans 29 kb containing 13 exons, and encodes a single transcript of approximately 1.6 kb and a protein product with a molecular weight of about 35 kDa.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: origin recognition complex protein 2 homolog; Origin recognition complex subunit 2; origin recognition complex, subunit 2 homolog
Gene Aliases: ORC2; ORC2L
UniProt ID: (Human) Q13416
Entrez Gene ID: (Human) 4999
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