Bethyl Laboratories

Oct-4 Polyclonal Antibody

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Datasheet
Protocols
Questions & Answers
Datasheet
Protocols
Questions & Answers

Product Details

A304-591A

Applications
Tested Dilution
Publications

Western Blot (WB)

1:1,000-1:5,000
-

Immunoprecipitation (IP)

2-10 µg/mg lysate
-
Product Specifications

Species Reactivity

Mouse

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

Region between residue 1 to 50 of human Octamer-Binding Protein 4.
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Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

1 mg/mL

Purification

Antigen affinity chromatography

Storage buffer

phosphate/tris citrate, pH 7-8

Contains

0.09% sodium azide

Storage conditions

4°C

Shipping conditions

Wet ice

Product Specific Information

The recommended shelf life for this product is 1 year from date of receipt.

Based on 100% sequence identity, this antibody is predicted to react with Human, Bovine, Pig and Monkey

Target Information

POU5F1, also commonly known as Oct-4, is a maternally expressed octamer-binding protein that was the first transcription factor described for the early stages of development. The role of POU5F1 in embryonic development suggested that it might be useful in the creation of stem cells that might be useful in cell replacement therapies in the treatment of several degenerative diseases. Artificial stem cells, termed induced pluripotent stem (iPS) cells, can be created by expressing POU5F1 and the transcription factors Sox2, Klf4 and Lin28 along with c-Myc in mouse fibroblasts. More recently, experiments have demonstrated that iPS cells could be generated using expression plasmids expressing POU5F1, Sox2, KlfF4 and c-Myc, eliminating the need for virus introduction, thereby addressing a safety concern for potential use of iPS cells in regenerative medicine.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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Bioinformatics

Protein Aliases: 3-Oct; 4-Oct; DADB-104B20.2; gp-9; HGNC:9221; MGC22487; NF-A3; Oct-3; Oct-4; Octamer-binding protein 3; Octamer-binding protein 4; Octamer-binding transcription factor 3; OTF-3; OTTHUMP00000221150; OTTHUMP00000221151; POU domain, class 5, transcription factor 1; pou-2; pou2

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Gene Aliases: NF-A3; Oct-3; Oct-3/4; Oct-4; Oct3; Oct3/4; Oct4; Otf-3; Otf-4; Otf3; Otf3-rs7; Otf3g; Otf4; Pou5f1

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UniProt ID: (Mouse) P20263

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Entrez Gene ID: (Mouse) 18999

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Function(s)
RNA polymerase II core promoter proximal region sequence-specific DNA binding RNA polymerase II transcription factor activity, sequence-specific DNA binding transcriptional activator activity, RNA polymerase II core promoter proximal region sequence-specific binding RNA polymerase II transcription coactivator activity DNA binding chromatin binding transcription factor activity, sequence-specific DNA binding transcription corepressor activity protein binding transcription factor binding chromatin DNA binding ubiquitin protein ligase binding miRNA binding sequence-specific DNA binding transcription regulatory region DNA binding poly(A) RNA binding protein heterodimerization activity POU domain binding HMG box domain binding
Process(es)
negative regulation of transcription from RNA polymerase II promoter mesodermal cell fate commitment endodermal cell fate commitment ectodermal cell fate commitment endodermal cell fate specification trophectodermal cell differentiation blastocyst growth BMP signaling pathway involved in heart induction transcription, DNA-templated regulation of transcription, DNA-templated transcription from RNA polymerase II promoter multicellular organism development response to organic substance regulation of gene expression stem cell population maintenance germ-line stem cell population maintenance response to retinoic acid somatic stem cell population maintenance positive regulation of catenin import into nucleus mRNA transcription from RNA polymerase II promoter cell fate commitment negative regulation of cell differentiation negative regulation of transcription, DNA-templated positive regulation of transcription, DNA-templated positive regulation of transcription from RNA polymerase II promoter negative regulation of calcium ion-dependent exocytosis stem cell differentiation positive regulation of protein kinase B signaling negative regulation of protein kinase B signaling positive regulation of SMAD protein import into nucleus cell fate commitment involved in formation of primary germ layer cardiac cell fate determination negative regulation of gene silencing by miRNA regulation of heart induction by regulation of canonical Wnt signaling pathway regulation of methylation-dependent chromatin silencing
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