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Invitrogen
Orexin Polyclonal Antibody
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Orexin Antibody (PA5-121242) in WB
Western blot analysis of Orexin in extracts of various cell lines. Samples were incubated with Orexin Polyclonal antibody (Product # PA5-121242) using a dilution of 1:500, followed by HRP Goat Anti-Rabbit IgG (H+L) at a dilution of 1:10,000. Lysates/proteins: 25 µg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit. Exposure time: 180s.
Product Details
PA5-121242
Product Specifications
Species Reactivity
Human,
Mouse,
Rat
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
A synthetic peptide corresponding to a sequence within amino acids 34-66 of human HCRT (NP_0015151)
Conjugate
Form
Liquid
Concentration
1.26 mg/mL
Purification
Affinity Chromatography
Storage buffer
PBS, pH 7.3, with 50% glycerol
Contains
0.02% sodium azide
Storage conditions
-20°C, Avoid Freeze/Thaw Cycles
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
AB_2914814
Product Specific Information
Positive test controls include: U-251MG, Rat thymus. The target is usually found in the following locations: Cell junction, Cytoplasmic vesicle, Rough endoplasmic reticulum, synapse.
Immunogen sequence: QPLPDCCRQK TCSCRLYELL HGAGNHAAGI LTL
Target Information
Neuropeptides that play a significant role in the regulation of food intake and sleep-wakefulness, possibly by coordinating the complex behavioral and physiologic responses of these complementary homeostatic functions. A broader role in the homeostatic regulation of energy metabolism, autonomic function, hormonal balance and the regulation of body fluids, is also suggested. Orexin-A binds to both OX1R and OX2R with a high affinity, whereas orexin-B binds only to OX2R with a similar high affinity.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: Hcrt; Hypocretin; Hypocretin (orexin) neuropeptide; Hypocretin neuropeptide precursor; orexin; orexin A; orexin B; Orexin precursor; Prepro-orexin; Preprohypocretin
Gene Aliases: HCRT; NRCLP1; orexin-A; OX; PPORX; PPOX
UniProt ID: (Human) O43612, (Mouse) O55241, (Rat) O55232
Entrez Gene ID: (Human) 3060, (Mouse) 15171, (Rat) 25723
phospholipase C-activating G-protein coupled receptor signaling pathway
positive regulation of cytosolic calcium ion concentration
protein kinase C-activating G-protein coupled receptor signaling pathway
neuropeptide signaling pathway
chemical synaptic transmission
negative regulation of DNA replication
eating behavior
negative regulation of potassium ion transport
regulation of neurotransmitter secretion
positive regulation of calcium ion transport
negative regulation of transmission of nerve impulse
positive regulation of transmission of nerve impulse
excitatory postsynaptic potential
temperature homeostasis
feeding behavior
behavioral fear response
release of sequestered calcium ion into cytosol
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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