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          Clicking the images or links will redirect you to a website hosted by BenchSci that provides third-party scientific content. Neither the content nor the BenchSci technology and processes for selection have been evaluated by us; we are providing them as-is and without warranty of any kind, including for use or application of the Thermo Fisher Scientific products presented.

          • Primary Antibodies ›
          • Occludin Antibodies

          Invitrogen

          Occludin Polyclonal Antibody

          Advanced Verification
          51 Published Figures
          95 References
          View all (57) Occludin antibodies

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          Datasheet
          Protocols
          Questions & Answers
          Datasheet
          Protocols
          Questions & Answers

          Cite Occludin Polyclonal Antibody

          • Antibody Testing Data (5)
          • Published Figures (51)
          • Advanced Verification (2)
          Occludin Antibody in Immunocytochemistry (ICC/IF)
          Group 53 Created with Sketch.
          Occludin Antibody in Immunocytochemistry (ICC/IF)
          Group 53 Created with Sketch.

          FIGURE: 1 / 58

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          Occludin Antibody (40-4700) in ICC/IF

          Immunofluorescent analysis of occludin Antibody was done on 90% confluent log phase CACO2 cells. The cells were fixed with 4% paraformaldehyde for 15 minutes, permeabilized with 0.25% Triton™ X-100 for 10 minutes, and blocked with 5% BSA for 1 hour at room temperature. The cells were labeled with Occludin Antibody (Product # 40-4700) at 1µg/mL in 1% BSA and incubated for 3 hours at room temperature and then labeled with Alexa Fluor 488 Goat Anti-Rabbit IgG Secondary Antibody (Product # A-11008) at a dilution of 1:400 for 45 minutes at room... View More {{ $ctrl.currentElement.advancedVerification.fullName }} validation info. View more
          Published figure supplied by benchsci-logo
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          View Product

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          Occludin Antibody in Immunocytochemistry (ICC/IF)
          Occludin Antibody in Immunohistochemistry (Paraffin) (IHC (P))
          Occludin Antibody in Immunohistochemistry (Paraffin) (IHC (P))
          Occludin Antibody in Western Blot (WB)
          Occludin Antibody in Western Blot (WB)
          Occludin Antibody in Immunohistochemistry (IHC)
          Occludin Antibody in Immunohistochemistry (IHC)
          Occludin Antibody in Immunocytochemistry (ICC/IF)
          Occludin Antibody in Immunocytochemistry (ICC/IF)
          Occludin Antibody in Immunocytochemistry (ICC/IF)
          Occludin Antibody in Immunocytochemistry (ICC/IF)
          Occludin Antibody in Immunocytochemistry (ICC/IF)
          Occludin Antibody in Immunocytochemistry (ICC/IF)
          Occludin Antibody in Western Blot, Immunocytochemistry (WB, ICC/IF)
          Occludin Antibody in Immunocytochemistry (ICC/IF)
          Occludin Antibody in Western Blot, Immunocytochemistry (WB, ICC/IF)
          Occludin Antibody in Western Blot, Immunocytochemistry, Immunoprecipitation (WB, ICC/IF, IP)
          Occludin Antibody in Western Blot, Immunohistochemistry (Paraffin) (WB, IHC (P))
          Occludin Antibody in Immunohistochemistry (Frozen) (IHC (F))
          Occludin Antibody in Immunohistochemistry (Frozen) (IHC (F))
          Occludin Antibody in Immunohistochemistry (Frozen) (IHC (F))
          Occludin Antibody in Western Blot, Immunohistochemistry (WB, IHC)
          Occludin Antibody in Immunohistochemistry (IHC)
          Occludin Antibody in Western Blot, Immunohistochemistry (WB, IHC)
          Occludin Antibody in Immunohistochemistry (IHC)
          Occludin Antibody in Immunohistochemistry (IHC)
          Occludin Antibody in Immunohistochemistry (IHC)
          Occludin Antibody in Immunohistochemistry (IHC)
          Occludin Antibody in Immunohistochemistry (IHC)
          Occludin Antibody in Immunohistochemistry (IHC)
          Occludin Antibody in Immunohistochemistry (IHC)
          Occludin Antibody in Immunohistochemistry (IHC)
          Occludin Antibody in Western Blot, Immunohistochemistry (WB, IHC)
          Occludin Antibody in Western Blot (WB)
          Occludin Antibody in Western Blot (WB)
          Occludin Antibody in Western Blot (WB)
          Occludin Antibody in Western Blot (WB)
          Occludin Antibody in Western Blot (WB)
          Occludin Antibody in Western Blot (WB)
          Occludin Antibody in Western Blot (WB)
          Occludin Antibody in Western Blot (WB)
          Occludin Antibody in Western Blot (WB)
          Occludin Antibody in Western Blot (WB)
          Occludin Antibody in Western Blot (WB)
          Occludin Antibody in Western Blot (WB)
          Occludin Antibody in Western Blot (WB)
          Occludin Antibody in Western Blot (WB)
          Occludin Antibody in Western Blot (WB)
          Occludin Antibody in Western Blot (WB)
          Occludin Antibody in Western Blot (WB)
          Occludin Antibody in Western Blot (WB)
          Occludin Antibody in Western Blot (WB)
          Occludin Antibody in Western Blot (WB)
          Occludin Antibody in Western Blot (WB)
          Occludin Antibody in Western Blot (WB)
          Occludin Antibody in Western Blot (WB)
          Occludin Antibody
          Occludin Antibody

