Proteintech

OSBPL2 Polyclonal Antibody

OSBPL2 Antibody in Immunohistochemistry (Paraffin) (IHC (P))

FIGURE: 1 / 7

OSBPL2 Antibody (14751-1-AP) in IHC (P)

Immunohistochemistry of paraffin-embedded human ovary tumor using 14751-1-AP (OSBPL2 antibody) at dilution of 1:100 (under 40x lens).

OSBPL2 Antibody in Immunoprecipitation (IP)

Product Image Gallery

Product Details

14751-1-AP

Applications

Tested Dilution

Publications

Western Blot (WB)

1:500-1:2,000

View 7 publications 7 publications

Immunohistochemistry (Paraffin) (IHC (P))

1:20-1:200

Immunocytochemistry (ICC/IF)

View 3 publications 3 publications

Immunoprecipitation (IP)

0.5-4.0 µg

ChIP assay (ChIP)

Assay-dependent

View 1 publication 1 publication

Miscellaneous PubMed (MISC)

View 1 publication 1 publication

Product Specifications

Species Reactivity

Human, Mouse

Published species

Human

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

OSBPL2 Fusion Protein Ag6496 (128-480 aa encoded by BC000296)

View immunogen

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

0.17 mg/mL

Purification

Antigen affinity chromatography

Storage buffer

PBS, pH 7.3, with 50% glycerol

Contains

0.02% sodium azide

Storage conditions

-20°C

Shipping conditions

Wet ice (domestic); Dry ice (international)

Product Specific Information

Immunogen sequence: ERMQSVAAF AVSAVASQWE RTGKPFNPLL GETYELIRED LGFRFISEQV SHHPPISAFH SEGLNHDFLF HGSIYPKLKF WGKSVEAEPR GTITLELLKH NEAYTWTNPT CCVHNVIIGK LWIEQYGTVE ILNHRTGHKC VLHFKPCGLF GKELHKVEGH IQDKNKKKLF MIYGKWTECL WGIDPVSYES FKKQERRGDH LRKAKLDEDS GKADSDVADD VPVAQETVQV IPGSKLLWRI NTRPPNSAQM YNFTSFTVSL NELETGMEKT LPPTDCRLRP DIRGMENGNM DLASQEKERL EEKQREARRE RAKEEAEWQT RWFYPGNNPY TGTPDWLYAG DYFERNFSDC PDIY (128-480 aa encoded by B C000296)

Target Information

This gene encodes a member of the oxysterol-binding protein (OSBP) family, a group of intracellular lipid receptors. Most members contain an N-terminal pleckstrin homology domain and a highly conserved C-terminal OSBP-like sterol-binding domain, although some members contain only the sterol-binding domain. This encoded protein contains only the sterol-binding domain. In vitro studies have shown that the encoded protein can bind strongly to phosphatic acid and weakly to phosphatidylinositol 3-phosphate, but cannot bind to 25-hydroxycholesterol. The protein associates with the Golgi apparatus. Transcript variants encoding different isoforms have been described.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

Mutations in OSBPL2 cause hearing loss associated with primary cilia defects via sonic hedgehog signaling.

JCI insight

Shi H,Wang H,Zhang C,Lu Y,Yao J,Chen Z,Xing G,Wei Q,Cao X

Tue Feb 22 00:00:00 UTC 2022

OSBPL2 mutations impair autophagy and lead to hearing loss, potentially remedied by rapamycin.

Autophagy

Koh YI,Oh KS,Kim JA,Noh B,Choi HJ,Joo SY,Rim JH,Kim HY,Kim DY,Yu S,Kim DH,Lee SG,Jung J,Choi JY,Gee HY

Tue Nov 01 00:00:00 UTC 2022

OSBPL2 mutations impair autophagy and lead to hearing loss, potentially remedied by rapamycin.

Autophagy

Koh YI,Oh KS,Kim JA,Noh B,Choi HJ,Joo SY,Rim JH,Kim HY,Kim DY,Yu S,Kim DH,Lee SG,Jung J,Choi JY,Gee HY

Tue Nov 01 00:00:00 UTC 2022

New label-free methods for protein relative quantification applied to the investigation of an animal model of Huntington Disease.

PloS one

Cozzolino F,Landolfi A,Iacobucci I,Monaco V,Caterino M,Celentano S,Zuccato C,Cattaneo E,Monti M

Mon Oct 26 00:00:00 UTC 2020

ORP2 Delivers Cholesterol to the Plasma Membrane in Exchange for Phosphatidylinositol 4, 5-Bisphosphate (PI(4,5)P₂).

Molecular cell

Wang H,Ma Q,Qi Y,Dong J,Du X,Rae J,Wang J,Wu WF,Brown AJ,Parton RG,Wu JW,Yang H

Thu Feb 07 00:00:00 UTC 2019

ORP2 Delivers Cholesterol to the Plasma Membrane in Exchange for Phosphatidylinositol 4, 5-Bisphosphate (PI(4,5)P₂).

Molecular cell

Wang H,Ma Q,Qi Y,Dong J,Du X,Rae J,Wang J,Wu WF,Brown AJ,Parton RG,Wu JW,Yang H

Thu Feb 07 00:00:00 UTC 2019

Niemann-Pick Type C Disease Reveals a Link between Lysosomal Cholesterol and PtdIns(4,5)P₂ That Regulates Neuronal Excitability.

Cell reports

Vivas O,Tiscione SA,Dixon RE,Ory DS,Dickson EJ

Tue May 28 00:00:00 UTC 2019

OSBPL2 deficiency upregulate SQLE expression increasing intracellular cholesterol and cholesteryl ester by AMPK/SP1 and SREBF2 signalling pathway.

Experimental cell research

Zhang C,Zhang H,Zhang M,Lin C,Wang H,Yao J,Wei Q,Lu Y,Chen Z,Xing G,Cao X

Tue Oct 15 00:00:00 UTC 2019

Molecular and cellular endocrinology