Invitrogen

OSBP Polyclonal Antibody

OSBP Antibody in Immunohistochemistry (Paraffin) (IHC (P))

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OSBP Antibody (PA5-30110) in IHC (P)

Immunohistochemical analysis of paraffin-embedded H441 xenograft, using OSBP (Product # PA5-30110) antibody at 1:100 dilution. Antigen Retrieval: EDTA based buffer, pH 8.0, 15 min.

OSBP Antibody in Immunocytochemistry (ICC/IF)

Product Image Gallery

OSBP Antibody — Effects of OSBP depletion on hPro-CpepSfGFP and CpepSfGFP. (A) OSBP immunostaining is concentrated in puncta that colocalize at the TGN with Golgin 97 (left) and are closely apposed to proinsulin (right). SfGFP mainly marks insulin granules and overlaps proinsulin (cyan) in young granules. Specificity of OSBP staining documented in Supplemental Figure S6. (B) Steady-state levels of both hPro-CpepSfGFP and CpepSfGFP are significantly decreased in OSBP knockdown cells as compared with control knockdown cells and are not restored by exogenous cholesterol. Quantification from Western blots; n = 4. (C) Endocytic uptake of lysosomal inhibitors partially restores both hPro-CpepSfGFP (hPro) and CpepSfGFP (Cpep) and increases the lysosomal SfGFP degradation product (Lys) in OSBP-depleted samples. The included image shows a representative Western blot (noncontiguous lanes are from the same blot). Quantification from Western blots; n = 4. (D) Images showing distinct separation of SfGFP fluorescence and Golgin97 immunostaining in control and ABCG1 knockdowns but increased overlap in OSBP knockdowns. Accompanying plot shows Manders’ overlap; control cells, # = 26; OSBP KD cells, # = 31. Data are presented as mean ± SEM; p values are determined by Student’s t test; *, p < 0.05; **, p < 0.01; ***, p < 0.001; ****, p < 0.0001. {KD}

Product Details

PA5-30110

Applications

Tested Dilution

Publications

Western Blot (WB)

1:500-1:3,000

Immunohistochemistry (Paraffin) (IHC (P))

1:100-1:1,000

Immunocytochemistry (ICC/IF)

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Product Specifications

Species Reactivity

Human, Mouse

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

Recombinant protein encompassing a sequence within the center region of human OSBP. The exact sequence is proprietary.

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

0.76 mg/mL

Purification

Antigen affinity chromatography

Storage buffer

0.1M tris glycine, pH 7, with 20% glycerol

Contains

0.01% thimerosal

Storage conditions

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

Shipping conditions

Ambient (domestic); Wet ice (international)

AB_2547584

Product Specific Information

Recommended positive controls: 293T, A431, Jurkat, Raji, mouse brain.

Predicted reactivity: Mouse (97%), Rat (98%), Rabbit (97%), Rhesus Monkey (99%), Bovine (91%).

Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.

Target Information

Oxysterol binding protein is an intracellular protein that is believed to transport sterols from lysosomes to the nucleus where the sterol down-regulates the genes for the LDL receptor, HMG-CoA reductase, and HMG synthetase.

⚠WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause birth defects or other reproductive harm. For more information go to www.P65Warnings.ca.gov.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

Control of insulin granule formation and function by the ABC transporters ABCG1 and ABCA1 and by oxysterol binding protein OSBP.

Molecular biology of the cell

Hussain SS,Harris MT,Kreutzberger AJB,Inouye CM,Doyle CA,Castle AM,Arvan P,Castle JD

Published figure using OSBP polyclonal antibody (Product # PA5-30110) in Immunofluorescence

Tue May 15 00:00:00 UTC 2018

Bioinformatics

Protein Aliases: HGNC:8503; Oxysterol-binding protein 1

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Gene Aliases: 1110018F06Rik; AW559088; Kiaa4220; mKIAA4220; OSBP; OSBP1

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UniProt ID: (Human) P22059, (Mouse) Q3B7Z2

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Entrez Gene ID: (Human) 5007, (Mouse) 76303

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Function(s)

protein binding oxysterol binding sterol transporter activity protein domain specific binding phosphatidylinositol-4-phosphate binding lipid binding

Process(es)

transport lipid transport positive regulation of growth of symbiont in host sterol transport

Performance Guarantee

If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

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OSBP Polyclonal Antibody

OSBP Antibody (PA5-30110) in IHC (P)

Product Details

PA5-30110

Western Blot (WB)

Immunohistochemistry (Paraffin) (IHC (P))

Immunocytochemistry (ICC/IF)

Species Reactivity

Host/Isotype

Class

Type

Immunogen

Conjugate

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

Shipping conditions

RRID

Product Specific Information

Target Information

Bioinformatics

Performance Guarantee

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