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Bethyl Laboratories
OTULIN Polyclonal Antibody
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FIGURE: 1 / 2
OTULIN Antibody (A305-821A-T) in WB
Detection of human and mouse OTULIN by western blot. Samples: Whole cell lysate (50 µg) from HeLa, HEK293T, U2OS, mouse TCMK-1, and mouse NIH 3T3 cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-OTULIN antibody A305-821A-M (lot A305-821A-M-1) used for WB at 1:1000. Detection: Chemiluminescence with an exposure time of 10 seconds.
Product Details
A305-821A-T
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
A region between residue 75 and 125 of human Protein FAM105B.
Conjugate
Form
Liquid
Concentration
1 mg/mL
Purification
Antigen affinity chromatography
Storage buffer
phosphate/tris citrate, pH 7.0 to 8.0
Contains
0.09% sodium azide
Storage conditions
4°C
Shipping conditions
Wet ice
Target Information
Otulin is a deubiquitinase that specifically removes linear ('Met-1'-linked) polyubiquitin chains to substrates and acts as a regulator of angiogenesis and innate immune response. Required during angiogenesis, craniofacial and neuronal development by regulating the canonical Wnt signaling together with the LUBAC complex. Acts as a negative regulator of NF-kappa-B by regulating the activity of the LUBAC complex. OTULIN function is mainly restricted to homeostasis of the LUBAC complex: acts by removing 'Met-1'-linked autoubiquitination of the LUBAC complex, thereby preventing inactivation of the LUBAC complex. Acts as a key negative regulator of inflammation by restricting spontaneous inflammation and maintaining immune homeostasis. In myeloid cell, required to prevent unwarranted secretion of cytokines leading to inflammation and autoimmunity by restricting linear polyubiquitin formation. Plays a role in innate immune response by restricting linear polyubiquitin formation on LUBAC complex in response to NOD2 stimulation, probably to limit NOD2-dependent proinflammatory signaling.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: Deubiquitinating enzyme otulin; family with sequence similarity 105, member B; gumby; OTU domain-containing deubiquitinase with linear linkage specificity; protein FAM105B; Ubiquitin thioesterase Gumby; Ubiquitin thioesterase otulin
Gene Aliases: C79097; FAM105B; GUM; m3Sapc; m7-1Sapc; OTULIN
UniProt ID: (Human) Q96BN8, (Mouse) Q3UCV8
Entrez Gene ID: (Human) 90268, (Mouse) 432940
angiogenesis
sprouting angiogenesis
immune system process
proteolysis
Wnt signaling pathway
negative regulation of NF-kappaB transcription factor activity
innate immune response
negative regulation of inflammatory response
canonical Wnt signaling pathway
nucleotide-binding oligomerization domain containing 2 signaling pathway
protein linear deubiquitination
regulation of tumor necrosis factor-mediated signaling pathway
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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