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Invitrogen
Oligodendrocyte Marker O1 Monoclonal Antibody (O1), eFluor™ 660, eBioscience™
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Product Details
50-6506-82
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Chicken,
Human,
Mouse,
Rat
Published species
Mouse
Host/Isotype
Mouse
/ IgM
Recommended Isotype Control
Class
Monoclonal
Type
Antibody
Clone
O1
Conjugate
Excitation/Emission Max
651/668 nm
View spectra
Form
Liquid
Concentration
0.2 mg/mL
Purification
Affinity chromatography
Storage buffer
PBS, pH 7.2
Contains
0.09% sodium azide
Storage conditions
4°C, store in dark, DO NOT FREEZE!
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
AB_2574269
Product Specific Information
Description: This O1 monoclonal antibody reacts with galactocerebroside and other lipids found on late oligodendrocyte precursors. O1 has been reported to react to all species tested (rat, mouse, human, chicken). Oligodendrocytes function as myelin-producing cells within the central nervous system ensheathing the axons of neurons with myelin in order to facilitate rapid axonal conduction. O1 and O4 monoclonal antibodies serve as stage-specific markers of oligodendrocyte development with O4 marking early oligodendrocyte progenitors and O1-labeling late oligodendrocyte progenitors. O4-positive cells differentiate to become O1-positive, which can in turn become mature oligodendrocytes.
Applications Reported: This O1 antibody has been reported for use in flow cytometric analysis, immunohistochemical staining of frozen tissue sections, and immunocytochemistry.
Applications Tested: This O1 antibody has been tested by immunocytochemistry on fixed, differentiated OLN93 cells and can be used at less than or equal to 20 µg/mL. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.
eFluor® 660 is a replacement for Alexa Fluor® 647. eFluor® 660 emits at 659 nm and is excited with the red laser (633 nm). Please make sure that your instrument is capable of detecting this fluorochome.
Excitation: 633-647 nm; Emission: 668 nm; Laser: Red Laser.
Filtration: 0.2 µm post-manufacturing filtered.
Target Information
Oligodendrocytes function as myelin-producing cells within the central nervous system ensheathing the axons of neurons with myelin in order to facilitate rapid axonal conduction. O1 and O4 monoclonal antibodies serve as stage-specific markers of oligodendrocyte development with O4 marking early oligodendrocyte progenitors and O1-labeling late oligodendrocyte progenitors. O4-positive cells differentiate to become O1-positive, which can in turn become mature oligodendrocytes.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: O1 Marker
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