          Product Details

          40-4700

          Applications
          Tested Dilution
          Publications

          Western Blot (WB)

          1-3 µg/mL
          View 44 publications 44 publications

          Immunohistochemistry (IHC)

          -
          View 25 publications 25 publications

          Immunohistochemistry (Paraffin) (IHC (P))

          6 µg/mL
          View 4 publications 4 publications

          Immunohistochemistry (Frozen) (IHC (F))

          -
          View 3 publications 3 publications

          Immunocytochemistry (ICC/IF)

          1 µg/mL
          View 15 publications 15 publications

          Immunoprecipitation (IP)

          -
          View 1 publication 1 publication

          Miscellaneous PubMed (MISC)

          -
          View 3 publications 3 publications
          Product Specifications

          Species Reactivity

          Dog, Human, Mouse, Rat

          Published species

          Chicken, Dog, Human, Mouse, Pig, Rat

          Host/Isotype

          Rabbit / IgG

          Class

          Polyclonal

          Type

          Antibody

          Immunogen

          Synthetic peptide derived from the C-terminal region of the human occludin protein
          View immunogen

          Conjugate

          Unconjugated Unconjugated Unconjugated

          Form

          Liquid

          Concentration

          0.25 mg/mL

          Purification

          Antigen affinity chromatography

          Storage buffer

          PBS, pH 7.4

          Contains

          0.1% sodium azide

          Storage conditions

          -20°C

          Shipping conditions

          Ambient (domestic); Wet ice (international)

          RRID

          AB_2533468

          Product Specific Information

          This antibody is specific for the human occludin protein. On western blots, it identifies the target band at ~65 kDa.

          Reactivity has been confirmed with human Caco-2 and HT29, dog MDCK, mouse TCMK, and rat KNRK cell lysates, and mouse kidney, liver, and rat liver homogenates by western blotting, Caco-2 cells by immunofluorescence, and paraffin-embedded human small intestine tissue by immunohistochemistry.

          For immunofluorescence using Caco-2 cells, fixation with cold ethanol is recommended. For immunohistochemistry in paraffin-embedded tissues, enzyme digestion with pepsin is required prior to staining.

          Target Information

          Occludin is a 65 kDa protein that can exist in a variety of phosphorylated forms, ranging up to approximately 82 kDa. Occludin is thought to be involved in regulating both the localization and the function of occludin. Polyunsaturated fatty acids are known to up-regulate occludin expression, increasing the transendothelial cell resistance and reducing the cellular permeability to large molecules. The level of occludin varies greatly depending on tissue; in brain tissue, occludin is highly and continuously expressed at cell-cell contact sites, whereas non-neural tissues show lower expression and discontinuous distribution. Overall structural features of the occludin protein are highly conserved in all the species examined. Under-expression of tight junction proteins, including occludin, is a key molecular abnormality responsible for the increased permeability of tumor endothelial tight junctions, which contributes to brain tumor edemas.

          For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

          Bioinformatics

          Protein Aliases: Occludin; phosphatase 1, regulatory subunit 115; tight junction protein occludin

          View more View less

          Gene Aliases: AI503564; BLCPMG; Ocl; OCLN; PPP1R115

          View more View less

          UniProt ID: (Dog) Q28269, (Human) Q16625, (Mouse) Q61146, (Rat) Q6P6T5

          View more View less

          Entrez Gene ID: (Dog) 403844, (Human) 100506658, (Mouse) 18260, (Rat) 83497

          View more View less

          Function(s)
          protein binding thiopurine S-methyltransferase activity protein domain specific binding S-adenosylmethionine-dependent methyltransferase activity structural molecule activity
          Process(es)
          bicellular tight junction assembly methylation cell-cell junction organization S-adenosylhomocysteine metabolic process S-adenosylmethionine metabolic process protein complex assembly
          It has to be done as per old AB suggested Products section.
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          If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

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