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Invitrogen
OTUB2 Recombinant Rabbit Monoclonal Antibody (139)
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FIGURE: 1 / 1
OTUB2 Antibody (MA5-29460) in ICC/IF
Immunofluorescence staining of Human OTUB2 in A549 cells. Cells were fixed with 4% PFA, permeabilzed with 0.3% Triton X-100 in PBS, blocked with 10% serum, and incubated with OTUB2 Recombinant Rabbit Monoclonal Antibody (139) (Product # MA5-29460, 1:60) at 4°C overnight. Then cells were stained with the Alexa Fluor® 549-conjugated Goat Anti-rabbit IgG secondary antibody (red). Positive staining was localized to cytoplasm and nucleus.
Product Details
MA5-29460
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human
Host/Isotype
Rabbit
/ IgG
Expression System
HEK293 cells
Class
Recombinant Monoclonal
Type
Antibody
Clone
139
Immunogen
Recombinant Human OTUB2 Protein (Met1-His234)
Conjugate
Form
Liquid
Concentration
1 mg/mL
Purification
Protein A
Storage buffer
PBS
Contains
no preservative
Storage conditions
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
AB_2785321
Product Specific Information
This product is preservative free. It is recommended to add sodium azide to avoid contamination (final concentration 0.05%-0.1%).
Recombinant rabbit monoclonal antibodies are produced using in vitro expression systems. The expression systems are developed by cloning in the specific antibody DNA sequences from immunoreactive rabbits. Then, individual clones are screened to select the best candidates for production. The advantages of using recombinant rabbit monoclonal antibodies include: better specificity and sensitivity, lot-to-lot consistency, animal origin-free formulations, and broader immunoreactivity to diverse targets due to larger rabbit immune repertoire.
This antibody has specificity for Human OTUB2.
Target Information
Otubains are deubiquitylating cysteine proteases that belong to the ovarian tumor protein superfamily. Like other DUBs, otubains cleave proteins precisely at the ubiquitin -protein bond.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: Deubiquitinating enzyme OTUB2; FLJ21916; MGC3102; OTU domain, ubiquitin aldehyde binding 2; OTU domain-containing ubiquitin aldehyde-binding protein 2; Otubain-2; Ubiquitin thioesterase OTUB2; ubiquitin-specific protease otubain 2; Ubiquitin-specific-processing protease OTUB2
Gene Aliases: C14orf137; OTB2; OTU2; OTUB2
UniProt ID: (Human) Q96DC9
Entrez Gene ID: (Human) 78990
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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