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          Clicking the images or links will redirect you to a website hosted by BenchSci that provides third-party scientific content. Neither the content nor the BenchSci technology and processes for selection have been evaluated by us; we are providing them as-is and without warranty of any kind, including for use or application of the Thermo Fisher Scientific products presented.

          • Primary Antibodies ›
          • Opsin 4 Antibodies

          Invitrogen

          Opsin 4 Polyclonal Antibody

          Advanced Verification
          25 Published Figures
          33 References
          View all (8) Opsin 4 antibodies

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          Datasheet
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          Datasheet
          Protocols
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          Cite Opsin 4 Polyclonal Antibody

          • Antibody Testing Data (1)
          • Published Figures (25)
          • Advanced Verification (2)
          Opsin 4 Antibody in Immunocytochemistry (ICC/IF)
          Group 53 Created with Sketch.
          Opsin 4 Antibody in Immunocytochemistry (ICC/IF)
          Group 53 Created with Sketch.

          FIGURE: 1 / 28

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          Opsin 4 Antibody (PA1-781) in ICC/IF

          Immunofluorescence staining of melanopsin in rat retinal ganglion cells using Product # PA1-781. {{ $ctrl.currentElement.advancedVerification.fullName }} validation info. View more
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          Opsin 4 Antibody in Immunocytochemistry (ICC/IF)
          Opsin 4 Antibody in Immunocytochemistry (ICC/IF)
          Opsin 4 Antibody in Immunocytochemistry, Immunohistochemistry (ICC/IF, IHC)
          Opsin 4 Antibody in Immunocytochemistry (ICC/IF)
          Opsin 4 Antibody in Immunocytochemistry (ICC/IF)
          Opsin 4 Antibody in Immunocytochemistry (ICC/IF)
          Opsin 4 Antibody in Immunocytochemistry (ICC/IF)
          Opsin 4 Antibody in Immunohistochemistry (IHC)
          Opsin 4 Antibody in Immunohistochemistry (IHC)
          Opsin 4 Antibody in Immunohistochemistry (IHC)
          Opsin 4 Antibody in Immunohistochemistry (IHC)
          Opsin 4 Antibody in Immunohistochemistry (IHC)
          Opsin 4 Antibody in Immunohistochemistry (IHC)
          Opsin 4 Antibody in Immunohistochemistry (IHC)
          Opsin 4 Antibody in Immunohistochemistry (IHC)
          Opsin 4 Antibody in Western Blot (WB)
          Opsin 4 Antibody in Western Blot (WB)
          Opsin 4 Antibody in Western Blot (WB)
          Opsin 4 Antibody in Western Blot (WB)
          Opsin 4 Antibody in Western Blot (WB)
          Opsin 4 Antibody in Western Blot (WB)
          Opsin 4 Antibody in Western Blot (WB)
          Opsin 4 Antibody
          Opsin 4 Antibody

          Product Details

          PA1-781

          Applications
          Tested Dilution
          Publications

          Western Blot (WB)

          0.1-0.2 µg/mL
          View 5 publications 5 publications

          Immunohistochemistry (IHC)

          -
          View 17 publications 17 publications

          Immunohistochemistry (Frozen) (IHC (F))

          1:1,000
          -

          Immunocytochemistry (ICC/IF)

          5 µg/mL
          View 9 publications 9 publications

          Miscellaneous PubMed (MISC)

          -
          View 2 publications 2 publications
          Product Specifications

          Species Reactivity

          Rat

          Published species

          Bat, Human, Mouse, Rat, Rodent, Shrew

          Host/Isotype

          Rabbit / IgG

          Class

          Polyclonal

          Type

          Antibody

          Immunogen

          Synthetic peptide corresponding to residues E(455) Q K S K T P K T K R H L P S L D R R M(474) of rat melanopsin.
          View immunogen

          Conjugate

          Unconjugated Unconjugated Unconjugated

          Form

          Liquid

          Concentration

          1 mg/mL

          Purification

          Antigen affinity chromatography

          Storage buffer

          PBS with 1mg/mL BSA

          Contains

          0.05% sodium azide

          Storage conditions

          -20°C, Avoid Freeze/Thaw Cycles

          Shipping conditions

          Ambient (domestic); Wet ice (international)

          RRID

          AB_2156044

          Product Specific Information

          PA1-781 detects rat melanopsin.

          PA1-781 has been successfully used in Western blot, immunofluorescence, and immunocytochemistry procedures. By Western blot, this antibody detects ~53 and ~85 kDa proteins representing overexpressed unglycosylated and glycosylated melanopsin from HEK 293 cells (does not work well on endogenously expressed protein). By immunofluorescence and immunocytochemistry, PA1-781 detects melanopsin from rat retinal ganglion cells (RGCs).

          The PA1-781 immunogen is a synthetic peptide corresponding to residues E(455) Q K S K T P K T K R H L P S L D R R M(474) of rat melanopsin.

          Target Information

          Melanopsin is an opsin-like protein found in the retinal ganglion cells of mammals. Melanopsin is believed to be part of a secondary optical system that parallels that of the classic rod and cone system. This second system reports directly to the suprachiasmatic nucleus and is responsible for regulation of circadian rhythms. Melanopsin is believed to be the primary photopigment responsible for the regulation of these circadian rhythms, and Melanopsin knockout mice have been generated which demonstrate decreased capacity to entrain to light and dark cycles.

          For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

          Bioinformatics

          Protein Aliases: LOC721663; Melanopsin; MGC142118; OPN 4; opn4 {ECO:0000312|RGD:621701}; Opsin-4

          View more View less

          Gene Aliases: Opn4

          View more View less

          UniProt ID: (Rat) Q8R456

          View more View less

          Entrez Gene ID: (Rat) 192223

          View more View less

          Function(s)
          11-cis retinal binding G-protein coupled photoreceptor activity
          Process(es)
          G-protein coupled receptor signaling pathway visual perception phototransduction detection of visible light rhodopsin mediated signaling pathway protein-chromophore linkage regulation of circadian rhythm rhythmic process
          It has to be done as per old AB suggested Products section.
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          Performance Guarantee

          If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

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