OriGene

OTX2 Monoclonal Antibody (OTI2B3), TrueMAB™

View all (23) OTX2 antibodies

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Datasheet
Protocols
Questions & Answers
Datasheet
Protocols
Questions & Answers

Product Details

CF809510

Applications
Tested Dilution
Publications

Western Blot (WB)

1:2,000
-

Immunohistochemistry (Paraffin) (IHC (P))

1:500
-
Product Specifications

Species Reactivity

Human, Mouse, Rat

Host/Isotype

Mouse / IgG2b

Class

Monoclonal

Type

Antibody

Clone

OTI2B3

Immunogen

Full length human recombinant protein of human OTX2 produced in E.coli.
View immunogen

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Lyophilized

Concentration

1 mg/mL

Purification

Affinity chromatography

Storage buffer

PBS, pH 7.3, with 8% trehalose

Contains

no preservative

Storage conditions

-20°C, Avoid Freeze/Thaw Cycles

Shipping conditions

Ambient (domestic); Wet ice (international)

Product Specific Information

For reconstitution, we recommend adding 100 µL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiments, we strongly recommend performing another round of desalting. (Zeba Spin Desalting Columns, 7KMWCO, 0.5 mL, Product # 89882)

Target Information

OTX2 is a 289 amino acid containing protein encoding a member of the bicoid sub-family of homeodomain-containing transcription factors. It has two known alternative splice variants with distinct isoforms though presence of other isoforms cannot be excluded. The protein is an early marker of endodermal differentiation of embryonic stem cells and may play a role in the development of the brain and the sensory organs. The protein may bind to BCD target sequence (BTS): 5-'TCTAATCCC-3'. Defects in OTX2 are the cause of microphthalmia syndromic type 5 (MCOPS5).

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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Bioinformatics

Protein Aliases: homeobox Otx2; Homeobox protein OTX2; Orthodenticle homolog 2; OTX

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Gene Aliases: CPHD6; E130306E05Rik; MCOPS5; OTX2

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UniProt ID: (Human) P32243

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Entrez Gene ID: (Human) 5015, (Rat) 305858, (Mouse) 18424

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Function(s)
RNA polymerase II core promoter proximal region sequence-specific DNA binding transcriptional activator activity, RNA polymerase II core promoter proximal region sequence-specific binding protein binding eukaryotic initiation factor 4E binding DNA binding transcription factor activity, sequence-specific DNA binding sequence-specific DNA binding protein heterodimerization activity
Process(es)
regulation of transcription, DNA-templated multicellular organism development transcription from RNA polymerase II promoter protein complex assembly axon guidance regulation of smoothened signaling pathway forebrain development midbrain development positive regulation of embryonic development regulation of fibroblast growth factor receptor signaling pathway positive regulation of transcription, DNA-templated positive regulation of transcription from RNA polymerase II promoter dopaminergic neuron differentiation primitive streak formation positive regulation of gastrulation cell fate specification regulation of transcription from RNA polymerase II promoter central nervous system development endoderm development organ morphogenesis anterior/posterior pattern specification dorsal/ventral pattern formation metencephalon development cell differentiation neuron differentiation somite rostral/caudal axis specification inner ear morphogenesis eye photoreceptor cell fate commitment cell fate commitment neuron fate commitment neuron fate determination diencephalon morphogenesis anatomical structure development
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